{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T17:36:47Z","timestamp":1772213807916,"version":"3.50.1"},"reference-count":15,"publisher":"Bentham Science Publishers Ltd.","issue":"1","license":[{"start":{"date-parts":[[2017,9,14]],"date-time":"2017-09-14T00:00:00Z","timestamp":1505347200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"content-domain":{"domain":["openbiotechnologyjournal.com","benthamopen.com"],"crossmark-restriction":true},"short-container-title":["TOBIOTJ"],"published-print":{"date-parts":[[2017,9,14]]},"abstract":"\n Objective:<\/jats:title>\n An instrument-free assay was developed for simultaneous detection of laccase activity in a large number of samples as diverse as screening of laccase-producing microbial cultures or chromatographic fractions.<\/jats:p>\n <\/jats:sec>\n \n Method:<\/jats:title>\n Dried paper discs previously impregnated with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) were placed on a flat-bottom microplate (a simple way to avoid misidentification) and loaded with an aliquot from each sample.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n Discs corresponding to samples containing laccase activity become green-bluish colored within first ten minutes of reaction, allowing direct detection through simple naked-eye inspection.<\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n As an example, this easy process was applied to the laccase purification in order to eliminate chromatographic fractions that did not contain laccase activity, thus reducing the number of spectrophotometric assays.<\/jats:p>\n <\/jats:sec>","DOI":"10.2174\/1874070701711010089","type":"journal-article","created":{"date-parts":[[2017,9,21]],"date-time":"2017-09-21T06:36:38Z","timestamp":1505975798000},"page":"89-93","update-policy":"https:\/\/doi.org\/10.2174\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["An Easy Method for Screening and Detection of Laccase Activity"],"prefix":"10.2174","volume":"11","author":[{"given":"Albino A.","family":"Dias","sequence":"first","affiliation":[]},{"given":"Ant\u00f3nio J.S.","family":"Matos","sequence":"additional","affiliation":[]},{"given":"Irene","family":"Fraga","sequence":"additional","affiliation":[]},{"given":"Ana","family":"Sampaio","sequence":"additional","affiliation":[]},{"given":"Rui M.F.","family":"Bezerra","sequence":"additional","affiliation":[]}],"member":"965","reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1007\/s00018-014-1822-x","volume":"72","author":"Martins L.O.","year":"2015","unstructured":"Martins LO, Dur\u00e3o P, Brissos V, Lindley PF. \n Laccases of prokaryotic origin: enzymes at the interface of protein science and protein technology. \n Cell Mol Life Sci \n 2015; \n 72\n (5)\n : 911-22.","journal-title":"Cell. Mol. Life Sci."},{"key":"ref2","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1007\/s00018-009-0169-1","volume":"67","author":"Giardina P.","year":"2010","unstructured":"Giardina P, Faraco V, Pezzella C, Piscitelli A, Vanhulle S, Sannia G. \n Laccases: a never-ending story. \n Cell Mol Life Sci \n 2010; \n 67\n (3)\n : 369-85.","journal-title":"Cell. Mol. Life Sci."},{"key":"ref3","doi-asserted-by":"publisher","first-page":"221","DOI":"10.2174\/1874070701509010221","volume":"9","author":"Zhao L.","year":"2015","unstructured":"Zhao L, Yin W, Wang L. \n High-yield laccase-producing strains constructed by protoplast fusion between bacterium and fungus. \n Open Biotechnol J \n 2015; \n 9\n : 221-4.","journal-title":"Open Biotechnol. J."},{"key":"ref4","doi-asserted-by":"publisher","first-page":"2331","DOI":"10.1016\/j.biortech.2009.10.087","volume":"101","author":"Majeau J.A.","year":"2010","unstructured":"Majeau JA, Brar SK, Tyagi RD. \n Laccases for removal of recalcitrant and emerging pollutants. \n Bioresour Technol \n 2010; \n 101\n (7)\n : 2331-50.","journal-title":"Bioresour. Technol."},{"key":"ref5","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1080\/10643380902945706","volume":"41","author":"Strong P.J.","year":"2011","unstructured":"Strong PJ, Claus H. \n Laccase: a review of its past and its future in bioremediation. \n Crit Rev Environ Sci Technol \n 2011; \n 41\n : 373-434.","journal-title":"Crit. Rev. Environ. Sci. Technol."