{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:05:41Z","timestamp":1790348741470,"version":"4.1.0"},"reference-count":29,"publisher":"Elsevier BV","issue":"3","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CMP"],"published-print":{"date-parts":[[2021,8,16]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p>The existing parenteral treatment of cervical cancer has high toxicity and\npoor distribution of drugs at the targeted site.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Purpose:<\/jats:title>\n<jats:p>To formulate localized mucoadhesive cisplatin loaded microparticles based formulation\nto treat cervical cancer so that enhanced therapeutics benefits with low toxicity could be achieved.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p> Cisplatin loaded chitosan coated spray-dried microparticles were prepared by ionotropic\ngelation technique and optimized by Central Composite Design. The spray-dried uncoated and chitosan-\ncoated microparticles were characterized for various parameters (Particle size, Morphology,\nDrug entrapment efficiency). In vitro drug release study was carried out in simulated vaginal fluids\nby dialysis membrane method. Ex vivo studies were carried out to evaluate the cytotoxic potential\nof the developed formulation by the MTT assay. A drug permeability study was performed by\nFranz diffusion cell using the vaginal tissue of Swiss Albino Mice.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p>All in vitro characterization parameters were found to be optimum. The in vitro release\nstudies indicated a controlled release following the Higuchi model. The chitosan-coated microparticles\nwere found to be more cytotoxic than uncoated microparticles and plain cisplatin solution. The\nchitosan-coated microparticles were found to be more permeable than uncoated microparticles. Finally,\nin vivo tumor regression and histopathological studies confirmed the significant decrease in\ntumor volume at different time intervals.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>Thus, it can be concluded that mucoadhesive spray-dried microparticles could provide\na favorable approach for localized delivery of the anticancer drug via vaginal route against cervical\ncancer with its enhanced effectiveness.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1874467213666200517120337","type":"journal-article","created":{"date-parts":[[2020,5,17]],"date-time":"2020-05-17T11:17:47Z","timestamp":1589714267000},"page":"381-398","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["DoE Based Optimization and Development of Spray-Dried Chitosan-Coated Alginate Microparticles Loaded with Cisplatin for the Treatment of Cervical Cancer"],"prefix":"10.1016","volume":"14","author":[{"given":"Harpal","family":"Kaur","sequence":"first","affiliation":[{"name":"Department of Pharmaceutics, ISF College of Pharmacy, Moga (Punjab), India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neeraj","family":"Mishra","sequence":"additional","affiliation":[{"name":"Amity Institute of Pharmacy, Amity University of Madhya Pradesh (AUMP), Gwalior (Madhya Pradesh), India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bharat","family":"Khurana","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, ISF College of Pharmacy, Moga (Punjab), India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukhbir","family":"Kaur","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, ISF College of Pharmacy, Moga (Punjab), India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisy","family":"Arora","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, ISF College of Pharmacy, Moga (Punjab), India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"ref=1","first-page":"182","volume":"136","author":"Jacob M.","year":"2012","unstructured":"Jacob