{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T20:01:32Z","timestamp":1775937692339,"version":"3.50.1"},"reference-count":50,"publisher":"Bentham Science Publishers Ltd.","issue":"1","funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico, CNPq","doi-asserted-by":"publisher","award":["145\/2012"],"award-info":[{"award-number":["145\/2012"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia\/MCTES, Portugal","doi-asserted-by":"publisher","award":["PTDC\/QEQ-FTT\/1686\/2012"],"award-info":[{"award-number":["PTDC\/QEQ-FTT\/1686\/2012"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia\/MCTES, Portugal","doi-asserted-by":"publisher","award":["UID\/QUI\/00100\/ 2013"],"award-info":[{"award-number":["UID\/QUI\/00100\/ 2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["eurekaselect.com"],"crossmark-restriction":true},"short-container-title":["CBE"],"published-print":{"date-parts":[[2019,9,27]]},"abstract":"<jats:sec>\n<jats:title>Background:<\/jats:title>\n<jats:p>Eutectic solvents are a mixture of two compounds which possess a lower\nmelting temperature than the parent compounds, using quaternary ammonium salts, such as choline\nchloride and betaine hydrochloride and organic acids, polyols and amides as hydrogen bond donors.\nThese solvents can be an alternative as non-aqueous media for enzymatic reactions, mainly using lipases.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Objective:<\/jats:title>\n<jats:p>The objective of this work is to evaluate enzymatic activity and stability of commercial lipases,\nimmobilized or at free form (Thermomyces lanuginosus: Lipozyme TL IM, iTL and Lipolase\n100 L, fTL; Candida antarctica: Novozym 435, iCALB; Novozym 735, iCALA and Novozym CALB\nL, fCALB); and a phospholipase (Lecitase Ultra), in the presence of eutectic solvents (choline chloride\nChCl:urea, ChCl:glycerol, betaine hydrochloride (BeHCl):urea and BeHCl: glycerol.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods:<\/jats:title>\n<jats:p> Initially, lipases were maintained for 2 hours in solutions of choline and betaine-based\neutectic solvents (1 to 20% m\/m) at 25\u00baC compared with water for relative enzymatic activity. Using\nthe solvent that best promoted lipase activity, some parameters were evaluated such as the molar ratio\nbetween quaternary ammonium salts and urea, stocking temperature and kinetics.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results and Conclusion:<\/jats:title>\n<jats:p>These eutectic solvents enable, mainly with immobilized lipases, 25 to 125\ntimes more activity than water at 25\u00baC and 2h, and even after 24h, lipase iTLL was still 40 times more\nactive in the presence of ChCl:Urea 1:3. Lipase iCALB showed great thermostability 47 times higher\nat 55\u00baC, almost double relative activity at 25\u00baC in the presence of BetHCl:Urea 1:4.<\/jats:p>\n<\/jats:sec>","DOI":"10.2174\/2212711906666190710181629","type":"journal-article","created":{"date-parts":[[2019,7,11]],"date-time":"2019-07-11T05:57:30Z","timestamp":1562824650000},"page":"57-68","update-policy":"https:\/\/doi.org\/10.2174\/bsp_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Influence of Betaine- and Choline-based Eutectic Solvents on Lipase Activity"],"prefix":"10.2174","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8621-156X","authenticated-orcid":true,"given":"Bernardo Dias","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Escola de Quimica, Universidade Federal do Rio de Janeiro, 21941-598, Rio de Janeiro, RJ, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4045-7270","authenticated-orcid":true,"given":"Lucas","family":"de