{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T19:38:03Z","timestamp":1772998683999,"version":"3.50.1"},"reference-count":67,"publisher":"Elsevier BV","issue":"2","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CP"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:sec>\n<jats:title>Abstract:<\/jats:title>\n<jats:p>Dry Blood Spots (DBS) have been used in combination with liquid chromatography-mass\nspectrometry for targeted proteomics to identify sensitive and specific novel biomarkers. DBS presents\nseveral advantages over other traditional blood sampling methods. This review discusses the\npast, present and future of the technology, focusing on studies with clinical and population relevance.\nArguments for and against DBS are presented by discussing technological advances, particularly those\nrelated to Mass Spectrometry (MS) and Multiple Reaction Monitoring (MRM), sample preparation issues,\ndisease biomarkers, pharmacokinetics, and pharmacodynamics. There will be a focus on proteomic\nstudies that rely on DBS as a sampling method. In this context, numerous studies on the diagnosis\nand treatment of several diseases. To date, proteomic reports of studies using DBS have shown that\nDBS can facilitate diagnosis and prognosis. DBS offers several advantages that make it a viable option\nfor many fields. Moreover, some of its disadvantages can be easily overcome through automation to\nincrease reproducibility and reduce protocol variability and standardization of parameters such as the\nvolume of sample used. Within this context, here we propose to review the advantages and disadvantages\nof using DBS for blood proteomics and provide an understanding of how current DBS-based\nprotocols are being conducted for future standardization and protocol optimization.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1570164620666230727104921","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T05:18:00Z","timestamp":1690780680000},"page":"81-90","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Current Understanding of Dried Spots Platform for Blood Proteomics"],"prefix":"10.1016","volume":"20","author":[{"given":"Luis","family":"Perp\u00e9tuo","sequence":"first","affiliation":[{"name":"iBiMED, Department of Medical Sciences, University of Aveiro, Aveiro, Portugal"},{"name":"Unidade de Investiga\u00e7\u00e3o Cardiovascular, Departamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto, Porto, Portugal"}]},{"given":"Hugo","family":"Rocha","sequence":"additional","affiliation":[{"name":"Newborn Screening, Metabolism and Genetics Unit, Human Genetics Department, National Institute of Health Doutor Ricardo Jorge, 4000-053, Porto, Portugal."}]},{"given":"Rita","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Aveiro, Aveiro, Portugal"}]},{"given":"Bruno","family":"Manadas","sequence":"additional","affiliation":[{"name":"CNC -\nCenter for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal"}]},{"given":"Francisco","family":"Amado","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Aveiro, Aveiro, Portugal"}]},{"given":"Sofia","family":"Guedes","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Aveiro, Aveiro, Portugal"}]},{"given":"Atef","family":"Mahmoud Mannaa","sequence":"additional","affiliation":[{"name":"INSERM U1192, (Dept.) Borg\nAlArab Higher Institute of Engineering and Technology, University of Lille, Lille F-59000, France"},{"name":"Higher Institute\nof Engineering and Technology, New Borg AlArab City, 21934, Alexandria, Egypt"},{"name":"INSERM U1192, Laboratoire Prot\u00e9omique,\nR\u00e9ponse Inflammatoire &amp; Spectrom\u00e9trie de Masse (PRISM), Universit\u00e9 de Lille, Lille F-59000, France"}]},{"given":"Jerome","family":"Vialaret","sequence":"additional","affiliation":[{"name":"IRMB-PPC, INM, University\nof Montpellier, CHU Montpellier, INSERM, CNRS, Montpellier, France"}]},{"given":"Christophe","family":"Hirtz","sequence":"additional","affiliation":[{"name":"IRMB-PPC, INM, University\nof