M.; Information, education","journal-title":"Indian J Med Res"},{"key":"ref=2","doi-asserted-by":"publisher","first-page":"2893","DOI":"10.1002\/ijc.25516","volume":"127","author":"Ferlay J.","year":"2010","unstructured":"Ferlay J.; Shin H-R.; Bray F.; Forman D.; Mathers C.; Parkin D.M.; Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer 2010,127(12),2893-2917","journal-title":"Int J Cancer"},{"key":"ref=3","year":"2006","unstructured":"Comprehensive Cervical. Cancer Contr World Health Organisation2006","journal-title":"Cancer Contr"},{"key":"ref=4","doi-asserted-by":"publisher","first-page":"2675","DOI":"10.1093\/annonc\/mdr015","volume":"22","author":"Arbyn M.","year":"2011","unstructured":"Arbyn M.; Castellsagu\u00e9 X.; de Sanjos\u00e9 S.; Bruni L.; Saraiya M.; Bray F.; Ferlay J.; Worldwide burden of cervical cancer in 2008. Ann Oncol 2011,22(12),2675-2686","journal-title":"Ann Oncol"},{"key":"ref=5","doi-asserted-by":"publisher","first-page":"1307","DOI":"10.2217\/imt.15.92","volume":"7","author":"Abedin-Do A.","year":"2015","unstructured":"Abedin-Do A.; Taherian-Esfahani Z.; Ghafouri-Fard S.; Ghafouri-Fard S.; Motevaseli E.; Immunomodulatory effects of Lactobacillus strains: emphasis on their effects on cancer cells. Immunotherapy 2015,7(12),1307-1329","journal-title":"Immunotherapy"},{"key":"ref=6","doi-asserted-by":"publisher","first-page":"6645","DOI":"10.1039\/C8DT00838H","volume":"47","author":"Oun R.","year":"2018","unstructured":"Oun R.; Moussa Y.E.; Wheate N.J.; The side effects of platinum-based chemotherapy drugs: a review for chemists. Dalton Trans 2018,47(19),6645-6653","journal-title":"Dalton Trans"},{"key":"ref=7","doi-asserted-by":"publisher","first-page":"57","DOI":"10.3109\/10717544.2012.752419","volume":"20","author":"Khurana B.","year":"2013","unstructured":"Khurana B.; Goyal A.K.; Budhiraja A.; Aora D.; Vyas S.P.; Lipoplexes versus nanoparticles: pDNA\/siRNA delivery. Drug Deliv 2013,20(2),57-64","journal-title":"Drug Deliv"},{"key":"ref=8","doi-asserted-by":"publisher","first-page":"1326","DOI":"10.1023\/A:1011929016601","volume":"15","author":"Illum L.","year":"1998","unstructured":"Illum L.; Chitosan and its use as a pharmaceutical excipient. Pharm Res 1998,15(9),1326-1331","journal-title":"Pharm Res"},{"key":"ref=9","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.msec.2017.02.013","volume":"75","author":"Aggarwal U.","year":"2017","unstructured":"Aggarwal U.; Goyal A.K.; Rath G.; Development and characterization of the cisplatin loaded nanofibers for the treatment of cervical cancer. Mater Sci Eng C 2017,75,125-132","journal-title":"Mater Sci Eng C"},{"key":"ref=10","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1007\/s13346-017-0395-2","volume":"7","author":"Major I.","year":"2017","unstructured":"Major I.; McConville C.; Vaginal drug delivery for the localised treatment of cervical cancer. Drug Deliv Transl Res 2017,7(6),817-828","journal-title":"Drug Deliv Transl Res"},{"key":"ref=11","doi-asserted-by":"publisher","first-page":"879","DOI":"10.3109\/03639040903567109","volume":"36","author":"Onishi H.","year":"2010","unstructured":"Onishi H.; Koyama K.; Sakata O.; Machida Y.; Preparation of chitosan\/alginate\/calcium complex microparticles loaded with lactoferrin and their efficacy on carrageenan-induced edema in rats. Drug Dev Ind Pharm 2010,36(8),879-884","journal-title":"Drug Dev Ind Pharm"},{"key":"ref=12","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/j.ijpharm.2014.06.016","volume":"472","author":"J\u00f8raholmen M.W.","year":"2014","unstructured":"J\u00f8raholmen M.W.; Vani\u0107 Z.; Tho I.; \u0160kalko-Basnet N.; Chitosan-coated liposomes for topical vaginal therapy: Assuring localized drug effect. Int J Pharm 2014,472(1-2),94-101","journal-title":"Int J