Carvalho Iff","sequence":"additional","affiliation":[{"name":"Escola de Quimica, Universidade Federal do Rio de Janeiro, 21941-598, Rio de Janeiro, RJ, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0814-3626","authenticated-orcid":true,"given":"Maria Alice Zarur","family":"Coelho","sequence":"additional","affiliation":[{"name":"Escola de Quimica, Universidade Federal do Rio de Janeiro, 21941-598, Rio de Janeiro, RJ, Brazil"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8733-1958","authenticated-orcid":true,"given":"Isabel M.","family":"Marrucho","sequence":"additional","affiliation":[{"name":"Instituto Superior Tecnico, Universidade de Lisboa, Av. Rodovisco Pais, 1, 1049-001, Lisboa, Portugal"}]}],"member":"965","reference":[{"key":"ref=1","doi-asserted-by":"publisher","first-page":"1513","DOI":"10.3390\/nu10101513","volume":"10","author":"Wiedeman A.M.","year":"2018","unstructured":"Wiedeman A.M.; Barr S.I.; Green T.J.; Xu Z.; Innis S.M.; Kitts D.D.; Dietary choline intake: Current state of knowledge across the life cycle. Nutrients 2018,10(10),1513","journal-title":"Nutrients"},{"key":"ref=2","author":"Abbott A.P.","unstructured":"Abbott A.P.; Capper G.; Davies D.L.; Rasheed R.K.; Tambyrajah V.;  Tambyrajah, Novel solvent properties of choline chloride\/urea mixtures pp. 70-71, 2003.","journal-title":"Tambyrajah, Novel solvent properties of choline chloride\/urea mixtures"},{"key":"ref=3","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.cogsc.2018.12.003","volume":"18","author":"Florindo C.","year":"2019","unstructured":"Florindo C.; Lima F.; Ribeiro B.D.; Marrucho I.M.; Deep Eutectic Solvents: overcoming 21 century challenges. Cur-rent Opinion in Green and Sustainable Chemistry 2019,18,31-36","journal-title":"Cur-rent Opinion in Green and Sustainable Chemistry"},{"key":"ref=4","doi-asserted-by":"publisher","first-page":"11060","DOI":"10.1021\/cr300162p","volume":"114","author":"Smith E.L.","year":"2014","unstructured":"Smith E.L.; Abbott A.P.; Ryder K.S.; Deep eutectic solvents (DESs) and their applications. Chem Rev 2014,114(21),11060-11082","journal-title":"Chem Rev"},{"key":"ref=5","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1016\/j.trac.2015.11.006","volume":"76","author":"Espino M.","year":"2016","unstructured":"Espino M.; Fernandez M.A.; Gomez F.J.V.; Silva M.F.; Natural designer solvents for greening analytical chemistry. Trends Analyt Chem 2016,76,126-136","journal-title":"Trends Analyt Chem"},{"key":"ref=6","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1016\/j.molliq.2015.12.015","volume":"215","author":"Khandelwal S.","year":"2016","unstructured":"Khandelwal S.; Tailor Y.K.; Kumar M.; Deep eutectic solvents (DESs) as eco-friendly and sustainable sol-vent\/catalyst systems in organic transformations. J Mol Liq 2016,215,345-386","journal-title":"J Mol Liq"},{"key":"ref=7","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.foodchem.2015.03.123","volume":"187","author":"Dai Y.","year":"2015","unstructured":"Dai Y.; Witkamp G-J.; Verpoorte R.; Choi Y.H.; Tailoring properties of natural deep eutectic solvents with water to facilitate their applications. Food Chem 2015,187,14-19","journal-title":"Food Chem"},{"key":"ref=8","doi-asserted-by":"publisher","first-page":"7108","DOI":"10.1039\/c2cs35178a","volume":"41","author":"Zhang Q.","year":"2012","unstructured":"Zhang Q.; De Oliveira Vigier K.; Royer S.; J\u00e9r\u00f4me F.; Deep eutectic solvents: syntheses, properties and applications. Chem Soc Rev 2012,41(21),7108-7146","journal-title":"Chem Soc