{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T19:00:36Z","timestamp":1774292436187,"version":"3.50.1"},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"4","content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["ACAMC"],"published-print":{"date-parts":[[2018,7,17]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p> An ideal strategy for cancer treatment is the specific induction of tumor cell death, sparing\nnormal cells. Marine sponges are rich biological reservoirs of biomolecules, especially lectins, which have attracted\nconsiderable attention due to potential biological effect on human cells. Lectins are proteins that bind specific\ncarbohydrate signatures and some gained further interest for their capacity to bind tumor associated carbohydrates\nantigens and induce tumor cell apoptosis.\n<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Objective:<\/jats:title>\n<jats:p> This study aimed to evaluate the antitumor potential of H3, a lectin, recently reported from marine sponge\nHaliclona caerulea on the human breast cancer cell line MCF7.\n<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Results:<\/jats:title>\n<jats:p> H3 reduced MCF7 cell viability with an IC50 of 100 \u00b5g\/ml, without a significant effect on normal cells. At\n24 h, H3 induced a significant arrest in the G1 cell cycle phase. Consistently, almost 50% of the cells were in early\napoptosis and showed remarkable increased expression of caspase-9 (CASP 9). H3 impaired dramatically the\nadhesiveness of MCF7 cells in culture. Assays conducted with Lysotracker Red probe showed increased organelle\nacidity, suggesting autophagic cell death, which was further supported by increased expression of microtubuleassociated\nprotein light chain 3 (LC3) and observable conversion of LC3-I in LC3-II by western blot.\n<\/jats:p><\/jats:sec>\n<jats:sec>\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>  The apoptotic effect of H3 may be related to a balance between apoptotic and autophagic cell death,\nmediated by increased expression of CASP 9 and LC3-II. To the best of our knowledge this is the first report about a\nsponge lectin triggering both apoptosis and autophagy in MCF7 cell.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/1871520617666171114094847","type":"journal-article","created":{"date-parts":[[2018,6,5]],"date-time":"2018-06-05T04:48:27Z","timestamp":1528174107000},"page":"521-528","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Halilectin-3, a Lectin from the Marine Sponge Haliclona caerulea, Induces Apoptosis and Autophagy in Human Breast Cancer MCF7 Cells Through Caspase-9 Pathway and LC3-II Protein Expression"],"prefix":"10.2174","volume":"18","author":[{"given":"Luiz G.","family":"do Nascimento-Neto","sequence":"first","affiliation":[{"name":"CEDOC, NOVA Medical School, Universidade NOVA de Lisboa, 1150-082, Lisbon, Portugal"}]},{"given":"Maria G.","family":"Cabral","sequence":"additional","affiliation":[{"name":"CEDOC, NOVA Medical School, Universidade NOVA de Lisboa, 1150-082, Lisbon, Portugal"}]},{"given":"Romulo F.","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Laboratorio de Biotecnologia Marinha (BioMar-Lab), Departamento de Engenharia de Pesca, Universidade Federal do Ceara, Campus do Pici S\/N, Bloco 871, 60.440-970, Fortaleza, Ceara, Brazil"}]},{"given":"Zelia","family":"Silva","sequence":"additional","affiliation":[{"name":"UCIBIO, Departamento de Ciencias da Vida, Faculdade de Ciencias e Tecnologia, Universidade NOVA de Lisboa, 2829-516, Caparica, Portugal"}]},{"given":"Francisco V.S.","family":"Arruda","sequence":"additional","affiliation":[{"name":"CEDOC, NOVA Medical School, Universidade NOVA de Lisboa, 1150-082, Lisbon, Portugal"}]},{"given":"Celso S.","family":"Nagano","sequence":"additional","affiliation":[{"name":"Laboratorio de Biotecnologia Marinha (BioMar-Lab), Departamento de Engenharia de Pesca, Universidade Federal do Ceara, Campus do Pici S\/N, Bloco 871, 60.440-970, Fortaleza, Ceara, Brazil"}]},{"given":"Alexandra R.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"UCIBIO, Departamento de Ciencias da Vida, Faculdade de Ciencias e Tecnologia, Universidade NOVA de Lisboa, 2829-516, Caparica, Portugal"}]},{"given":"Alexandre H.","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Laboratorio de Biotecnologia Marinha (BioMar-Lab), Departamento de Engenharia de Pesca, Universidade Federal do Ceara, Campus do Pici S\/N, Bloco 871, 60.440-970, Fortaleza, Ceara, Brazil"}]},{"given":"Edson H.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Laboratorio de Biotecnologia Marinha (BioMar-Lab), Departamento de Engenharia de Pesca, Universidade Federal do Ceara, Campus do Pici S\/N, Bloco 871, 60.440-970, Fortaleza, Ceara, Brazil"}]},{"given":"Paula A.","family":"Videira","sequence":"additional","affiliation":[{"name":"UCIBIO, Departamento de Ciencias da Vida, Faculdade de Ciencias e Tecnologia, Universidade NOVA de Lisboa, 2829-516, Caparica, Portugal"}]}],"member":"965","container-title":["Anti-Cancer Agents in Medicinal Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/eurekaselect.com\/article\/download\/157130","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.eurekaselect.com\/157130\/article","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/eurekaselect.com\/article\/download\/157130","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,26]],"date-time":"2020-12-26T09:45:37Z","timestamp":1608975937000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/157130\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,17]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,7,17]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/1871520617666171114094847","relation":{},"ISSN":["1871-5206"],"issn-type":[{"value":"1871-5206","type":"print"}],"subject":[],"published":{"date-parts":[[2018,7,17]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2017-01-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-08-07","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}