Pharm"},{"key":"ref=13","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1016\/j.ejpb.2007.04.017","volume":"68","author":"Learoyd T.P.","year":"2008","unstructured":"Learoyd T.P.; Burrows J.L.; French E.; Seville P.C.; Chitosan-based spray-dried respirable powders for sustained delivery of terbutaline sulfate. Eur J Pharm Biopharm 2008,68(2),224-234","journal-title":"Eur J Pharm Biopharm"},{"key":"ref=14","doi-asserted-by":"publisher","first-page":"82","DOI":"10.22159\/ajpcr.2016.v9s2.11814","volume":"9","author":"Shaji J.","year":"2016","unstructured":"Shaji J.; Shaikh M.; Formulation, Optimization, and Characterization of Biocompatible Inhalable D-Cycloserine-Loaded Alginate-Chitosan Nanoparticles for Pulmonary Drug Delivery. Asian J Pharm Clin Res 2016,9,82-95","journal-title":"Asian J Pharm Clin Res"},{"key":"ref=15","doi-asserted-by":"publisher","first-page":"692","DOI":"10.1208\/s12248-016-0007-y","volume":"19","author":"Meng J.","year":"2017","unstructured":"Meng J.; Agrahari V.; Ezoulin M.J.; Purohit S.S.; Zhang T.; Molteni A.; Dim D.; Oyler N.A.; Youan B.C.; Spray-Dried Thiolated Chitosan-Coated Sodium Alginate Multilayer Microparticles for Vaginal HIV Microbicide Delivery. AAPS J 2017,19(3),692-702","journal-title":"AAPS J"},{"key":"ref=16","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1007\/s12602-017-9258-x","volume":"9","author":"Sharma A.","year":"2017","unstructured":"Sharma A.; Arora M.; Goyal A.K.; Rath G.; Spray Dried Formulation of 5-Fluorouracil Embedded with Probiotic Biomass: In Vitro and In Vivo Studies. Probiotics Antimicrob Proteins 2017,9(3),310-322","journal-title":"Probiotics Antimicrob Proteins"},{"key":"ref=17","doi-asserted-by":"publisher","first-page":"S106","DOI":"10.5530\/ijper.50.2.25","volume":"50","author":"Kaushik D.","year":"2016","unstructured":"Kaushik D.; Sharma K.; Sardana S.; Colon Targeting Guar Gum Microspheres of 5-Aminosalicylic Acid: Evaluation of Various Process Variables, Characterization and in-Vitro Drug Release. Indian J Pharm Educ Res 2016,50(2s),S106-S114","journal-title":"Indian J Pharm Educ Res"},{"key":"ref=18","doi-asserted-by":"publisher","first-page":"483","DOI":"10.2147\/DDDT.S99651","volume":"10","author":"Ahmed T.A.","year":"2016","unstructured":"Ahmed T.A.; Aljaeid B.M.; Preparation, characterization, and potential application of chitosan, chitosan derivatives, and chitosan metal nanoparticles in pharmaceutical drug delivery. Drug Des Devel Ther 2016,10,483-507","journal-title":"Drug Des Devel Ther"},{"key":"ref=19","doi-asserted-by":"publisher","first-page":"2403","DOI":"10.2174\/1381612824666180522100251","volume":"24","author":"Arora D.","year":"2018","unstructured":"Arora D.; Khurana B.; Rath G.; Nanda S.; Goyal A.K.; Recent Advances in Nanosuspension Technology for Drug Delivery. Curr Pharm Des 2018,24(21),2403-2415","journal-title":"Curr Pharm Des"},{"key":"ref=20","doi-asserted-by":"publisher","first-page":"6071","DOI":"10.1158\/0008-5472.CAN-09-4442","volume":"70","author":"Huh S.J.","year":"2010","unstructured":"Huh S.J.; Liang S.; Sharma A.; Dong C.; Robertson G.P.; Transiently entrapped circulating tumor cells interact with neutrophils to facilitate lung metastasis development. Cancer Res 2010,70(14),6071-6082","journal-title":"Cancer Res"},{"key":"ref=21","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1023\/A:1020398624602","volume":"19","author":"Quintana A.","year":"2002","unstructured":"Quintana A.; Raczka E.; Piehler L.; Lee I.; Myc A.; Majoros I.; Patri A.K.; Thomas T.; Mule J.; Baker J.R.; Design and Function of a Dendrimer-Based Therapeutic Nanodevice Targeted to Tumor Cells Through the Folate Receptor. Pharm Res 2002,19(9),1310-1316","journal-title":"Pharm Res"},{"key":"ref=22","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1016\/j.ijpharm.2013.06.015","volume":"454","author":"Ravani