Rev"},{"key":"ref=9","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.biochi.2015.09.019","volume":"120","author":"Durand E.","year":"2016","unstructured":"Durand E.; Lecomte J.; Villeneuve P.; From green chemistry to nature: The versatile role of low transition temperature mixtures. Biochimie 2016,120,119-123","journal-title":"Biochimie"},{"key":"ref=10","doi-asserted-by":"publisher","first-page":"3074","DOI":"10.1002\/anie.201207548","volume":"52","author":"Francisco M.","year":"2013","unstructured":"Francisco M.; van den Bruinhorst A.; Kroon M.C.; Low-transition-temperature mixtures (LTTMs): a new generation of designer solvents. Angew Chem Int Ed Engl 2013,52(11),3074-3085","journal-title":"Angew Chem Int Ed Engl"},{"key":"ref=11","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1039\/C1GC16236E","volume":"14","author":"Vigier K.O.","year":"2012","unstructured":"Vigier K.O.; Benguerba A.; Barrault J.; J\u00e9r\u00f4me F.; Con-version of fructose and inulin to 5-hydroxymethylfurfural in sustainable betaine hydrochloride-based media. Green Chem 2012,14,285-289","journal-title":"Green Chem"},{"key":"ref=12","doi-asserted-by":"publisher","first-page":"18149","DOI":"10.1039\/c3ra43404d","volume":"3","author":"Sharma M.","year":"2013","unstructured":"Sharma M.; Mukesh C.; Mondal D.; Prasad K.; Dissolu-tion of \u03b1-chitin in deep eutectic solvents. RSC Advances 2013,3,18149-18155","journal-title":"RSC Advances"},{"key":"ref=13","doi-asserted-by":"publisher","first-page":"55990","DOI":"10.1039\/C4RA10628H","volume":"4","author":"Cardellini F.","year":"2014","unstructured":"Cardellini F.; Tiecco M.; Germani R.; Cardinali G.; Corte L.; Roscini L.; Spreti N.; Novel zwitterionic deep eutectic solvents from trimethylglycine and carboxylic acids: charac-terization of their properties and their toxicity. RSC Advances 2014,4,55990-56002","journal-title":"RSC Advances"},{"key":"ref=14","doi-asserted-by":"publisher","first-page":"9265","DOI":"10.1007\/s11356-015-4780-4","volume":"23","author":"Kumar A.K.","year":"2016","unstructured":"Kumar A.K.; Parikh B.S.; Pravakar M.; Natural deep eutectic solvent mediated pretreatment of rice straw: bioanalytical characterization of lignin extract and enzymatic hydrolysis of pretreated biomass residue. Environ Sci Pollut Res Int 2016,23(10),9265-9275","journal-title":"Environ Sci Pollut Res Int"},{"key":"ref=15","doi-asserted-by":"publisher","first-page":"2458","DOI":"10.1016\/S1003-6326(15)63862-6","volume":"25","author":"Gong K.","year":"2015","unstructured":"Gong K.; Hua Y-X.; Xu C-Y.; Zhang Q-B.; Li Y.; Ru J-J.; Jie Y-F.; Electrodeposition behavior of bright nickel in air and water-stable betaine\u2022HCl_ethylene glycol ionic liquid. Trans Nonferrous Met Soc China 2015,25,2458-2465","journal-title":"Trans Nonferrous Met Soc China"},{"key":"ref=16","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.indcrop.2015.03.026","volume":"70","author":"Qi X-L.","year":"2015","unstructured":"Qi X-L.; Peng X.; Huang Y-Y.; Li L.; Wei Z-F.; Zu Y-G.; Fu Y-J.; Green and efficient extraction of bioactive flavo-noids from Equisetum palustre L. by deep eutectic solvents-based negative pressure cavitation method combined with macroporous resin enrichment. Ind Crops Prod 2015,70,142-148","journal-title":"Ind Crops Prod"},{"key":"ref=17","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.talanta.2016.01.042","volume":"152","author":"Li N.","year":"2016","unstructured":"Li N.; Wang Y.; Xu K.; Huang Y.; Wen Q.; Ding X.; Development of green betaine-based deep eutectic solvent aqueous two-phase system for the extraction of protein. Talanta 2016,152,23-32","journal-title":"Talanta"},{"key":"ref=18","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1186\/s40643-017-0165-5","volume":"4","author":"Xu P.","year":"2017","unstructured":"Xu P.; Zheng G-W.; Zong M-H.; Li N.; Lou W-Y.; Re-cent progress on deep eutectic solvents in biocatalysis, Eur. J. Lipid Sci. Technol. 