{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T17:17:12Z","timestamp":1762363032827,"version":"build-2065373602"},"reference-count":0,"publisher":"International Institute of Anticancer Research","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CDP"],"published-print":{"date-parts":[[2025,11,1]]},"abstract":"<jats:p>Background\/Aim: Neo-lymphangiogenesis induces lymphatic invasion of cancer cells, significantly increasing the metastatic potential of breast carcinomas (BCs). Among the molecules that are implicated in lymphangiogenesis, podoplanin (PDPN, gene locus: 1p36.21) - a transmembrane receptor glycoprotein - is expressed exclusively in lymphatic vessels. The current study explored the impact of PDPN-dependent mean lymphatic micro-vessel density (mLMVD) in invasive ductal (inDBC) and invasive lobular breast adenocarcinomas (inLBC).\nMaterials and Methods: A set of thirty (n=30) paraffin-embedded invasive BC tissue sections (22 inDBCs and 8 inLBCs, respectively) were analyzed by applying a combination of immunocytochemistry (IHC) and digital image analysis (DIA) assays.\nResults: High and moderate mLMVD rates (defined by the mean number of lymphatic domains with emboli in five optical fields under 400X magnification) were detected in 5\/30 (16.6%) and 6\/30 (20%) cases (total 11\/30 (36.6%), respectively. In these cases, PDPN demonstrated strong cytoplasmic\/membranous staining intensity. The remaining 19 cases (63.4%) demonstrated low levels of mLMVD. mLMVD was significantly correlated with the stage of the examined malignancies (p=0.019), whereas a marginal association with the grade of differentiation was identified (p=0.042). No significant correlation was observed with histological subtype (p=0.234) or tumor size (p=0.085).\nConclusion: Neo-lymphangiogenesis in BCs is a critical histological feature in the progression of the malignancy and is correlated with an aggressive phenotype (advanced stage). PDPN expression in lymphatic micro vessels is a reliable biomarker for evaluating lymphangiogenic activity in BCs, independently of their histotype, especially when assessed with precise DIA techniques.<\/jats:p>","DOI":"10.21873\/cdp.10500","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T17:12:35Z","timestamp":1762362755000},"page":"855-861","source":"Crossref","is-referenced-by-count":0,"title":["Podoplanin \u2013 Related Lymphatic Micro Vessel Density Digital Analysis in Breast Adenocarcinoma"],"prefix":"10.21873","volume":"5","author":[{"given":"GEORGIOS I.","family":"METAXAS","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SPYRIDON","family":"MARINOPOULOS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MARIA","family":"ADAMOPOULOU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KALLIROI","family":"GOULA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"IOANNIS K.","family":"PAPAPANAGIOTOU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"EVANGELOS","family":"TSIAMBAS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SOFIANIKI","family":"MASTRONIKOLI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"EFTYCHIA","family":"PAPACHATZOPOULOU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PANAGIOTIS","family":"FOTIADES","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ATHANASIOS","family":"NIOTIS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GEORGE","family":"TSOUVELAS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SOFIA","family":"KOURA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CONSTANTINE","family":"DIMITRAKAKIS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"9336","published-online":{"date-parts":[[2025,11,1]]},"container-title":["Cancer Diagnosis &amp; Prognosis"],"original-title":[],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T17:12:39Z","timestamp":1762362759000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cancerdiagnosisprognosis.org\/article\/554\/podoplanin-related-lymphatic-micro-vessel-density-digital-analysis-in-breast-adenocarcinoma"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,1]]},"references-count":0,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,11,1]]},"published-print":{"date-parts":[[2025,11,1]]}},"URL":"https:\/\/doi.org\/10.21873\/cdp.10500","relation":{},"ISSN":["2732-7787"],"issn-type":[{"value":"2732-7787","type":"print"}],"subject":[],"published":{"date-parts":[[2025,11,1]]}}}