{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:43:16Z","timestamp":1777513396697,"version":"3.51.4"},"reference-count":11,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2009,5,1]],"date-time":"2009-05-01T00:00:00Z","timestamp":1241136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Med Surg"],"published-print":{"date-parts":[[2009,5]]},"abstract":"\n Background:<\/jats:title>\n \n It has been demonstrated that hypertrophic scar fibroblasts (HSFs) overexpress collagen messenger ribonucleic acid (mRNA) and protein, especially \u03b1\n 1<\/jats:sub>\n collagen. Antisense nucleic acids are effective in inhibiting harmful or uncontrolled gene expression, suggesting that antisense ribonucleic acid (RNA) can effectively downregulate the expression of \u03b1\n 1<\/jats:sub>\n collagen gene and attenuate the scars.\n <\/jats:p>\n <\/jats:sec>\n \n Aims:<\/jats:title>\n \n This study was conducted to observe the effect of recombinant plasmid pREP9-COL1 on \u03b1\n 1<\/jats:sub>\n collagen expression in HSFs and clarify the prospect of antisense RNA on scar treatment.\n <\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n The \u03b1\n 1<\/jats:sub>\n collagen gene fragment including the region of 5\u2032 UTR to exon (229 bp) was cloned in the eukaryotic expression plasmid pREP9 in the antisense orientation relative to the RSV-LTR promoter to reconstruct the pREP9- COL1 plasmid. Then it was transferred into HSFs through lipofectamine. The expression of \u03b1\n 1<\/jats:sub>\n collagen was examined by immunostaining, reverse-transcriptase polymerase chain reaction, and Western blots.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n The recombinant plasmid pREP9-COL1 with a correct sequence was constructed successfully; pREP9-COL1 consistently inhibited human \u03b1\n 1<\/jats:sub>\n collagen gene expression at both mRNA and protein levels.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n Antisense RNA was effective in downregulating \u03b1\n 1<\/jats:sub>\n collagen expression of HSFs. Therefore, this approach offered a prospect of scar treatment by attenuation of \u03b1\n 1<\/jats:sub>\n collagen production with antisense RNA.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.2310\/7750.2008.07094","type":"journal-article","created":{"date-parts":[[2015,3,11]],"date-time":"2015-03-11T15:01:24Z","timestamp":1426086084000},"page":"129-133","source":"Crossref","is-referenced-by-count":0,"title":["Attenuation of \u03b1\n 1<\/sub>\n Collagen Production with Antisense Ribonucleic Acid in Cultured Hypertrophic Scar Fibroblasts"],"prefix":"10.1177","volume":"13","author":[{"given":"Xie","family":"Ju-lin","sequence":"first","affiliation":[{"name":"From the Department of Burns, The First Affiliated Hospital, and the Department of Biochemistry, Sun Yat-sen University, Guangzhou, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Shao-hai","sequence":"additional","affiliation":[{"name":"From the Department of Burns, The First Affiliated Hospital, and the Department of Biochemistry, Sun Yat-sen University, Guangzhou, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Tian-zeng","sequence":"additional","affiliation":[{"name":"From the Department of Burns, The First Affiliated Hospital, and the Department of Biochemistry, Sun Yat-sen University, Guangzhou, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luo","family":"Chao-quan","sequence":"additional","affiliation":[{"name":"From the Department of Burns, The First Affiliated Hospital, and the Department of Biochemistry, Sun Yat-sen University, Guangzhou, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Hou-dong","sequence":"additional","affiliation":[{"name":"From the Department of Burns, The First Affiliated Hospital, and the Department of Biochemistry, Sun Yat-sen University, Guangzhou, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu","family":"Ying-bin","sequence":"additional","affiliation":[{"name":"From the Department of Burns, The First Affiliated Hospital, and the Department of Biochemistry, Sun Yat-sen University, Guangzhou, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liu","family":"Xu-sheng","sequence":"additional","affiliation":[{"name":"From the Department of Burns, The First Affiliated Hospital, and the Department of Biochemistry, Sun Yat-sen University, Guangzhou, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu","family":"Bin","sequence":"additional","affiliation":[{"name":"From the Department of Burns, The First Affiliated Hospital, and the Department of Biochemistry, Sun Yat-sen University, Guangzhou, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Huizhen","sequence":"additional","affiliation":[{"name":"From the Department of Burns, The First Affiliated Hospital, and the Department of Biochemistry, Sun Yat-sen University, Guangzhou, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huang","family":"Yong","sequence":"additional","affiliation":[{"name":"From the Department of Burns, The First Affiliated Hospital, and the Department of Biochemistry, Sun Yat-sen University, Guangzhou, PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2009,5,1]]},"reference":[{"key":"bibr1-7750.2008.07094","doi-asserted-by":"publisher","DOI":"10.1016\/S1085-5629(99)80040-6"},{"key":"bibr2-7750.2008.07094","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.17.5935"},{"key":"bibr3-7750.2008.07094","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1994.tb03377.x"},{"key":"bibr4-7750.2008.07094","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)05629-X"},{"key":"bibr5-7750.2008.07094","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1993.164"},{"key":"bibr6-7750.2008.07094","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2541"},{"key":"bibr7-7750.2008.07094","doi-asserted-by":"publisher","DOI":"10.1021\/bi00202a024"},{"key":"bibr8-7750.2008.07094","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.7.3400"},{"key":"bibr9-7750.2008.07094","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2230.2007.02573.x"},{"key":"bibr10-7750.2008.07094","doi-asserted-by":"publisher","DOI":"10.1007\/s002660010038"},{"key":"bibr11-7750.2008.07094","doi-asserted-by":"publisher","DOI":"10.1021\/bi00052a002"}],"container-title":["Journal 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