Montpellier, CHU Montpellier, INSERM, CNRS, Montpellier, France"}]},{"given":"Rui","family":"Vitorino","sequence":"additional","affiliation":[{"name":"iBiMED, Department of Medical Sciences, University of Aveiro, Aveiro, Portugal"},{"name":"Unidade de Investiga\u00e7\u00e3o Cardiovascular,\nDepartamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto, Porto, Portugal"}]}],"member":"78","reference":[{"key":"ref=1"},{"key":"ref=1a","doi-asserted-by":"publisher","first-page":"2485","DOI":"10.1007\/s00216-011-5161-6","volume":"402","author":"D\u00e9glon J.","year":"2012","unstructured":"D\u00e9glon J.; Thomas A.; Mangin P.; Staub C.; Direct analysis of dried blood spots coupled with mass spectrometry: Concepts and biomedical applications. Anal Bioanal Chem 2012,402(8),2485-2498","journal-title":"Anal Bioanal Chem"},{"key":"ref=1b","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1542\/peds.102.S1.236","volume":"102","author":"Scriver C.R.","year":"1998","unstructured":"Scriver C.R.; A simple phenylalanine method for detecting phenylketonuria in large populations of newborn infants, by Robert Guthrie and Ada Susi, Pediatrics, 1963;32:318-343. Pediatrics 1998,102(1 Pt 2)(1),236-237","journal-title":"Pediatrics"},{"key":"ref=2a","first-page":"827","volume":"105","author":"Sander J.","year":"1980","unstructured":"Sander J.; Niehaus C.; Deutsche medizinische Wochenschrift (1946). 1980,105(23),827-829"},{"key":"ref=2b","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1016\/S0140-6736(78)91085-1","volume":"311","author":"Farzadegan H.","year":"1978","unstructured":"Farzadegan H.; Noori K.H.; Ala F.; Detection of hepatitis-B surface antigen in blood and blood products dried on filter paper. Lancet 1978,311(8060),362-363","journal-title":"Lancet"},{"key":"ref=3","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1373\/clinchem.2015.247668","volume":"62","author":"George R.S.","year":"2016","unstructured":"George R.S.; Moat S.J.; Effect of dried blood spot quality on newborn screening analyte concentrations and recommendations for minimum acceptance criteria for sample analysis. Clin Chem 2016,62(3),466-475","journal-title":"Clin Chem"},{"key":"ref=4","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1016\/j.phrp.2014.09.005","volume":"5","author":"Choi E.H.","year":"2014","unstructured":"Choi E.H.; Lee S.K.; Ihm C.; Sohn Y.H.; Rapid DNA extraction from dried blood spots on filter paper: Potential applications in biobanking. Osong Public Health Res Perspect 2014,5(6),351-357","journal-title":"Osong Public Health Res Perspect"},{"key":"ref=5","doi-asserted-by":"publisher","first-page":"256","DOI":"10.4269\/ajtmh.17-0889","volume":"99","author":"Lim M.D.","year":"2018","unstructured":"Lim M.D.; Dried blood spots for global health diagnostics and surveillance: Opportunities and challenges. Am J Trop Med Hyg 2018,99(2),256-265","journal-title":"Am J Trop Med Hyg"},{"key":"ref=6","doi-asserted-by":"crossref","first-page":"e23390","DOI":"10.1002\/ajhb.23390","volume":"35","author":"Crimmins E.M.","year":"2020","unstructured":"Crimmins E.M.; Zhang Y.S.; Kim J.K.; Dried blood spots: Effects of less than optimal collection, shipping time, heat, and humidity. Am J Hum Biol 2020,35(5),e23390","journal-title":"Am J Hum Biol"},{"key":"ref=7","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1586\/14789450.2014.965158","volume":"11","author":"Martin N.J.","year":"2014","unstructured":"Martin N.J.; Cooper H.J.; Challenges and opportunities in mass spectrometric analysis of proteins from dried blood spots. Expert Rev Proteomics 2014,11(6),685-695","journal-title":"Expert Rev Proteomics"},{"key":"ref=8a","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1586\/14789450.2014.904204","volume":"11","author":"Nageswara Rao R.","year":"2014","unstructured":"Nageswara Rao R.; Emerging liquid chromatography\u2013mass spectrometry technologies improving dried blood spot analysis. Expert Rev Proteomics 