{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T04:55:06Z","timestamp":1764996906072},"reference-count":5,"publisher":"National Library of Serbia","issue":"10","license":[{"start":{"date-parts":[[2007,1,1]],"date-time":"2007-01-01T00:00:00Z","timestamp":1167609600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["VSP","VOJNOSANIT PREGL","VOJNOSANITETSKI PREG"],"published-print":{"date-parts":[[2007]]},"abstract":"<jats:p>Background\/Aim. Diazepam is a benzodiazepine anxyolitic. Metabolism of diazepam takes place in liver which generates pharmacologically active metabolites N-desmethyldiazepam, temazepam and oxazepam. The aim of this study was to develop and validate the method of liquid chromatographymass spectrometry (LC-MS) for separation and determination of diazepam and its active metabolites in the serum of rats samples after i.p. application of diazepam in a dose of 10 mg\/kg. Methods. The serum samples taken from Wistar rats, were used in LC-MS analysis after the application of 10 mg\/kg of diazepam i.p. Results. After alkaline extraction from the serum samples with diethylether and separation on a C18 reversed-phase column by using mobile phase methanolglacial acetic acid-water (50:1:49 v\/v), diazepam and its metabolites were quantified. Determination was performed in a selective ion monitoring (SIM) mode, thereby the other exogenous and endogenous compounds did not interfere with this assay. Diazepam, N-desmethyldiazepam, oxazepam and temazepam were eluted in 14 minutes. The standard curve was linear in the range from 10-2 000 ng\/ml. The limits of detection for diazepam, N-desmethyldiazepam, oxazepam and temazepam were 4.37, 3.13, 4.38 and 7.31 ng\/ml, respectively. The limits of quantitation for diazepam, Ndesmethyldiazepam, oxazepam and temazepam were 14.58, 10.41, 14.59 and 24.36 ng\/ml, respectively. Conclusion. The described LC-MS is a simple, sensitive, specific and accurate method and could be used for routine identification and quantification of small concentrations of diazepam and its metabolites in biological fluids.<\/jats:p>","DOI":"10.2298\/vsp0710659d","type":"journal-article","created":{"date-parts":[[2010,12,10]],"date-time":"2010-12-10T11:34:04Z","timestamp":1291980844000},"page":"659-662","source":"Crossref","is-referenced-by-count":3,"title":["Determination of diazepam and its metabolites in serum by the use of liquid chromatography: Mass spectrometry method"],"prefix":"10.2298","volume":"64","author":[{"given":"Snezana","family":"Djordjevic","sequence":"first","affiliation":[{"name":"Vojnomedicinska akademija, Nacionalni centar za kontrolu trovanja, Odeljenje za toksikolo\u0161ku hemiju, Beograd"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vesna","family":"Kilibarda","sequence":"additional","affiliation":[{"name":"Vojnomedicinska akademija, Nacionalni centar za kontrolu trovanja, Odeljenje za toksikolo\u0161ku hemiju, Beograd"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1078","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006943009192"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.forsciint.2006.03.027"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2003.08.068"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.12.038"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.942"}],"container-title":["Vojnosanitetski pregled","Military Medical and Pharmaceutical Journal of Serbia"],"original-title":[],"language":"en","deposited":{"date-parts":[[2023,5,29]],"date-time":"2023-05-29T11:05:47Z","timestamp":1685358347000},"score":1,"resource":{"primary":{"URL":"https:\/\/doiserbia.nb.rs\/Article.aspx?ID=0042-84500710659D"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007]]},"references-count":5,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2007]]}},"URL":"https:\/\/doi.org\/10.2298\/vsp0710659d","relation":{},"ISSN":["0042-8450","2406-0720"],"issn-type":[{"value":"0042-8450","type":"print"},{"value":"2406-0720","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007]]}}}