115 (2013) 379\u2013385. Bioresour Bioprocess 2017,4,34","journal-title":"Bioresour Bioprocess"},{"key":"ref=19","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.procbio.2017.12.003","volume":"66","author":"Juneidi I.","year":"2018","unstructured":"Juneidi I.; Hayyan M.; Hashim M.A.; Intensification of biotransformations using deep eutectic solvents: Overview and outlook. Process Biochem 2018,66,33-60","journal-title":"Process Biochem"},{"key":"ref=20","doi-asserted-by":"publisher","first-page":"2275","DOI":"10.1039\/c3gc40899j","volume":"15","author":"Durand E.","year":"2013","unstructured":"Durand E.; Lecomte J.; Bar\u00e9a B.; Dubreucq E.; Lortie R.; Villeneuve P.; Evaluation of deep eutectic solvent\u2013water bi-nary mixtures for lipase-catalyzed lipophilization of phenolic acids. Green Chem 2013,15,2275-2282","journal-title":"Green Chem"},{"key":"ref=21","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1002\/ejlt.201400459","volume":"117","author":"P\u00f6hnlein M.","year":"2015","unstructured":"P\u00f6hnlein M.; Ulrich J.; Kirschh\u00f6fer F.; Nusser M.; Muhle-Goll C.; Kannengiesser B.; Brenner-Weiss G.; Luy B.; Liese A.; Syldatk C.; Hausmann R.; Lipase-catalyzed synthesis of glucose-6-O-hexanoate in deep eutectic solvents. Eur J Lipid Sci Technol 2015,117,161-166","journal-title":"Eur J Lipid Sci Technol"},{"key":"ref=22","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1016\/j.molcatb.2015.09.005","volume":"122","author":"Bubalo M.C.","year":"2015","unstructured":"Bubalo M.C.; Tusek A.J.; Vinkovic M.; Radosevic K.; Srcek V.G.; Redovnikovic I.R.; Cholinium-based deep eutec-tic solvents and ionic liquids for lipase-catalyzed synthesis of butyl acetate. J Mol Catal, B Enzym 2015,122,188-198","journal-title":"J Mol Catal, B Enzym"},{"key":"ref=23","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1002\/ejlt.201400494","volume":"117","author":"Kleiner B.","year":"2014","unstructured":"Kleiner B.; Sch\u00f6rken U.; Native lipase dissolved in hy-drophilic green solvents: A versatile 2-phase reaction system for high yield ester synthesis. Eur J Lipid Sci Technol 2014,117,167-177","journal-title":"Eur J Lipid Sci Technol"},{"key":"ref=24","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1016\/j.jbiotec.2005.11.006","volume":"123","author":"Kim H.J.","year":"2006","unstructured":"Kim H.J.; Youn S.H.; Shin C.S.; Lipase-catalyzed synthesis of sorbitol-fatty acid esters at extremely high substrate concentrations. J Biotechnol 2006,123(2),174-184","journal-title":"J Biotechnol"},{"key":"ref=25","doi-asserted-by":"publisher","first-page":"2053","DOI":"10.1007\/s00449-015-1445-0","volume":"38","author":"Zeng C-X.","year":"2015","unstructured":"Zeng C-X.; Qi S-J.; Xin R-P.; Yang B.; Wang Y-H.; Enzymatic selective synthesis of 1,3-DAG based on deep eutectic solvent acting as substrate and solvent. Bioprocess Biosyst Eng 2015,38(11),2053-2061","journal-title":"Bioprocess Biosyst Eng"},{"key":"ref=26","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.molcatb.2015.10.013","volume":"123","author":"Zhang Y.","year":"2016","unstructured":"Zhang Y.; Xia X.; Duan M.; Han Y.; Liu J.; Luo M.; Zhao C.; Zu Y.; Fu Y.; Green deep eutectic solvent assisted en-zymatic preparation of biodiesel from yellow horn seed oil with microwave