{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T04:06:46Z","timestamp":1685419606763},"reference-count":5,"publisher":"National Library of Serbia","issue":"7","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-sa\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["VSP","VOJNOSANIT PREGL","VOJNOSANITETSKI PREG"],"published-print":{"date-parts":[[2011]]},"abstract":"<jats:p>Background\/Aim. During peritoneal dialysis (PD) an exchange of substances\n   between blood and dialysate takes place through specific histological\n   structures of peritoneum. Peritoneal double-layered serous membrane has, so\n   far, mostly been studied with electron microscopy on experimental animals.\n   The aim of this study was to assess integrity of peritoneal tissue in\n   end-stage renal disease (ESRD) and PD patients using standard light\n   microscopy and immunohistochemical methods. Methods. Peritoneal tissue\n   biopsies were performed on 25 persons: 8 healthy donors during nephrectomy, 9\n   ESRD patients upon insertion of PD catheter, and 8 PD patients upon removal\n   of the catheter for medical indications. The samples were fixed and prepared\n   routinely for immunocytochemical staining by standardized streptavidin biotin\n   AEC method using a LSAB2? HRP kit (Dako?, Denmark) for collagen IV and\n   analyzed by light microscopy. Results. We observed mesothelial detachment\n   from lamina propria, duplicated basement membrane and much thicker blood\n   vessel walls in ESRD and PD patients, compared to healthy subjects.\n   Differences in histological structure, emphasized with immunostaining,\n   indicated pathological alterations of peritoneal tissue in the renal\n   patients. Conclusions. Imunohistochemistry can be used in studying\n   histological alterations of peritoneal tissue in ESRD and PD patients. This\n   method may indicate possible problems in filtration and secretion processes\n   in this tissue.<\/jats:p>","DOI":"10.2298\/vsp1107556t","type":"journal-article","created":{"date-parts":[[2011,7,15]],"date-time":"2011-07-15T08:03:48Z","timestamp":1310717028000},"page":"556-560","source":"Crossref","is-referenced-by-count":0,"title":["Immunohistochemical study of pathological alterations of peritoneum in patients with terminal renal insufficiency and on peritoneal dialysis"],"prefix":"10.2298","volume":"68","author":[{"given":"Jasna","family":"Trbojevic-Stankovic","sequence":"first","affiliation":[{"name":"Clinical Center Dr \u201cDragi\u0161a Mi\u0161ovi\u0107 - Dedinje\u201d, Clinic of Urology, Department of Hemodialysis, Belgrade"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miljana","family":"Obradovic","sequence":"additional","affiliation":[{"name":"School of Medicine, Institute of Histology and Embryology, Belgrade"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vesna","family":"Cemerikic-Martinovic","sequence":"additional","affiliation":[{"name":"Histolab, Belgrade"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dusan","family":"Trpinac","sequence":"additional","affiliation":[{"name":"School of Medicine, Institute of Histology and Embryology, Belgrade"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeljko","family":"Lausevic","sequence":"additional","affiliation":[{"name":"Clinical Center of Serbia, \u00a7Institute for Digestive Diseases, Belgrade"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Biljana","family":"Stojimirovic","sequence":"additional","affiliation":[{"name":"Institute of Urology, Clinic of Nephrology, Belgrade"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1078","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.2298\/VSP0201017T"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/16.3.666"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfg446"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/S1071-5576(01)00122-8"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1053\/jarr.2000.16268"}],"container-title":["Vojnosanitetski pregled","Military Medical and Pharmaceutical Journal of Serbia"],"original-title":[],"language":"en","deposited":{"date-parts":[[2023,5,29]],"date-time":"2023-05-29T11:09:59Z","timestamp":1685358599000},"score":1,"resource":{"primary":{"URL":"https:\/\/doiserbia.nb.rs\/Article.aspx?ID=0042-84501107556T"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011]]},"references-count":5,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2011]]}},"URL":"https:\/\/doi.org\/10.2298\/vsp1107556t","relation":{},"ISSN":["0042-8450","2406-0720"],"issn-type":[{"value":"0042-8450","type":"print"},{"value":"2406-0720","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011]]}}}