2014,11(4),425-430","journal-title":"Expert Rev Proteomics"},{"key":"ref=8b","doi-asserted-by":"publisher","first-page":"892","DOI":"10.1039\/C5AN02302E","volume":"141","author":"Gaissmaier T.","year":"2016","unstructured":"Gaissmaier T.; Siebenhaar M.; Todorova V.; H\u00fcllen V.; Hopf C.; Therapeutic drug monitoring in dried blood spots using liquid microjunction surface sampling and high resolution mass spectrometry. Analyst 2016,141(3),892-901","journal-title":"Analyst"},{"key":"ref=9a","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1016\/j.jpba.2017.10.007","volume":"148","author":"Xie F.","year":"2018","unstructured":"Xie F.; De Thaye E.; Vermeulen A.; Van Bocxlaer J.; Colin P.; A dried blood spot assay for paclitaxel and its metabolites. J Pharm Biomed Anal 2018,148,307-315","journal-title":"J Pharm Biomed Anal"},{"key":"ref=9b","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1093\/jalm\/jfaa237","volume":"6","author":"Zhao J.","year":"2021","unstructured":"Zhao J.; Sharat C.; Mehta P.A.; Mizuno K.; Vinks A.A.; Setchell K.D.R.; Paperspray ionization mass spectrometry as a tool for predicting real-time optimized dosing of the chemotherapeutic drug melphalan. J Appl Lab Med 2021,6(3),625-636","journal-title":"J Appl Lab Med"},{"key":"ref=10a","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/j.ijantimicag.2018.04.020","volume":"52","author":"Martial L.C.","year":"2018","unstructured":"Martial L.C.; Kerkhoff J.; Martinez N.; Rodr\u00edguez M.; Coronel R.; Molinas G.; Roman M.; Gomez R.; Aguirre S.; Jongedijk E.; Huisman J.; Touw D.J.; P\u00e9rez D.; Chaparro G.; Gonzalez F.; Aarnoutse R.E.; Alffenaar J.W.; Magis-Escurra C.; Evaluation of dried blood spot sampling for pharmacokinetic research and therapeutic drug monitoring of anti-tuberculosis drugs in children. Int J Antimicrob Agents 2018,52(1),109-113","journal-title":"Int J Antimicrob Agents"},{"key":"ref=10b","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1055\/a-1524-0913","volume":"71","author":"Dodin Y.I.","year":"2021","unstructured":"Dodin Y.I.; Suyagh M.F.; Saleh M.I.; Nuseir Z.T.; Aburuz S.M.; Al-Qudah A.A.; Masri A.T.; Younes A.M.; Al-Ghazawi M.A.; Population pharmacokinetics modeling of lamotrigine in jordanian epileptic patients using dried blood spot sampling. Drug Res 2021,71(8),429-437","journal-title":"Drug Res"},{"key":"ref=11","doi-asserted-by":"publisher","first-page":"3765","DOI":"10.1007\/s00216-020-02634-4","volume":"412","author":"Lange T.","year":"2020","unstructured":"Lange T.; Thomas A.; Walpurgis K.; Thevis M.; Fully automated dried blood spot sample preparation enables the detection of lower molecular mass peptide and non-peptide doping agents by means of LC-HRMS. Anal Bioanal Chem 2020,412(15),3765-3777","journal-title":"Anal Bioanal Chem"},{"key":"ref=12","doi-asserted-by":"publisher","first-page":"1961","DOI":"10.1373\/clinchem.2008.111989","volume":"54","author":"deWilde A.","year":"2008","unstructured":"deWilde A.; Sadilkova K.; Sadilek M.; Vasta V.; Hahn S.H.; Tryptic peptide analysis of ceruloplasmin in dried blood spots using liquid chromatography-tandem mass spectrometry: Application to newborn screening. Clin Chem 2008,54(12),1961-1968","journal-title":"Clin Chem"},{"key":"ref=13","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1074\/mcp.TIR119.001820","volume":"19","author":"Eshghi A.","year":"2020","unstructured":"Eshghi A.; Pistawka A.J.; Liu J.; Chen M.; Sinclair N.J.T.; Hardie D.B.; Elliott M.; Chen L.; Newman R.; Mohammed Y.; Borchers C.H.; Concentration determination of >200 proteins in dried blood spots for biomarker discovery and validation. Mol Cell Proteomics 2020,19(3),540-553","journal-title":"Mol Cell Proteomics"},{"key":"ref=14","doi-asserted-by":"publisher","first-page":"1700362","DOI":"10.1002\/ejlt.201700362","volume":"120","author":"Hewawasam E.","year":"2018","unstructured":"Hewawasam E.; Liu G.; Jeffery D.W.; Gibson R.A.; Muhlhausler B.S.; Estimation of the volume of blood