irradiation. J Mol Catal, B Enzym 2016,123,35-40","journal-title":"J Mol Catal, B Enzym"},{"key":"ref=27","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1016\/j.molcatb.2012.09.003","volume":"85-86","author":"Zhao H.","year":"2013","unstructured":"Zhao H.; Zhang C.; Crittle T.D.; Choline-based deep eutectic solvents for enzymatic preparation of biodiesel from soybean oil. J Mol Catal, B Enzym 2013,85-86,243-247","journal-title":"J Mol Catal, B Enzym"},{"key":"ref=28","doi-asserted-by":"publisher","first-page":"1513","DOI":"10.1002\/ejoc.201501553","author":"Gonz\u00e1lez-Mart\u00ednez D.","year":"2016","unstructured":"Gonz\u00e1lez-Mart\u00ednez D.; Gotor V.; Gotor-Fernandez V.; Application of Deep Eutectic Solvents in Promiscuous Li-pase-Catalysed Aldol Reactions. Eur J Org Chem 2016,1513-1519","journal-title":"Eur J Org Chem"},{"key":"ref=29","doi-asserted-by":"publisher","first-page":"80","DOI":"10.4014\/jmb.1506.06075","volume":"26","author":"Tian X.","year":"2016","unstructured":"Tian X.; Zhang S.; Zheng L.; Enzyme-Catalyzed Henry Reaction in Choline Chloride-Based Deep Eutectic Solvents. J Microbiol Biotechnol 2016,26(1),80-88","journal-title":"J Microbiol Biotechnol"},{"key":"ref=30","author":"Ribeiro B.D.","unstructured":"Ribeiro B.D.; Castro A.M.; Coelho M.A.Z.; Freire D.M.G.;  Freire, Production and Use of Lipases in Bioenergy: A Review from the Feedstocks to Biodiesel Production, Enzyme Res Article ID 615803 (2011) 16 pages","journal-title":"Freire, Production and Use of Lipases in Bioenergy: A Review from the Feedstocks to Biodiesel Production, Enzyme Res Article ID 615803 (2011) 16 pages"},{"key":"ref=31","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1021\/op0200165","volume":"6","author":"Kirk O.","year":"2002","unstructured":"Kirk O.; Christensen M.W.; Lipases from Candida ant-arctica: Unique Biocatalysts from a Unique Origin. Org Process Res Dev 2002,6,446-451","journal-title":"Org Process Res Dev"},{"key":"ref=32","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.molcatb.2005.09.001","volume":"37","author":"de Mar\u00eda P.D.","year":"2005","unstructured":"de Mar\u00eda P.D.; Carboni-Oerlemans C.; Tuin B.; Bargeman G.; van der Meer A.; van Gemert R.; Biotechnological appli-cations of Candida antarctica lipase A: State-of-the-art. J Mol Catal, B Enzym 2005,37,36-46","journal-title":"J Mol Catal, B Enzym"},{"key":"ref=33","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1007\/s11746-005-1131-0","volume":"82","author":"Song J.K.","year":"2005","unstructured":"Song J.K.; Han J.H.; Rhee J.S.; Phospholipases: Occur-rence and Production in Microorganisms, Assay for High-Throughput Screening, and Gene Discovery from Natural and Man-Made Diversity. J Am Oil Chem Soc 2005,82,691-705","journal-title":"J Am Oil Chem Soc"},{"key":"ref=34","doi-asserted-by":"publisher","first-page":"1368","DOI":"10.1002\/jctb.1478","volume":"81","author":"Amaral P.F.F.","year":"2006","unstructured":"Amaral P.F.F.; Rocha-Le\u00e3o M.H.M.; Marrucho I.M.; Coutinho J.A.P.; Coelho M.A.Z.; Improving lipase production using a perfluorocarbon as oxygen carrier. J Chem Technol Biotechnol 2006,81,1368-1374","journal-title":"J Chem Technol Biotechnol"},{"key":"ref=35","doi-asserted-by":"publisher","first-page":"868","DOI":"10.1039\/b702833d","volume":"9","author":"Abbott A.P.","year":"2007","unstructured":"Abbott A.P.; Cullis P.M.; Gibson M.J.; Harris R.C.; Raven E.; Extraction of glycerol from biodiesel into a eutectic based ionic liquid. Green Chem 2007,9,868-872","journal-title":"Green