{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"Authorea, Inc."}],"indexed":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T16:40:05Z","timestamp":1748968805628,"version":"3.41.0"},"posted":{"date-parts":[[2023,4,12]]},"group-title":"Preprints","reference-count":0,"publisher":"Wiley","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2023,4,12]]},"abstract":"<jats:p id=\"p1\">Mining and industrial activity are contributing to the increase of heavy\nmetal (HM) pollution in the environment, especially in soil. These\nmetals leach into water, spread to plants and enter the food chain.\nPhytoremediation coupled to selected rhizosphere microbiota is an\nenvironmentally friendly technology designed to promote HM\nbioremediation in soils. In this study, sunflower ( <jats:italic>Helianthus\nannuus<\/jats:italic> L.) was used together with <jats:italic>Rhizophagus irregularis<\/jats:italic>, an\narbuscular mycorrhizal fungi (AMF), and <jats:italic>Cupriavidus<\/jats:italic> sp. strain\n1C2, a plant growth promoting rhizobacteria (PGPR), as a\nphytoremediation strategy to remove Zn and Cd from an industrial soil\n(599 mg Zn kg  and 1.2 mg Cd kg\n) and produce plant biomass - an agricultural soil\nwas also used to obtain a <jats:italic>H. annuus<\/jats:italic> growth and metal accumulation\ncontrol. The <jats:italic>H. annuus<\/jats:italic> biomass in the contaminated industrial\nsoil was 17% lower, at harvest than that in an agricultural soil.\nRemovals of ca. 0.04 and 0.91% of Zn and Cd respectively were obtained\nwith the biomass produced in the industrial soil in a single crop.\nBioaccumulation, remediation and translocation factors corroborated the\nhigher Zn and Cd accumulation in the roots, compared to other plants\nparts. The survival of applied microbiota was indicated by a high root\ncolonization rate of AMF and identification of strain 1C2 in the\nrhizosphere at the end of the phytoremediation assay. Changes in the\nbacterial community occurred in the industrial soil and were possibly\nassociated to the phytoremediation effect on the rhizosphere: metals\nremoval by the plant together with the synergic relationships\nestablished between AMF, PGPR and the autochthonous microbial community\nmight have favoured specific soil bacterial genera, namely\n<jats:italic>Nitrospira<\/jats:italic>, <jats:italic>Acidobacterium<\/jats:italic> and <jats:italic>Candidatus\nKoribacter<\/jats:italic>. In this study, an optimized phytomanagement strategy\napplied to a real contaminated soil was successfully tested, and plant\nbiomass with potential for upstream energetic valorisation purposes was\nproduced.<\/jats:p>","DOI":"10.22541\/au.168130633.32498772\/v1","type":"posted-content","created":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T13:32:18Z","timestamp":1681306338000},"source":"Crossref","is-referenced-by-count":1,"title":["PHYTOMANAGEMENT OF ZN AND CD CONTAMINATED SOIL: HELIANTHUS ANNUUS BIOMASS PRODUCTION AND METAL REMEDIATION ABILITIES WITH PLANT GROWTH PROMOTING MICROBIOTA ASSISTANCE"],"prefix":"10.22541","author":[{"given":"Ana P.G.C.","family":"Marques","sequence":"first","affiliation":[{"name":"Universidade Catolica Portuguesa Centro de Biotecnologia e Quimica Fina"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3058-8285","authenticated-orcid":true,"given":"Ana","family":"Paulo","sequence":"additional","affiliation":[{"name":"Universidade Catolica Portuguesa Centro de Biotecnologia e Quimica Fina"}]},{"given":"N\u00eddia S.","family":"Caetano","sequence":"additional","affiliation":[{"name":"Universidade do Porto Laboratorio de Engenharia de Processos Ambiente Biotecnologia e Energia"}]},{"given":"Paula M.L.","family":"Castro","sequence":"additional","affiliation":[{"name":"Universidade Catolica Portuguesa Centro de Biotecnologia e Quimica Fina"}]}],"member":"311","container-title":[],"original-title":[],"deposited":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T13:32:18Z","timestamp":1681306338000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.authorea.com\/users\/605847\/articles\/635145-phytomanagement-of-zn-and-cd-contaminated-soil-helianthus-annuus-biomass-production-and-metal-remediation-abilities-with-plant-growth-promoting-microbiota-assistance?commit=a91176988f3b6b70ab781f5fac8aaecd6993081c"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,12]]},"references-count":0,"URL":"https:\/\/doi.org\/10.22541\/au.168130633.32498772\/v1","relation":{},"subject":[],"published":{"date-parts":[[2023,4,12]]},"subtype":"preprint"}}