in a small disc punched from a dried blood spot card. Eur J Lipid Sci Technol 2018,120(3),1700362","journal-title":"Eur J Lipid Sci Technol"},{"key":"ref=15a","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/j.clinbiochem.2022.03.007","volume":"104","author":"Francke M.I.","year":"2022","unstructured":"Francke M.I.; van Domburg B.; van de Velde D.; Hesselink D.A.; de Winter B.C.M.; The use of freeze-dried blood samples affects the results of a dried blood spot analysis. Clin Biochem 2022,104,70-73","journal-title":"Clin Biochem"},{"key":"ref=15b","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1089\/bio.2020.0131","volume":"19","author":"Carpentieri D.","year":"2021","unstructured":"Carpentieri D.; Colvard A.; Petersen J.; Marsh W.; David-Dirgo V.; Huentelman M.; Pirrotte P.; Sivakumaran T.A.; Mind the quality gap when banking on dry blood spots. Biopreserv Biobank 2021,19(2),136-142","journal-title":"Biopreserv Biobank"},{"key":"ref=15c","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1002\/dta.1582","volume":"6","author":"Rinc\u00f3n J.P.","year":"2014","unstructured":"Rinc\u00f3n J.P.; Meesters R.J.W.; Evaluation of peripheral blood microsampling techniques in combination with liquid chromatography-high resolution mass spectrometry for the determination of drug pharmacokinetics in clinical studies. Drug Test Anal 2014,6(6),568-577","journal-title":"Drug Test Anal"},{"key":"ref=16","first-page":"1207","volume":"412","author":"Elbin C. S.","year":"2011","unstructured":"Elbin C. S.; Olivova P.; Marashio C. A.; Cooper S. K.; Cullen E.; Keutzer J. M.; Zhang X. K.; Clinica chimica acta. int j cli chem 2011,412(13-14),1207-1212","journal-title":"int j cli chem"},{"key":"ref=17","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1016\/j.scitotenv.2014.06.142","volume":"494-495","author":"Batterman S.","year":"2014","unstructured":"Batterman S.; Chernyak S.; Performance and storage integrity of dried blood spots for PCB, BFR and pesticide measurements. Sci Total Environ 2014,494-495,252-260","journal-title":"Sci Total Environ"},{"key":"ref=18a","first-page":"e9121","volume":"35","author":"Okai C. A.","year":"2021","unstructured":"Okai C. A.; W\u00f6lter M.; Russ M.; Koy C.; Petre B. A.; Rath W.; Pecks U.; Glocker M. O.;  2021,35(14),e9121"},{"key":"ref=18b","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1080\/19485565.2017.1283582","volume":"63","author":"Eick G.N.","year":"2017","unstructured":"Eick G.N.; Kowal P.; Barrett T.; Thiele E.A.; Snodgrass J.J.; Enzyme-linked immunoassay-based quantitative measurement of apolipoprotein B (ApoB) in dried blood spots, a biomarker of cardiovascular disease risk. Biodemogr Soc Biol 2017,63(2),116-130","journal-title":"Biodemogr Soc Biol"},{"key":"ref=19","doi-asserted-by":"publisher","first-page":"1060","DOI":"10.1373\/clinchem.2005.049270","volume":"51","author":"Freer D.E.","year":"2005","unstructured":"Freer D.E.; Observations on heat\/humidity denaturation of enzymes in filter-paper blood spots from newborns. Clin Chem 2005,51(6),1060-1062","journal-title":"Clin Chem"},{"key":"ref=20","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1093\/jac\/dkp150","volume":"64","author":"Garc\u00eda-Lerma J.G.","year":"2009","unstructured":"Garc\u00eda-Lerma J.G.; McNulty A.; Jennings C.; Huang D.; Heneine W.; Bremer J.W.; Rapid decline in the efficiency of HIV drug resistance genotyping from dried blood spots (DBS) and dried plasma spots (DPS) stored at 37 C and high humidity. J Antimicrob Chemother 2009,64(1),33-36","journal-title":"J Antimicrob Chemother"},{"key":"ref=21","doi-asserted-by":"publisher","first-page":"1034","DOI":"10.1373\/clinchem.2008.111641","volume":"55","author":"Buxton O.M.","year":"2009","unstructured":"Buxton O.M.; Malarick K.; Wang W.; Seeman T.; Changes in dried blood spot Hb A1c with varied postcollection conditions. Clin Chem 2009,55(5),1034-1036","journal-title":"Clin