Chem"},{"key":"ref=36","doi-asserted-by":"publisher","first-page":"12361","DOI":"10.1021\/acs.jafc.8b04804","volume":"66","author":"Liang H.","year":"2018","unstructured":"Liang H.; Qin X.; Tan C.P.; Li D.; Wang Y.; Choline chloride-based eutectic solvent for the efficient production of docosahexaenoyl ethanolamide and eicosapentaenoyl ethano-lamide via an enzymatic process. J Agric Food Chem 2018,66(46),12361-12367","journal-title":"J Agric Food Chem"},{"key":"ref=37","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/j.molcatb.2009.11.010","volume":"62","author":"Fernandez-Lafuente R.","year":"2010","unstructured":"Fernandez-Lafuente R.; Lipase from Thermomyces lanugi-nosus: Uses and prospects as an industrial biocatalyst. J Mol Catal, B Enzym 2010,62,197-212","journal-title":"J Mol Catal, B Enzym"},{"key":"ref=38","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/S0927-7765(02)00032-2","volume":"26","author":"S\u00f6derlund T.","year":"2002","unstructured":"S\u00f6derlund T.; Zhu K.; Jutila A.; Kinnunen P.K.J.; Effects of betaine on the structural dynamics of Thermomyces (Hu-micola) lanuginosa lipase. Colloids Surf B Biointerfaces 2002,26,75-83","journal-title":"Colloids Surf B Biointerfaces"},{"key":"ref=39","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1002\/ejlt.201300246","volume":"116","author":"Durand E.","year":"2014","unstructured":"Durand E.; Lecomte J.; Bar\u00e9a B.; Villeneuve P.; Towards a better understanding of how to improve lipase catalyzed re-actions using deep eutectic solvents based on choline chlo-ride. Eur J Lipid Sci Technol 2014,116,16-23","journal-title":"Eur J Lipid Sci Technol"},{"key":"ref=40","doi-asserted-by":"publisher","first-page":"14882","DOI":"10.1039\/c4cp00503a","volume":"16","author":"Monhemi H.","year":"2014","unstructured":"Monhemi H.; Housaindokht M.R.; Moosavi-Movahedi A.A.; Bozorgmehr M.R.; How a protein can remain stable in a solvent with high content of urea: insights from molecular dynamics simulation of Candida antarctica lipase B in urea\u2009:\u2009choline chloride deep eutectic solvent. Phys Chem Chem Phys 2014,16(28),14882-14893","journal-title":"Phys Chem Chem Phys"},{"key":"ref=41","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1016\/j.molcatb.2011.06.008","volume":"72","author":"Xu Y.","year":"2011","unstructured":"Xu Y.; Nordblad M.; Nielsen P.M.; Brask J.; Wood-ley J.M.; In situ visualization and effect of glycerol in lipase-catalyzed ethanolysis of rapeseed oil. J Mol Catal, B Enzym 2011,72,213-219","journal-title":"J Mol Catal, B Enzym"},{"key":"ref=42","doi-asserted-by":"publisher","first-page":"1235","DOI":"10.1039\/b716317g","author":"Gorke J.T.","year":"2008","unstructured":"Gorke J.T.; Srienc F.; Kazlauskas R.J.; Hydrolase-catalyzed biotransformations in deep eutectic solvents. Chem Commun (Camb) 2008(10),1235-1237","journal-title":"Chem Commun (Camb)"},{"key":"ref=43","doi-asserted-by":"publisher","first-page":"20774","DOI":"10.3390\/ijms160920774","volume":"16","author":"Borrelli G.M.","year":"2015","unstructured":"Borrelli G.M.; Trono D.; Recombinant lipases and phospholipases and their use as biocatalysts for industrial applications. Int J Mol Sci 2015,16(9),20774-20840","journal-title":"Int J Mol Sci"},{"key":"ref=44","doi-asserted-by":"publisher","first-page":"2854","DOI":"10.1016\/j.tetasy.2009.11.012","volume":"20","author":"Mishra M.K.","year":"2009","unstructured":"Mishra M.K.; Kumaraguru T.; Sheelu G.; Fadnavis N.W.; Lipase activity of Lecitase_ Ultra: characterization and appli-cations in enantioselective reactions. Tetrahedron Asymmetry 2009,20,2854-2860","journal-title":"Tetrahedron