},{"key":"ref6","doi-asserted-by":"publisher","first-page":"1760","DOI":"10.1007\/s12010-009-8676-y","volume":"160","author":"Singh A. D.","year":"2010","unstructured":"Singh A. D, Kumar S. R. \n Ligninolytic fungal laccases and their biotechnological applications. \n Appl Biochem Biotechnol \n 2010; \n 160\n (6)\n : 1760-88.","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref7","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1080\/10826068.2014.887581","volume":"45","author":"Singha S.","year":"2015","unstructured":"Singha S, Panda T. \n Optimization of laccase fermentation and evaluation of kinetic and thermodynamic parameters of a partially purified laccase produced by Daedalea flavida. \n Prep Biochem Biotechnol \n 2015; \n 45\n (4)\n : 307-35.","journal-title":"Prep. Biochem. Biotechnol."},{"key":"ref8","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1111\/j.1472-765X.2007.02181.x","volume":"45","author":"Matos A.J.","year":"2007","unstructured":"Matos AJ, Bezerra RM, Dias AA. \n Screening of fungal isolates and properties of Ganoderma applanatum intended for olive mill wastewater decolourization and dephenolization. \n Lett Appl Microbiol \n 2007; \n 45\n (3)\n : 270-5.","journal-title":"Lett. Appl. Microbiol."},{"key":"ref9","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.biortech.2003.08.006","volume":"92","author":"Dias A.A.","year":"2004","unstructured":"Dias AA, Bezerra RM, Pereira AN. \n Activity and elution profile of laccase during biological decolorization and dephenolization of olive mill wastewater. \n Bioresour Technol \n 2004; \n 92\n (1)\n : 7-13.","journal-title":"Bioresour. Technol."},{"key":"ref10","first-page":"67","volume":"53","author":"Sundman V.","year":"1971","unstructured":"Sundman V, N\u00e4se L. \n A simple test for direct visualization of biological lignin degradation. \n Paper Timber \n 1971; \n 53\n : 67-71.","journal-title":"Paper Timber"},{"key":"ref11","first-page":"244","volume":"9","author":"Marr C.D.","year":"1979","unstructured":"Marr CD. \n Laccase and tyrosinase oxidation of spot test reagents. \n Mycotaxon \n 1979; \n 9\n : 244-76.","journal-title":"Mycotaxon"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1023\/A:1005680027798","volume":"22","author":"Dekker R.F.","year":"2000","unstructured":"Dekker RF, Ling KY, Barbosa AM. \n A simple method for monitoring chromatography column eluates for laccase activity during enzyme purification. \n Biotechnol Lett \n 2000; \n 22\n : 105-8.","journal-title":"Biotechnol. Lett."},{"key":"ref13","doi-asserted-by":"publisher","first-page":"924","DOI":"10.1074\/jbc.272.2.924","volume":"272","author":"Xu F.","year":"1997","unstructured":"Xu F. \n Effects of redox potential and hydroxide inhibition on the pH activity profile of fungal laccases. \n J Biol Chem \n 1997; \n 272\n (2)\n : 924-8.","journal-title":"J. Biol. Chem."},{"key":"ref14","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1385\/ABAB:98-100:1-9:497","volume":"98-100","author":"Leitner C.","year":"2002","unstructured":"Leitner C, Hess J, Galhaup C, et al. \n Purification and characterization of a laccase from the white-rot fungus Trametes multicolor. \n Appl Biochem Biotechnol \n 2002; \n 98-100\n : 497-507.","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref15","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1134\/S0003683813040169","volume":"49","author":"Kaushik G.","year":"2013","unstructured":"Kaushik G, Thakur IS. \n Purification, characterization and usage of thermotolerant laccase from Bacillus sp. for biodegradation of synthetic dyes. \n Appl Biochem Microbiol \n 2013; \n 49\n : 352-9.","journal-title":"Appl. Biochem. Microbiol."}],"container-title":["The Open Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/openbiotechnologyjournal.com\/contents\/volumes\/V11\/TOBIOTJ-11-89\/TOBIOTJ-11-89.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/openbiotechnologyjournal.com\/contents\/volumes\/V11\/TOBIOTJ-11-89\/TOBIOTJ-11-89.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/openbiotechnologyjournal.com\/contents\/volumes\/V11\/TOBIOTJ-11-89\/TOBIOTJ-11-89.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,9]],"date-time":"2019-04-09T11:55:48Z","timestamp":1554810948000},"score":1,"resource":{"primary":{"URL":"https:\/\/openbiotechnologyjournal.com\/VOLUME\/11\/PAGE\/89\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,14]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,9,14]]}},"alternative-id":["TOBIOTJ-11-89"],"URL":"https:\/\/doi.org\/10.2174\/1874070701711010089","relation":{},"ISSN":["1874-0707"],"issn-type":[{"value":"1874-0707","type":"print"}],"subject":[],"published":{"date-parts":[[2017,9,14]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2017-03-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-07-21","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-08-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-09-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}