Chem"},{"key":"ref=22","doi-asserted-by":"publisher","first-page":"652","DOI":"10.1373\/clinchem.2003.029488","volume":"50","author":"McDade T.W.","year":"2004","unstructured":"McDade T.W.; Burhop J.; Dohnal J.; High-sensitivity enzyme immunoassay for C-reactive protein in dried blood spots. Clin Chem 2004,50(3),652-654","journal-title":"Clin Chem"},{"key":"ref=23","doi-asserted-by":"publisher","first-page":"1286","DOI":"10.1074\/mcp.RA117.000015","volume":"16","author":"Bj\u00f6rkesten J.","year":"2017","unstructured":"Bj\u00f6rkesten J.; Enroth S.; Shen Q.; Wik L.; Hougaard D.M.; Cohen A.S.; S\u00f6rensen L.; Giedraitis V.; Ingelsson M.; Larsson A.; Kamali-Moghaddam M.; Landegren U.; Stability of proteins in dried blood spot biobanks. Mol Cell Proteomics 2017,16(7),1286-1296","journal-title":"Mol Cell Proteomics"},{"key":"ref=24","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.ymgme.2015.06.011","volume":"116","author":"Grauholm J.","year":"2015","unstructured":"Grauholm J.; Khoo S.K.; Nickolov R.Z.; Poulsen J.B.; B\u00e6kvad-Hansen M.; Hansen C.S.; Hougaard D.M.; Hollegaard M.V.; Gene expression profiling of archived dried blood spot samples from the danish neonatal screening biobank. Mol Genet Metab 2015,116(3),119-124","journal-title":"Mol Genet Metab"},{"key":"ref=25","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1016\/j.biopsych.2020.09.005","volume":"89","author":"Gardner R.M.","year":"2021","unstructured":"Gardner R.M.; Lee B.K.; Brynge M.; Sj\u00f6qvist H.; Dalman C.; Karlsson H.; Neonatal levels of acute phase proteins and risk of autism spectrum disorder. Biol Psychiatry 2021,89(5),463-475","journal-title":"Biol Psychiatry"},{"key":"ref=26","doi-asserted-by":"publisher","first-page":"113844","DOI":"10.1016\/j.jpba.2020.113844","volume":"195","author":"Lai Y.","year":"2021","unstructured":"Lai Y.; Zhang G.; Inhaber N.; Bernstein J.A.; Cwik M.; Zhou Z.; Chockalingam P.S.; Wu J.; A robust multiplexed assay to quantify C1-inhibitor, C1q, and C4 proteins for in vitro diagnosis of hereditary angioedema from dried blood spot. J Pharm Biomed Anal 2021,195,113844","journal-title":"J Pharm Biomed Anal"},{"key":"ref=27a","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1002\/prca.201400042","volume":"8","author":"Ignjatovic V.","year":"2014","unstructured":"Ignjatovic V.; Pitt J.; Monagle P.; Craig J.M.; The utility of dried blood spots for proteomic studies: Looking forward to looking back. Proteomics Clin Appl 2014,8(11-12),896-900","journal-title":"Proteomics Clin Appl"},{"key":"ref=27b","doi-asserted-by":"publisher","first-page":"1152","DOI":"10.3389\/fnins.2019.01152","volume":"13","author":"Anderson D.N.","year":"2019","unstructured":"Anderson D.N.; Anderson C.; Lanka N.; Sharma R.; Butson C.R.; Baker B.W.; Dorval A.D.; The \u03bcDBS: Multiresolution, directional deep brain stimulation for improved targeting of small diameter fibers. Front Neurosci 2019,13,1152","journal-title":"Front Neurosci"},{"key":"ref=27c","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.ab.2019.03.006","volume":"575","author":"Hogeling S.M.","year":"2019","unstructured":"Hogeling S.M.; Cox M.T.; Bradshaw R.M.; Smith D.P.; Duckett C.J.; Quantification of proteins in whole blood, plasma and DBS, with element-labelled antibody detection by ICP-MS. Anal Biochem 2019,575,10-16","journal-title":"Anal Biochem"},{"key":"ref=28a","doi-asserted-by":"crossref","first-page":"2821","DOI":"10.1021\/acs.jproteome.0c00271","volume":"19","author":"Nakajima D.","year":"2020","unstructured":"Nakajima D.; Kawashima Y.; Simple and sensitive analysis for dried blood spot proteins by sodium carbonate precipitation for clinical proteomics. J Proteome Res 2020,19(7),2821-2827","journal-title":"J Proteome Res"},{"key":"ref=28b","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1007\/978-1-0716-1936-0_4","volume":"2420","author":"Nakajima D.","year":"2022","unstructured":"Nakajima D.; Ohara O.; Kawashima Y.; Data-independent acquisition mass spectrometry-based deep proteome analysis