{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,3,20]],"date-time":"2023-03-20T17:12:28Z","timestamp":1679332348510},"reference-count":0,"publisher":"BENTHAM SCIENCE PUBLISHERS","isbn-type":[{"value":"9781608051908","type":"print"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,12,1]]},"abstract":"<jats:p>The metabolome comprises all metabolites in a biological organism, which constitute the end\nproducts of its gene expression. Metabolomics consists on the systematic study of the chemical fingerprints\nresulting from specific cellular processes or, more particularly, the study of an organism\u2019s profile of low\nmolecular weight metabolites. Thus, metabolomics is perhaps the ultimate level of post-genomic analysis as\nit can reveal changes in metabolite fluxes that are controlled by only minor changes within gene expression.\nClassical phytochemical approaches often comprised a rather tedious and time consuming process of\nisolation, dereplication of known substances, followed by structure elucidation and quantification. However,\nit is important to highlight that, in many situations, the effects of natural products are not due to a single\ncompound, but to a mixture of related and unrelated ones. Thus, metabolomics provides an efficient tool for\nthe quality control and authentication of medicines of natural origin, contributing as well to the\ncharacterization of different species. Several combined techniques have been applied in the measurements of\nintracellular metabolites, whether qualitative or quantitative, which reveal the biochemical status of the\norganism. This review offers an insight on the methods used in the metabolomics analysis (LC-MS, GC-MS,\nHPLC-DAD, NMR) of several natural matrices with protective health potential, with special emphasis on the\ndetermination of phenolics profiles, once these represent the most abundant and widely spread class of plant\nnatural compounds, additionally exhibiting interesting biological activities.<\/jats:p>","DOI":"10.2174\/978160805190811001010001","type":"book-chapter","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T08:39:39Z","timestamp":1301647179000},"page":"1-19","source":"Crossref","is-referenced-by-count":0,"title":["Metabolomic Analysis of Natural Products"],"prefix":"10.2174","author":[{"given":"David M. Pereira","family":"M. Pereira","sequence":"first","affiliation":[]},{"given":"Patricia","family":"Valentao","sequence":"additional","affiliation":[]},{"given":"Federico","family":"Ferreres","sequence":"additional","affiliation":[]},{"given":"Paula","family":"B. Andrade","sequence":"additional","affiliation":[{"name":"REQUIMTE\/Servico de Farmacognosia, Faculdade de Farmacia, Universidade do Porto, R. Anibal Cunha,164, 4050-047 Porto, Portugal"}]}],"member":"965","container-title":["Reviews in Pharmaceutical &amp; Biomedical Analysis"],"original-title":[],"deposited":{"date-parts":[[2023,3,20]],"date-time":"2023-03-20T16:48:16Z","timestamp":1679330896000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.eurekaselect.com\/node\/52146"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12,1]]},"ISBN":["9781608051908"],"references-count":0,"URL":"https:\/\/doi.org\/10.2174\/978160805190811001010001","relation":{},"subject":[],"published":{"date-parts":[[2010,12,1]]}}}