{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T04:15:13Z","timestamp":1763957713348,"version":"3.45.0"},"reference-count":46,"publisher":"Bentham Science Publishers Ltd.","issue":"6","funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/ BIM-MED\/3363\/2014","PD\/BD\/136919\/2018"],"award-info":[{"award-number":["PTDC\/ BIM-MED\/3363\/2014","PD\/BD\/136919\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"iNOVA4Health","award":["UID\/Multi\/04462\/2013"],"award-info":[{"award-number":["UID\/Multi\/04462\/2013"]}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CSCR"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Dand5 encodes a protein that acts as an antagonist to Nodal\/TGF-\u03b2 and Wnt pathways. A mouse knockout (KO) model has shown that this molecule is associated with left-right asymmetry and cardiac development, with its depletion causing heterotaxia and cardiac hyperplasia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>This study aimed to investigate the molecular mechanisms affected by the depletion of Dand5.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>DAND5-KO and wild-type embryoid bodies (EBs) were used to assess genetic expression with RNA sequencing. To complement the expression results that pointed towards differences in epithelial to mesenchymal transition (EMT), we evaluated migration and cell attachment. Lastly, in vivo valve development was investigated, as it was an established model of EMT.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>DAND5-KO EBs progress faster through differentiation. The differences in expression will lead to differences in the expression of genes involved with Notch and Wnt signalling pathways, as well as changes in the expression of genes encoding membrane proteins. Such changes were accompanied by lower migratory rates in DAND5-KO EBs, as well as higher concentrations of focal adhesions. Within valve development, Dand5 is expressed in the myocardium underlying future valve sites, and its depletion compromises correct valve structure.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>The DAND5 range of action goes beyond early development. Its absence leads to significantly different expression patterns in vitro and defects in EMT and migration. These results have an in vivo translation in mouse heart valve development. Knowledge regarding the influence of DAND5 in EMT and cell transformation allows further understanding of its role in development, or even in some disease contexts, such as congenital heart defects.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2174\/1574888x18666230516154113","type":"journal-article","created":{"date-parts":[[2023,5,17]],"date-time":"2023-05-17T01:20:27Z","timestamp":1684286427000},"page":"673-684","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Depletion of\n                    <i>DAND5<\/i>\n                    Hinders EMT in Mouse Embryonic Stem Cell Differentiation"],"prefix":"10.2174","volume":"20","author":[{"given":"Joao","family":"von Gilsa Lopes","sequence":"first","affiliation":[{"name":"Stem Cells and Development Laboratory, iNOVA4Health, NOVA Medical School Faculdade de Ci\u00eancias M\u00e9dicas,NMS\/FCM, Universidade Nova de Lisboa, Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9790-8335","authenticated-orcid":true,"given":"Jose Manuel","family":"Inacio","sequence":"additional","affiliation":[{"name":"Stem Cells and Development Laboratory, iNOVA4Health, NOVA Medical School Faculdade de Ci\u00eancias M\u00e9dicas,NMS\/FCM, Universidade Nova de Lisboa, Lisboa, Portugal"}]},{"given":"Sara","family":"Marques","sequence":"additional","affiliation":[{"name":"Stem Cells and Development Laboratory, iNOVA4Health, NOVA Medical School Faculdade de Ci\u00eancias M\u00e9dicas,NMS\/FCM, Universidade Nova de Lisboa, Lisboa, Portugal"}]},{"given":"Sabrina Brito","family":"Anez","sequence":"additional","affiliation":[{"name":"Stem Cells and Development Laboratory, iNOVA4Health, NOVA Medical School Faculdade de Ci\u00eancias M\u00e9dicas,NMS\/FCM, Universidade Nova de Lisboa, Lisboa, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7384-0949","authenticated-orcid":true,"given":"Jose Antonio","family":"Belo","sequence":"additional","affiliation":[{"name":"Stem Cells and Development Laboratory, iNOVA4Health, NOVA Medical School Faculdade de Ci\u00eancias M\u00e9dicas,NM"},{"name":"FCM, Universidade Nova de Lisboa, Lisboa, Portugal"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1002\/dvdy.23711","volume":"241","author":"Williams M.","year":"2012","unstructured":"Williams M.; Burdsal C.; Periasamy A.; Lewandoski M.; Sutherland A.; Mouse primitive streak forms in situ by initiation of epithelial to mesenchymal transition without migration of a cell population. 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