{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:52:05Z","timestamp":1773661925836,"version":"3.50.1"},"reference-count":0,"publisher":"Bentham Science Publishers Ltd.","issue":"1","license":[{"start":{"date-parts":[[2015,4,29]],"date-time":"2015-04-29T00:00:00Z","timestamp":1430265600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode"}],"content-domain":{"domain":["openhypertensionjournal.com","benthamopen.com"],"crossmark-restriction":true},"short-container-title":["TOHYPERJ"],"published-print":{"date-parts":[[2015,4,29]]},"abstract":"<jats:p>Renalase was recently described as a new flavoprotein that functions as FAD\/NADH-dependent oxidase and, in\ncontrast to other monoamine oxidases, is secreted into plasma and urine. Recombinant renalase was found to exert powerful\nand rapid hypotensive effects when administered intravenously on rats and this was suggested to be mediated by circulating\ncatecholamines degradation. However there is no concrete evidence that directly supports the hypothesis that\nrenalase metabolizes catecholamines. In this study we aimed to evaluate the catecholamines-degrading renalase activity\nby three different technical approaches: 1) Amplex Red Monoamine Oxidase Assay, which evaluates the rate of resaruzin\nreduction by renalase oxidative activity; 2) assessment of catecholamines consumptions by high-pressure liquid chromatography\n(HPLC) with electrochemical-detection (ED) and 3) assessment of product formation by HPLC with photodiode\narray-detection (DAD). Using the Amplex Red MAO Assay, all three catecholamines were degraded by recombinant\nrenalase overtime, being adrenaline the preferred substrate, followed by noradrenaline and dopamine. In addition using\nHPLC-ED, it was observed the consumption of all three catecholamines by recombinant renalase, which were oxidized to\nthe correspondent aminochromes, as observed by DAD. However the role of renalase as catalyzer of aminochromes production\nis still undefined. In summary, the data presented in this study propose by different methodologies the involvement\nof renalase in catecholamine metabolization.<\/jats:p>","DOI":"10.2174\/1876526201507010014","type":"journal-article","created":{"date-parts":[[2015,4,30]],"date-time":"2015-04-30T11:55:36Z","timestamp":1430394936000},"page":"14-18","update-policy":"https:\/\/doi.org\/10.2174\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Assessment of Renalase Activity on Catecholamines Degradation"],"prefix":"10.2174","volume":"7","author":[{"given":"Janete","family":"Quelhas-Santos","sequence":"first","affiliation":[]},{"given":"Benedita","family":"Sampaio-Maia","sequence":"additional","affiliation":[]},{"given":"Fernando","family":"Remi\u00e3o","sequence":"additional","affiliation":[]},{"given":"Paula","family":"Serr\u00e3o","sequence":"additional","affiliation":[]},{"given":"Isabel","family":"Soares-Silva","sequence":"additional","affiliation":[]},{"given":"Gary V.","family":"Desir","sequence":"additional","affiliation":[]},{"given":"Manuel","family":"Pestana","sequence":"additional","affiliation":[]}],"member":"965","container-title":["The Open Hypertension Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/openhypertensionjournal.com\/contents\/volumes\/V7\/TOHYPERJ-7-14\/TOHYPERJ-7-14.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/openhypertensionjournal.com\/contents\/volumes\/V7\/TOHYPERJ-7-14\/TOHYPERJ-7-14.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/openhypertensionjournal.com\/contents\/volumes\/V7\/TOHYPERJ-7-14\/TOHYPERJ-7-14.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,25]],"date-time":"2019-04-25T11:18:40Z","timestamp":1556191120000},"score":1,"resource":{"primary":{"URL":"https:\/\/openhypertensionjournal.com\/VOLUME\/7\/PAGE\/14\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,29]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,4,29]]}},"alternative-id":["TOHYPERJ-7-14"],"URL":"https:\/\/doi.org\/10.2174\/1876526201507010014","relation":{},"ISSN":["1876-5262"],"issn-type":[{"value":"1876-5262","type":"print"}],"subject":[],"published":{"date-parts":[[2015,4,29]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2014-12-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-02-15","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-02-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-4-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}