L.","year":"2013","unstructured":"Ravani L.; Esposito E.; Bories C.; Moal V.L.; Loiseau P.M.; Djabourov M.; Cortesi R.; Bouchemal K.; Clotrimazole-loaded nanostructured lipid carrier hydrogels: thermal analysis and in vitro studies. Int J Pharm 2013,454(2),695-702","journal-title":"Int J Pharm"},{"key":"ref=23","doi-asserted-by":"publisher","first-page":"927","DOI":"10.1002\/app.39247","volume":"130","author":"Dong Z.","year":"2013","unstructured":"Dong Z.; Zheng W.; Xu Z.; Yin Z.; Improved Stability and Tumor Targeting of 5-Fluorouracil by Conjugation with Hyaluronan. J Appl Polym Sci 2013,130(2),927-932","journal-title":"J Appl Polym Sci"},{"key":"ref=24","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.arabjc.2010.12.016","volume":"6","author":"Sunil D.","year":"2013","unstructured":"Sunil D.; Isloor A.M.; Shetty P.; Nayak P.G.; Pai K.S.R.; In Vivo Anticancer and Histopathology Studies of Schiff Bases on Ehrlich Ascitic Carcinoma Cells. 1st Cancer Update. Arab J Chem 2013,6(1),25-33","journal-title":"Arab J Chem"},{"key":"ref=25","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/j.ejpb.2015.03.029","volume":"93","author":"Zong S.","year":"2015","unstructured":"Zong S.; Wang X.; Yang Y.; Wu W.; Li H.; Ma Y.; Lin W.; Sun T.; Huang Y.; Xie Z.; Yue Y.; Liu S.; Jing X.; The use of cisplatin-loaded mucoadhesive nanofibers for local chemotherapy of cervical cancers in mice. Eur J Pharm Biopharm 2015,93,127-135","journal-title":"Eur J Pharm Biopharm"},{"key":"ref=26","first-page":"45","volume":"1","author":"Arora D.","year":"2011","unstructured":"Arora D.; Khurana B.; Kumar M.S.; Vyas S.P.; Oral Immunization against Hepatitis B Virus Using. Int J Recent Adv Pharm Res 2011,1,45-51","journal-title":"Int J Recent Adv Pharm Res"},{"key":"ref=27","first-page":"23","volume":"3","author":"Arora D.","year":"2016","unstructured":"Arora D.; Khurana B.; Narang R.K.; Nanda S.; Quality by Design (QbD) Approach for Optimization and Development of Nano Drug Delivery Systems. Trends Drug Deliv 2016,3(3),23-32","journal-title":"Trends Drug Deliv"},{"key":"ref=28","doi-asserted-by":"publisher","first-page":"359","DOI":"10.2478\/acph-2013-0027","volume":"63","author":"Gupta N.V.","year":"2013","unstructured":"Gupta N.V.; Natasha S.; Getyala A.; Bhat R.S.; Bioadhesive vaginal tablets containing spray dried microspheres loaded with clotrimazole for treatment of vaginal candidiasis. Acta Pharm 2013,63(3),359-372","journal-title":"Acta Pharm"},{"key":"ref=29","first-page":"25","volume":"1","author":"Pahal S.","year":"2011","unstructured":"Pahal S.; Arora D.; Kumar M.S.; Mahadevan N.; Development of Paclitaxel Loaded Immunonanoparticles for HER-2 Positive Breast Cancer Cells. Int J Recent Adv Pharm Res 2011,1,25-39","journal-title":"Int J Recent Adv Pharm Res"}],"container-title":["Current Molecular Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/eurekaselect.com\/article\/download\/182039","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/182039\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eurekaselect.com\/article\/download\/182039","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T22:56:59Z","timestamp":1746572219000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/182039\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,16]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,8,16]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1874467213666200517120337","relation":{},"ISSN":["1874-4672"],"issn-type":[{"value":"1874-4672","type":"print"}],"subject":[],"published":{"date-parts":[[2021,8,16]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2019-12-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-04","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}