{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:10:43Z","timestamp":1790241043334,"version":"4.1.0"},"reference-count":0,"publisher":"Forum Multimedia Publishing LLC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["eCM"],"abstract":"<jats:p>While it is known that the degenerated intervertebral disc (IVD) is one of the primary reasons for low-back pain and subsequent need for medical care, there are currently no established effective methods for direct treatment. Nuclear factor-\u03baB (NF-\u03baB) is a transcription factor that regulates various genes\u2019 expression, among which are inflammatory cytokines, in many tissues including the IVD. NF-\u03baB decoy is an oligodeoxynucleotide containing the NF-\u03baB binding site that entraps NF-\u03baB subunits, resulting in suppression of NF-\u03baB activity. In the present preclinical study, NF-\u03baB decoy was injected into degenerated IVDs using the rabbit anular-puncture model. In terms of distribution, NF-\u03baB decoy persisted in the IVDs up to at least 4 weeks after injection. The remaining amount of NF-\u03baB decoy indicated that it fit a double-exponential-decay equation. Investigation of puncture-caused degeneration of IVDs showed that NF-\u03baB decoy injection recovered, dose-dependently, the reduced disc height that was associated with reparative cell cloning and morphological changes, as assessed through histology. Gene expression, by quantitative real-time polymerase chain reaction (qRT-PCR), showed that NF-\u03baB decoy attenuated inflammatory gene expression, such as that of interleukin-1 and tumor necrosis factor-\u03b1, in rabbit degenerated IVDs. NF-\u03baB decoy also reduced the pain response as seen using the \u201cpain sensor\u201d nude rat xenograft-radiculopathy model. This is the first report demonstrating that NF-\u03baB decoy suppresses the inflammatory response in degenerated IVDs and restores IVD disc height loss. Therefore, the intradiscal injection of NF-\u03baB decoy may have the potential as an effective therapeutic strategy for discogenic pain associated with degenerated IVDs.<\/jats:p>","DOI":"10.22203\/ecm.v042a07","type":"journal-article","created":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T06:42:14Z","timestamp":1626849734000},"page":"90-109","source":"Crossref","is-referenced-by-count":14,"title":["NF-kB decoy oligodeoxynucleotide preserves disc height in a rabbit anular-puncture model and reduces pain induction in a rat xenograft-radiculopathy model"],"prefix":"10.22203","volume":"42","author":[{"name":"Department of Orthopedic Surgery, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Kato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Akeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Miyazaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Muehleman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Miyamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YA","family":"Asanuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Asanuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Fujiwara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ME","family":"Lenz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Nakazawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Masuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"39461","published-online":{"date-parts":[[2021,7,20]]},"container-title":["European Cells and Materials"],"original-title":[],"link":[{"URL":"https:\/\/www.ecmjournal.org\/papers\/vol042\/pdf\/v042a07.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T08:48:30Z","timestamp":1790239710000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ecmjournal.org\/journal\/ECM\/path\/vol042\/10.22203\/eCM.v042a07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,20]]},"references-count":0,"URL":"https:\/\/doi.org\/10.22203\/ecm.v042a07","relation":{},"subject":[],"published":{"date-parts":[[2021,7,20]]}}}