for hydrophobic proteins from dried blood spots enriched by sodium carbonate precipitation. Methods Mol Biol 2022,2420,39-52","journal-title":"Methods Mol Biol"},{"key":"ref=29","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1021\/acs.jproteome.7b00746","volume":"17","author":"Rosting C.","year":"2018","unstructured":"Rosting C.; Yu J.; Cooper H.J.; High field asymmetric waveform ion mobility. SpectroJ Proteome Res 2018,17(6),1997-2004","journal-title":"SpectroJ Proteome Res"},{"key":"ref=30","doi-asserted-by":"publisher","first-page":"1242","DOI":"10.1007\/s13361-013-0658-1","volume":"24","author":"Martin N.J.","year":"2013","unstructured":"Martin N.J.; Bunch J.; Cooper H.J.; Dried blood spot proteomics: surface extraction of endogenous proteins coupled with automated sample preparation and mass spectrometry analysis. J Am Soc Mass Spectrom 2013,24(8),1242-1249","journal-title":"J Am Soc Mass Spectrom"},{"key":"ref=31","doi-asserted-by":"publisher","first-page":"1921","DOI":"10.1007\/s13361-012-0477-9","volume":"23","author":"Edwards R.L.","year":"2012","unstructured":"Edwards R.L.; Griffiths P.; Bunch J.; Cooper H.J.; Top-down proteomics and direct surface sampling of neonatal dried blood spots: Diagnosis of unknown hemoglobin variants. J Am Soc Mass Spectrom 2012,23(11),1921-1930","journal-title":"J Am Soc Mass Spectrom"},{"key":"ref=32","doi-asserted-by":"publisher","first-page":"1232","DOI":"10.1002\/pmic.201300316","volume":"14","author":"Edwards R.L.","year":"2014","unstructured":"Edwards R.L.; Griffiths P.; Bunch J.; Cooper H.J.; Compound heterozygotes and beta-thalassemia: Top-down mass spectrometry for detection of hemoglobinopathies. Proteomics 2014,14(10),1232-1238","journal-title":"Proteomics"},{"key":"ref=33","doi-asserted-by":"publisher","first-page":"1338","DOI":"10.1007\/s13361-013-0678-x","volume":"24","author":"Chambers A.G.","year":"2013","unstructured":"Chambers A.G.; Percy A.J.; Hardie D.B.; Borchers C.H.; Comparison of proteins in whole blood and dried blood spot samples by LC\/MS\/MS. J Am Soc Mass Spectrom 2013,24(9),1338-1345","journal-title":"J Am Soc Mass Spectrom"},{"key":"ref=34","doi-asserted-by":"publisher","first-page":"3903","DOI":"10.1038\/s41467-020-17643-1","volume":"11","author":"Tiambeng T.N.","year":"2020","unstructured":"Tiambeng T.N.; Roberts D.S.; Brown K.A.; Zhu Y.; Chen B.; Wu Z.; Mitchell S.D.; Guardado-Alvarez T.M.; Jin S.; Ge Y.; Nanoproteomics enables proteoform-resolved analysis of low-abundance proteins in human serum. Nat Commun 2020,11(1),3903","journal-title":"Nat Commun"},{"key":"ref=35","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1038\/nmeth.2369","volume":"10","author":"Smith L.M.","year":"2013","unstructured":"Smith L.M.; Kelleher N.L.; Proteoform: A single term describing protein complexity. Nat Methods 2013,10(3),186-187","journal-title":"Nat Methods"},{"key":"ref=36","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1074\/mcp.M112.022442","volume":"12","author":"Chambers A.G.","year":"2013","unstructured":"Chambers A.G.; Percy A.J.; Yang J.; Camenzind A.G.; Borchers C.H.; Multiplexed quantitation of endogenous proteins in dried blood spots by multiple reaction monitoring-mass spectrometry. Mol Cell Proteomics 2013,12(3),781-791","journal-title":"Mol Cell Proteomics"},{"key":"ref=37","doi-asserted-by":"publisher","first-page":"45178","DOI":"10.1038\/srep45178","volume":"7","author":"Ozcan S.","year":"2017","unstructured":"Ozcan S.; Cooper J.D.; Lago S.G.; Kenny D.; Rustogi N.; Stocki P.; Bahn S.; Towards reproducible MRM based biomarker discovery using dried blood spots. Sci Rep 2017,7(1),45178","journal-title":"Sci Rep"},{"key":"ref=38","doi-asserted-by":"publisher","first-page":"3094","DOI":"10.1074\/mcp.O115.049957","volume":"14","author":"Chambers A.G.","year":"2015","unstructured":"Chambers A.G.; Percy A.J.; Yang J.; Borchers C.H.; Multiple reaction monitoring enables precise quantification of 97 proteins in dried blood spots. Mol