{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T07:49:40Z","timestamp":1694850580759},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Water Environment Research"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:p>A laboratory study was performed as the basis for a full\u2010scale bioaugmentation project at a site contaminated with chlorinated ethenes. The objectives of this study were to 1) develop a protocol to enrich for a tetrachloroethene (PCE)\u2010dechlorinating culture from waste activated sludge and anaerobic digester biosolids and 2) monitor the survival of fecal coliform bacteria and bacteriophage, which model enteric viruses, during the enrichment process. A culture was enriched in 8 days with the ability to degrade 6\u2010\u03bcM PCE to <jats:italic>cis<\/jats:italic>\u2010dichloroethene. Using the enrichment protocol in two identical experiments, significant inactivation of fecal coliform bacteria (2 log) and somatic coliphage (0.33 log) was observed in one of the experiments; no inactivation occurred in the second experiment. The number of F\u2010specific coliphage decreased in both experiments (0.87 and 1.26 log inactivation). Despite the decrease in some of the coliform and bacteriophage numbers, the quantity of organisms and phage particles present after enrichment was still high (\u223c7.5 \u00d7 10<jats:sup>5<\/jats:sup> most probable number\/L, 6.9 \u00d7 10<jats:sup>6<\/jats:sup> plaque\u2010forming units (PFU)\/L, and 3.3 \u00d7 10<jats:sup>5<\/jats:sup> PFU\/L, for fecal coliform bacteria, somatic coliphage, and F\u2010specific coliphage, respectively). This may be cause for concern, depending on the current and future groundwater use at or near a site undergoing bioaugmentation with cultures derived from waste activated sludge and anaerobic digester biosolids.<\/jats:p>","DOI":"10.2175\/106143003x141178","type":"journal-article","created":{"date-parts":[[2006,9,16]],"date-time":"2006-09-16T13:25:41Z","timestamp":1158413141000},"page":"368-376","source":"Crossref","is-referenced-by-count":1,"title":["Survival of Indicator Organisms during Enrichment on Tetrachloroethene"],"prefix":"10.1002","volume":"75","author":[{"given":"J. D.","family":"Skramstad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. J.","family":"Hurst","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. J.","family":"Novak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,7]]},"container-title":["Water Environment Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.2175%2F106143003X141178","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.2175\/106143003X141178","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T18:31:31Z","timestamp":1694802691000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.2175\/106143003X141178"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.2175\/106143003X141178"],"URL":"https:\/\/doi.org\/10.2175\/106143003x141178","archive":["Portico"],"relation":{},"ISSN":["1061-4303","1554-7531"],"issn-type":[{"value":"1061-4303","type":"print"},{"value":"1554-7531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7]]}}}