{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T03:19:53Z","timestamp":1790565593991,"version":"4.1.0"},"reference-count":18,"publisher":"JMIR Publications Inc.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMIR Res Protoc"],"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Interdental spaces are particularly susceptible to biofilm accumulation and gingival inflammation, which contribute to periodontal diseases and their systemic associations. While interdental brushes (IDBs) are recognized as the most effective method of interdental cleaning, their efficacy depends on proper adaptation to the interdental space. Calibration with a colorimetric probe may enhance their effectiveness and comfort. However, evidence directly comparing calibrated and noncalibrated IDBs, especially in young adults, a key target group for preventive strategies, remains limited. The Hygiene of Interdental Junctions in Adults (HIJA) trial was designed to address this gap.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n This protocol aims to compare the clinical, microbiological, and acceptability outcomes associated with calibrated versus noncalibrated IDBs in young adults, focusing on interdental inflammation, periodontal indices, and microbiota composition.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n The HIJA trial is a monocentric, randomized, controlled, single-blind, and parallel-arm study. Overall, 50 healthy, nonsmoking adults aged 18\u201030 years will be randomized (1:1) to receive either calibrated or noncalibrated IDBs. Participants will perform daily interdental cleaning in addition to conventional toothbrushing for 3 months. The primary outcome will be the reduction in interdental inflammation, expressed as the change in bleeding on probing at 3 months. Secondary outcomes will assess differences in interdental microbiota composition (16S ribosomal RNA sequencing), periodontal indices (plaque index, gingival index, probing depth, and clinical attachment loss), and user acceptability measured through the Theoretical Framework of Acceptability questionnaire at 1, 2, and 3 months.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n The HIJA trial will generate evidence on whether calibrated IDBs provide additional benefits over noncalibrated brushes in reducing interdental inflammation and improving oral health in young adults.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n HIJA findings could contribute to the implementation of clinical guidelines and preventive strategies for interdental hygiene in daily practice.<\/jats:p>\n <\/jats:sec>","DOI":"10.2196\/85154","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T08:50:06Z","timestamp":1761555006000},"page":"e85154-e85154","source":"Crossref","is-referenced-by-count":3,"title":["Effectiveness of Different Methods of Interdental Hygiene in Daily Practice Among Young Adults: Protocol for a Randomized, Single-Blind Controlled Trial"],"prefix":"10.2196","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6823-1636","authenticated-orcid":false,"given":"Florence","family":"Carrouel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5612-880X","authenticated-orcid":false,"given":"Romain","family":"Lan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1151-2653","authenticated-orcid":false,"given":"Ina","family":"Saliasi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4754-2512","authenticated-orcid":false,"given":"Denis","family":"Bourgeois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3222-6130","authenticated-orcid":false,"given":"Nicolas","family":"Benech","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8177-7166","authenticated-orcid":false,"given":"Audrey","family":"Murat-Ringot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9936-7691","authenticated-orcid":false,"given":"St\u00e9phane","family":"Viennot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1010","published-online":{"date-parts":[[2025,12,4]]},"reference":[{"issue":"1","key":"R1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-35337-8","article-title":"An umbrella review of the evidence linking oral health and systemic noncommunicable diseases","volume":"13","author":"Botelho","journal-title":"Nat Commun"},{"issue":"10","key":"R2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7100424","article-title":"Periodontal pathogens as risk factors of cardiovascular diseases, diabetes, rheumatoid arthritis, cancer, and chronic obstructive pulmonary disease-is there cause for consideration?","volume":"7","author":"Bourgeois","journal-title":"Microorganisms"},{"key":"R3","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.855668","article-title":"Editorial: the oral microbiome is a key factor in oral and systemic health","volume":"13","author":"Bourgeois","journal-title":"Front Microbiol"},{"issue":"3","key":"R4","doi-asserted-by":"publisher","first-page":"2253","DOI":"10.1007\/s00784-021-04327-3","article-title":"Effectiveness of interdental cleaning devices with active substances: a systematic review","volume":"26","author":"Langa","journal-title":"Clin Oral Investig"},{"issue":"1","key":"R5","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1111\/idh.12492","article-title":"The efficacy of a rubber bristles interdental cleaner on parameters of oral soft tissue health-a systematic review","volume":"20","author":"van der Weijden","journal-title":"Int J Dent Hyg"},{"issue":"6","key":"R6","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1111\/eos.12302","article-title":"Efficacy of interdental calibrated brushes on bleeding reduction in adults: a 3-month randomized controlled clinical trial","volume":"124","author":"Bourgeois","journal-title":"Eur J Oral Sci"},{"issue":"1","key":"R7","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-51938-8","article-title":"Calibrated interdental brushing for the prevention of periodontal pathogens infection in young adults - 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