{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T19:47:43Z","timestamp":1791920863670,"version":"4.3.4"},"reference-count":26,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","funder":[{"DOI":"10.13039\/spdf61vocgkt","name":"Italian Ministry of Health","doi-asserted-by":"publisher","award":["Ricerca Finalizzata 2009 RF\/2009 1471624"],"award-info":[{"award-number":["Ricerca Finalizzata 2009 RF\/2009 1471624"]}],"id":[{"id":"10.13039\/spdf61vocgkt","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CJASN"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background and objectives<\/jats:title>\n                    <jats:p>\n                      Active antibody-mediated rejection is the main cause of kidney transplant loss, sharing with SLE the alloimmune response and the systemic activation of the IFN-\n                      <jats:italic toggle=\"yes\">\u03b1<\/jats:italic>\n                      pathway. IgE-mediated immune response plays a key role in the development of SLE nephritis and is associated with IFN-\n                      <jats:italic toggle=\"yes\">\u03b1<\/jats:italic>\n                      secretion. The aim of our study was to investigate IgE-mediated immune response in antibody-mediated rejection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design, setting, participants, &amp; measurements<\/jats:title>\n                    <jats:p>\n                      This was a cross-sectional study of 56 biopsy-proven antibody-mediated rejection study participants, 80 recipients with normal graft function\/histology (control), 16 study participants with interstitial fibrosis\/tubular atrophy, and six participants with SLE. We evaluated graft IgE deposition, tryptase (a mast cell marker), and CD203 (a specific marker of activated basophils) by immunofluorescence\/confocal microscopy. In addition, we measured serum concentration of human myxovirus resistance protein 1, an IFN-\n                      <jats:italic toggle=\"yes\">\u03b1\u2013<\/jats:italic>\n                      induced protein, and anti-HLA IgE.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We observed a significantly higher IgE deposition in tubules and glomeruli in antibody-mediated rejection (1766\u00b179 pixels) and SLE (1495\u00b143 pixels) compared with interstitial fibrosis\/tubular atrophy (582\u00b1122 pixels) and control (253\u00b150 pixels). Patients with antibody-mediated rejection, but not control patients and patients with interstitial fibrosis\/tubular atrophy, presented circulating anti-HLA IgE antibodies, although with a low mean fluorescence intensity. In addition, immunofluorescence revealed the presence of both mast cells and activated basophils in antibody-mediated rejection but not in control and interstitial fibrosis\/tubular atrophy. The concentration of circulating basophils was significantly higher in antibody-mediated rejection compared with control and interstitial fibrosis\/tubular atrophy. MxA serum levels were significantly higher in antibody-mediated rejection compared with control and correlated with the extent of IgE deposition.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our data suggest that IgE deposition and the subsequent recruitment of basophils and mast cells within the kidney transplant might play a role in antibody-mediated rejection.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2215\/cjn.02870320","type":"journal-article","created":{"date-parts":[[2020,9,9]],"date-time":"2020-09-09T16:00:28Z","timestamp":1599667228000},"page":"1474-1483","source":"Crossref","is-referenced-by-count":21,"title":["IgE-Mediated Immune Response and Antibody-Mediated Rejection"],"prefix":"10.2215","volume":"15","clinical-trial-number":[{"clinical-trial-number":"nct01716312","registry":"10.18810\/clinical-trials-gov"}],"author":[{"given":"Federica","family":"Rascio","sequence":"first","affiliation":[{"name":"Department of Medical and Surgical Sciences, Nephrology Dialysis and Transplantation Unit, University of Foggia, Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Pontrelli","sequence":"additional","affiliation":[{"name":"Department of Emergency and Organ Transplantation, Nephrology, Dialysis and Transplantation Unit, University of Bari \u201cA. Moro,\u201d Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe Stefano","family":"Netti","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Clinical Pathology Unit, University of Foggia, Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Manno","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Nephrology Dialysis and Transplantation Unit, University of Foggia, Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Infante","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Nephrology Dialysis and Transplantation Unit, University of Foggia, Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Simone","sequence":"additional","affiliation":[{"name":"Department of Emergency and Organ Transplantation, Nephrology, Dialysis and Transplantation Unit, University of Bari \u201cA. Moro,\u201d Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Castellano","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Nephrology Dialysis and Transplantation Unit, University of Foggia, Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Ranieri","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Clinical Pathology Unit, University of Foggia, Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michela","family":"Seveso","sequence":"additional","affiliation":[{"name":"Department of Cardiac, Thoracic and Vascular Sciences, Transplant Immunology Unit, Padova University Hospital, Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele","family":"Cozzi","sequence":"additional","affiliation":[{"name":"Department of Cardiac, Thoracic and Vascular Sciences, Transplant Immunology Unit, Padova University Hospital, Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loreto","family":"Gesualdo","sequence":"additional","affiliation":[{"name":"Department of Emergency and Organ Transplantation, Nephrology, Dialysis and Transplantation Unit, University of Bari \u201cA. Moro,\u201d Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Stallone","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Nephrology Dialysis and Transplantation Unit, University of Foggia, Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Grandaliano","sequence":"additional","affiliation":[{"name":"Department of Translational Medicine and Surgery, Nephrology Unit, Universit\u00e0 Cattolica del Sacro Cuore, Rome, Italy"},{"name":"Department of Medical and Surgical Sciences, Nephrology Unit, Fondazione Policlinico Universitario \u201cA. 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