Cell Proteomics 2015,14(11),3094-3104","journal-title":"Mol Cell Proteomics"},{"key":"ref=39","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1007\/978-1-62703-405-0_13","volume":"1000","author":"Percy A.J.","year":"2013","unstructured":"Percy A.J.; Chambers A.G.; Parker C.E.; Borchers C.H.; Absolute quantitation of proteins in human blood by multiplexed multiple reaction monitoring mass spectrometry. Methods Mol Biol 2013,1000,167-189","journal-title":"Methods Mol Biol"},{"key":"ref=40a","doi-asserted-by":"publisher","first-page":"465","DOI":"10.4103\/ijp.IJP_578_16","volume":"49","author":"Jain R.","year":"2017","unstructured":"Jain R.; Quraishi R.; Ambekar A.; Verma A.; Gupta P.; Dried urine spots for detection of benzodiazepines. Indian J Pharmacol 2017,49(6),465-469","journal-title":"Indian J Pharmacol"},{"key":"ref=40b","doi-asserted-by":"publisher","first-page":"5334","DOI":"10.3390\/molecules26175334","volume":"26","author":"Moretti M.","year":"2021","unstructured":"Moretti M.; Freni F.; Carelli C.; Previder\u00e9 C.; Grignani P.; Vignali C.; Cobo-Golpe M.; Morini L.; Analysis of cannabinoids and metabolites in dried urine spots (DUS). Molecules 2021,26(17),5334","journal-title":"Molecules"},{"key":"ref=41","doi-asserted-by":"publisher","first-page":"R80","DOI":"10.1186\/gb-2006-7-9-r80","volume":"7","author":"Adachi J.","year":"2006","unstructured":"Adachi J.; Kumar C.; Zhang Y.; Olsen J.V.; Mann M.; The human urinary proteome contains more than 1500 proteins, including a large proportion of membrane proteins. Genome Biol 2006,7(9),R80","journal-title":"Genome Biol"},{"key":"ref=42","author":"Haider M.Z.","year":"2022","unstructured":"Haider M.Z.; Aslam A.; Proteinuria. StatPearls, StatPearls Publishing Copyright \u00a9 2022 2022","journal-title":"StatPearls, StatPearls Publishing Copyright \u00a9 2022"},{"key":"ref=43a","doi-asserted-by":"publisher","first-page":"2803","DOI":"10.4155\/bio.14.197","volume":"6","author":"Carre\u00f1o Balc\u00e1zar J.S.","year":"2014","unstructured":"Carre\u00f1o Balc\u00e1zar J.S.; Meesters R.J.W.; Bioanalytical comparison between dried urine spots and liquid urine bioassays used for the quantitative analysis of urinary creatinine concentrations. Bioanalysis 2014,6(21),2803-2814","journal-title":"Bioanalysis"},{"key":"ref=43b","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1080\/14789450.2018.1444483","volume":"15","author":"Preece R.L.","year":"2018","unstructured":"Preece R.L.; Han S.Y.S.; Bahn S.; Proteomic approaches to identify blood-based biomarkers for depression and bipolar disorders. Expert Rev Proteomics 2018,15(4),325-340","journal-title":"Expert Rev Proteomics"},{"key":"ref=44a","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1016\/j.jprot.2016.12.008","volume":"154","author":"Yu C.","year":"2017","unstructured":"Yu C.; Huang S.; Wang M.; Zhang J.; Liu H.; Yuan Z.; Wang X.; He X.; Wang J.; Zou L.; A novel tandem mass spectrometry method for first-line screening of mainly beta-thalassemia from dried blood spots. J Proteomics 2017,154,78-84","journal-title":"J Proteomics"},{"key":"ref=44b","doi-asserted-by":"publisher","first-page":"1290","DOI":"10.1038\/s41398-017-0027-0","volume":"7","author":"Cooper J.D.","year":"2017","unstructured":"Cooper J.D.; Ozcan S.; Gardner R.M.; Rustogi N.; Wicks S.; van Rees G.F.; Leweke F.M.; Dalman C.; Karlsson H.; Bahn S.; Schizophrenia-risk and urban birth are associated with proteomic changes in neonatal dried blood spots. Transl Psychiatry 2017,7(12),1290","journal-title":"Transl Psychiatry"},{"key":"ref=44c","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1002\/rcm.6823","volume":"28","author":"Han M.","year":"2014","unstructured":"Han M.; Jun S.H.; Song S.H.; Park H.D.; Park K.U.; Song J.; Ultra-performance liquid chromatography\/tandem mass spectrometry for determination of sulfatides in dried blood spots from patients with metachromatic leukodystrophy. Rapid Commun Mass Spectrom 