Asymmetry"},{"key":"ref=45","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1016\/j.foodhyd.2010.03.010","volume":"24","author":"Hu M.","year":"2010","unstructured":"Hu M.; Li Y.; Decker E.A.; McClements D.J.; Role of calcium and calcium-binding agents on the lipase digestibility of emulsified lipids using an in vitro digestion model. Food Hydrocoll 2010,24,719-725","journal-title":"Food Hydrocoll"},{"key":"ref=46","doi-asserted-by":"publisher","first-page":"359","DOI":"10.15255\/CABEQ.2018.1335","volume":"32","author":"Elgharbawy A.A.","year":"2018","unstructured":"Elgharbawy A.A.; Hayyan A.; Hayyan M.; Rashid S.N.; Nor M.R.M.; Zulkifli M.Y.; Alias Y.; Mirghani M.E.S.; Shedding Light on Lipase Stability in Natural Deep Eutectic Solvents. Chem Biochem Eng Q 2018,32,359-370","journal-title":"Chem Biochem Eng Q"},{"key":"ref=47","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/j.bpc.2010.12.008","volume":"156","author":"Kamal M.Z.","year":"2011","unstructured":"Kamal M.Z.; Ahmad S.; Rao N.M.; Stabilizing effect of polyols is sensitive to inherent stability of protein. Biophys Chem 2011,156(1),68-71","journal-title":"Biophys Chem"},{"key":"ref=48","doi-asserted-by":"publisher","first-page":"1907","DOI":"10.1021\/acs.jafc.6b05372","volume":"65","author":"Andler S.M.","year":"2017","unstructured":"Andler S.M.; Wang L-S.; Rotello V.M.; Goddard J.M.; Influence of hierarchical interfacial assembly on lipase sta-bility and performance in Deep Eutectic Solvent. J Agric Food Chem 2017,65(9),1907-1914","journal-title":"J Agric Food Chem"},{"key":"ref=49","doi-asserted-by":"publisher","first-page":"1908","DOI":"10.1039\/c0ob01011a","volume":"9","author":"Zhao H.","year":"2011","unstructured":"Zhao H.; Baker G.A.; Holmes S.; New eutectic ionic liquids for lipase activation and enzymatic preparation of biodiesel. Org Biomol Chem 2011,9(6),1908-1916","journal-title":"Org Biomol Chem"},{"key":"ref=50","doi-asserted-by":"publisher","first-page":"2081","DOI":"10.1016\/j.procbio.2012.07.027","volume":"47","author":"Durand E.","year":"2012","unstructured":"Durand E.; Lecomte J.; Bar\u00e9a B.; Piombo G.; Dubreucq E.; Villeneuve P.; Evaluation of deep eutectic solvents as new media for Candida antarctica B lipase catalyzed reactions. Process Biochem 2012,47,2081-2089","journal-title":"Process Biochem"}],"container-title":["Current Biochemical Engineering"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/eurekaselect.com\/article\/download\/173400","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,29]],"date-time":"2019-10-29T12:32:04Z","timestamp":1572352324000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.eurekaselect.com\/173400\/article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,27]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,9,27]]}},"alternative-id":["LiveAll1"],"URL":"https:\/\/doi.org\/10.2174\/2212711906666190710181629","relation":{},"ISSN":["2212-7119"],"issn-type":[{"value":"2212-7119","type":"print"}],"subject":[],"published":{"date-parts":[[2019,9,27]]},"assertion":[{"value":"Peer Reviewed","order":0,"name":"review_status","label":"Review Status","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Single blind","order":1,"name":"review_process","label":"Review Process","group":{"name":"peer_review_details","label":"Peer Review Details"}},{"value":"Checked with iThenticate","order":0,"name":"screening_status","label":"Screening Status","group":{"name":"plagiarism_screening","label":"Plagiarism Screening"}},{"value":"2019-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-30","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}