{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T14:50:53Z","timestamp":1767970253916,"version":"3.49.0"},"reference-count":61,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[1999,5,1]],"date-time":"1999-05-01T00:00:00Z","timestamp":925516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011084","name":"Sigma Xi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011084","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American J of Botany"],"published-print":{"date-parts":[[1999,5]]},"abstract":"<jats:p>The nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) region has been characterized in the sister genera <jats:italic>Larix<\/jats:italic> and <jats:italic>Pseudotsuga<\/jats:italic> (Pinaceae). Complete sequences were obtained for seven species of <jats:italic>Larix<\/jats:italic> from North America and Eurasia and five species of <jats:italic>Pseudotsuga<\/jats:italic> from western North America and eastern Asia. ITS region lengths ranged from 1759 to 1770 bp in <jats:italic>Larix<\/jats:italic> and from 1564 to 1571 bp in <jats:italic>Pseudotsuga.<\/jats:italic> In both genera, ITS1 is three times as long as the 5.8S plus ITS2 and contains subrepeats as observed in other genera of Pinaceae. Secondary structure models predicted that the subrepeats fold into terminal stem and loop domains. ITS polymorphism detected within individuals of <jats:italic>Larix<\/jats:italic> and <jats:italic>Pseudotsuga<\/jats:italic> suggests a slow rate of concerted evolution among nrDNA loci. Except for the placement of <jats:italic>L. sibirica,<\/jats:italic> phylogenetic analyses of the ITS region agreed with previously reported restriction site analyses of <jats:italic>Larix<\/jats:italic> and <jats:italic>Pseudotsuga.<\/jats:italic> The data were not consistent with phylogenetic hypotheses for <jats:italic>Larix<\/jats:italic> based primarily upon ovulate cone characters, failing to support a derivation of the North American <jats:italic>L. laricina<\/jats:italic> from a short\u2010bracted Eurasian lineage. The phylogenetic hypothesis did not conflict with a stepping stone model of evolution for <jats:italic>Pseudotsuga,<\/jats:italic> but a basal lineage could not be inferred for either genus.<\/jats:p>","DOI":"10.2307\/2656581","type":"journal-article","created":{"date-parts":[[2006,4,21]],"date-time":"2006-04-21T06:44:28Z","timestamp":1145601868000},"page":"711-723","source":"Crossref","is-referenced-by-count":74,"title":["Internal transcribed spacer region evolution in <i>Larix<\/i> and <i>Pseudotsuga<\/i> (Pinaceae)"],"prefix":"10.1002","volume":"86","author":[{"given":"David S.","family":"Gernandt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron","family":"Liston","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1999,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI\u2010BLAST: a new generation of protein database search programs","volume":"25","author":"Madden T. 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