{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T17:02:11Z","timestamp":1782406931558,"version":"3.54.5"},"reference-count":37,"publisher":"Bio-Protocol, LLC","issue":"14","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BIO-PROTOCOL"],"published-print":{"date-parts":[[2020]]},"DOI":"10.21769\/bioprotoc.3689","type":"journal-article","created":{"date-parts":[[2020,7,20]],"date-time":"2020-07-20T06:07:19Z","timestamp":1595225239000},"source":"Crossref","is-referenced-by-count":14,"title":["In vitro Induction and Detection of Acrosomal Exocytosis in Human Spermatozoa"],"prefix":"10.21769","volume":"10","author":[{"given":"Shenae","family":"Cafe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett","family":"Nixon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"9223","reference":[{"key":"ref213257","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a137910"},{"key":"ref213258","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gat067"},{"key":"ref213259","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.34.3.c1"},{"key":"ref313260","unstructured":"Biggers, J. D. (1971). Metabolism of mouse embryos. J Reprod Fertil Suppl 14: 41-54."},{"key":"ref213261","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.072532mb"},{"key":"ref213262","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.114.117911"},{"key":"ref213263","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.21393"},{"key":"ref213264","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21781"},{"key":"ref213265","doi-asserted-by":"publisher","DOI":"10.1007\/BF02765780"},{"key":"ref213266","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-019-0701-1"},{"key":"ref213267","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00073-14"},{"key":"ref213268","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M112.020339"},{"key":"ref213269","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20783"},{"key":"ref213270","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.20787"},{"key":"ref213271","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioy009"},{"key":"ref213272","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-53983-9"},{"key":"ref213273","doi-asserted-by":"publisher","DOI":"10.3109\/01485018108999319"},{"key":"ref213274","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0700219"},{"key":"ref213275","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a019466"},{"key":"ref213276","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2016.05.004"},{"key":"ref213277","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199904)52:4<445::AID-MRD14>3.0.CO;2-6"},{"key":"ref213278","doi-asserted-by":"publisher","DOI":"10.1016\/s0015-0282(16)58459-3"},{"key":"ref213279","doi-asserted-by":"publisher","DOI":"10.1016\/s0015-0282(02)04555-7"},{"key":"ref213280","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/16.6.1185"},{"key":"ref213281","doi-asserted-by":"publisher","DOI":"10.1016\/s0015-0282(03)00603-4"},{"key":"ref213282","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gam043"},{"key":"ref313283","unstructured":"Nixon, B. and Bromfield, E. G. (2018). Sperm capacitation. In: The Encyclopedia of Reproduction. Skinner, M. K. (Ed.). Cambridge, MA, USA, Academic Press: Elsevier. 3: 272-278."},{"key":"ref313284","unstructured":"Nixon, B. and Bromfield, E. G. (2020). New horizons in male subfertility and infertility. In: Male and Sperm Factors that Maximize IVF Success. Aitken, R. J., Mortimer, D. and G. Kovacs (Eds.). UK, Cambridge University Press. 1: 15-27."},{"key":"ref313285","unstructured":"Nixon, B., Cafe, S. L., Bromfield, E. G., De Iuliis, G. N. and Dun, M. D. (2020). Capacitation and acrosome reaction: Histochemical techniques to assess the acrosome reaction. In: Manual of Sperm Function Testing in Human Assisted Reproduction. Agarwal, A., Henkel, R. and Majzoub, A. (Eds.). UK, Cambridge University Press."},{"key":"ref213286","doi-asserted-by":"publisher","DOI":"10.1016\/s0015-0282(16)55270-4"},{"key":"ref213287","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.05.674"},{"key":"ref213288","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.392803"},{"key":"ref213289","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-30567-7_5"},{"key":"ref213290","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0740383"},{"key":"ref213291","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1317113110"},{"key":"ref213292","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1317113110"},{"key":"ref213293","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gax044"}],"container-title":["BIO-PROTOCOL"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/bio-protocol.org\/e3689","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T13:14:22Z","timestamp":1704978862000},"score":1,"resource":{"primary":{"URL":"https:\/\/bio-protocol.org\/e3689"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020]]},"references-count":37,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2020]]}},"alternative-id":["e3689"],"URL":"https:\/\/doi.org\/10.21769\/bioprotoc.3689","relation":{},"ISSN":["2331-8325"],"issn-type":[{"value":"2331-8325","type":"print"}],"subject":[],"published":{"date-parts":[[2020]]}}}