2014,28(6),587-594","journal-title":"Rapid Commun Mass Spectrom"},{"key":"ref=44d","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1007\/s00216-010-3952-9","volume":"398","author":"Meesters R.J.W.","year":"2010","unstructured":"Meesters R.J.W.; van Kampen J.J.A.; Reedijk M.L.; Scheuer R.D.; Dekker L.J.M.; Burger D.M.; Hartwig N.G.; Osterhaus A.D.M.E.; Luider T.M.; Gruters R.A.; Ultrafast and high-throughput mass spectrometric assay for therapeutic drug monitoring of antiretroviral drugs in pediatric HIV-1 infection applying dried blood spots. Anal Bioanal Chem 2010,398(1),319-328","journal-title":"Anal Bioanal Chem"},{"key":"ref=44e","doi-asserted-by":"publisher","first-page":"343","DOI":"10.3390\/ijms17030343","volume":"17","author":"Krone C.","year":"2016","unstructured":"Krone C.; Oja A.; van de Groep K.; Sanders E.; Bogaert D.; Trzci\u0144ski K.; Dried saliva spots: A robust method for detecting streptococcus pneumoniae carriage by PCR. Int J Mol Sci 2016,17(3),343","journal-title":"Int J Mol Sci"},{"key":"ref=44f","doi-asserted-by":"publisher","first-page":"211","DOI":"10.1016\/j.jpba.2013.02.017","volume":"78-79","author":"Rao R.N.","year":"2013","unstructured":"Rao R.N.; Prasad K.G.; Naidu C.G.; Saida S.; Agwane S.B.; Development of a validated LC-MS\/MS method for determination of doxofylline on rat dried blood spots and urine: Application to pharmacokinetics. J Pharm Biomed Anal 2013,78-79,211-216","journal-title":"J Pharm Biomed Anal"},{"key":"ref=45","doi-asserted-by":"publisher","first-page":"656","DOI":"10.1373\/clinchem.2017.275966","volume":"64","author":"Freeman J.D.","year":"2018","unstructured":"Freeman J.D.; Rosman L.M.; Ratcliff J.D.; Strickland P.T.; Graham D.R.; Silbergeld E.K.; State of the science in dried blood spots. Clin Chem 2018,64(4),656-679","journal-title":"Clin Chem"},{"key":"ref=46","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.clinbiochem.2020.02.007","volume":"82","author":"Luginb\u00fchl M.","year":"2020","unstructured":"Luginb\u00fchl M.; Gaugler S.; The application of fully automated dried blood spot analysis for liquid chromatography-tandem mass spectrometry using the CAMAG DBS-MS 500 autosampler. Clin Biochem 2020,82,33-39","journal-title":"Clin Biochem"},{"key":"ref=47","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1080\/10408363.2017.1297358","volume":"54","author":"Lehmann S.","year":"2017","unstructured":"Lehmann S.; Picas A.; Tiers L.; Vialaret J.; Hirtz C.; Clinical perspectives of dried blood spot protein quantification using mass spectrometry methods. Crit Rev Clin Lab Sci 2017,54(3),173-184","journal-title":"Crit Rev Clin Lab Sci"},{"key":"ref=48","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1002\/mas.21441","volume":"35","author":"Wagner M.","year":"2016","unstructured":"Wagner M.; Tonoli D.; Varesio E.; Hopfgartner G.; The use of mass spectrometry to analyze dried blood spots. Mass Spectrom Rev 2016,35(3),361-438","journal-title":"Mass Spectrom Rev"}],"container-title":["Current Proteomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.eurekaselect.com\/article\/download?doi=10.2174\/1570164620666230727104921","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/219202\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/article\/download?doi=10.2174\/1570164620666230727104921","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T17:21:59Z","timestamp":1743182519000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/219202\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4]]},"references-count":67,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,4]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1570164620666230727104921","relation":{},"ISSN":["1570-1646"],"issn-type":[{"value":"1570-1646","type":"print"}],"subject":[],"published":{"date-parts":[[2023,4]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2023-03-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-30","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}