{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T12:19:12Z","timestamp":1777724352297,"version":"3.51.4"},"reference-count":55,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Investigative Medicine"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      MicroRNAs (miRNAs) participate in the regulation of cardiac hypertrophy. However, it remains largely unknown as to how miRNAs are integrated into the hypertrophic program. Ca\n                      <jats:sup>2+<\/jats:sup>\n                      \/calmodulin-dependent protein kinase II (CaMKII) is a hypertrophic signaling marker. It is not yet clear which miRNAs can regulate CaMKII\u03b4.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>In this study, we identified which miRNAs could regulate CaMKII\u03b4 and how to regulate CaMKII\u03b4.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Through computational and expression analyses, miR-30b-5p was identified as a candidate regulator of CaMKII\u03b4. Quantitative expression analysis of hypertrophic models demonstrated significant down-regulation of miR-30b-5p compared with control groups. Luciferase reporter assay showed that miR-30b-5p could significantly inhibit the expression of CaMKII\u03b4. Moreover, through gain-of-function and loss-of-function approaches, we found miR-30b-5p could negatively regulate the expression of CaMKII\u03b4 and miR-30b-5p was a regulator of cardiac hypertrophy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study demonstrates that the expression of miR-30b-5p is down-regulated in cardiac hypertrophy, and restoration of its function inhibits the expression of CaMKII\u03b4, suggesting that miR-30b-5p may act as a hypertrophic suppressor.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2310\/jim.0b013e3182819ac6","type":"journal-article","created":{"date-parts":[[2016,5,11]],"date-time":"2016-05-11T09:10:57Z","timestamp":1462957857000},"page":"604-612","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":28,"title":["MicroRNA-30b-5p is Involved in the Regulation of Cardiac Hypertrophy by Targeting CaMKII\u03b4"],"prefix":"10.1177","volume":"61","author":[{"given":"Juan","family":"He","sequence":"first","affiliation":[{"name":"From the Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"Jiang","sequence":"additional","affiliation":[{"name":"From the Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng-lan","family":"Li","sequence":"additional","affiliation":[{"name":"From the Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue-jiao","family":"Zhao","sequence":"additional","affiliation":[{"name":"From the Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Er-fu","family":"Chu","sequence":"additional","affiliation":[{"name":"From the Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei-na","family":"Sun","sequence":"additional","affiliation":[{"name":"From the Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-zi","family":"Chen","sequence":"additional","affiliation":[{"name":"From the Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"From the Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, China."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,3,1]]},"reference":[{"key":"bibr1-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1038\/ajh.2008.16"},{"key":"bibr2-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.65.092101.142243"},{"key":"bibr3-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1983"},{"key":"bibr4-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1172\/JCI38022"},{"key":"bibr5-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1005843107"},{"key":"bibr6-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.57.030195.002221"},{"key":"bibr7-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1210\/mend.14.1.0414"},{"key":"bibr8-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2003.08.023"},{"key":"bibr9-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.84.6.713"},{"key":"bibr10-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1210\/me.2002-0350"},{"key":"bibr11-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813013106"},{"key":"bibr12-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108525200"},{"key":"bibr13-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000069686.31472.C5"},{"key":"bibr14-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1038\/nm1215"},{"key":"bibr15-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400783"},{"key":"bibr16-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)01018-3"},{"key":"bibr17-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.002"},{"key":"bibr18-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.61"},{"key":"bibr19-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.20405"},{"key":"bibr20-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2146903"},{"key":"bibr21-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-200204000-00036"},{"key":"bibr22-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp272"},{"key":"bibr23-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1038\/ng1798"},{"key":"bibr24-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M305371200"},{"key":"bibr25-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.11.2757"},{"key":"bibr26-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90529-Y"},{"key":"bibr27-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608791103"},{"key":"bibr28-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000257913.42552.23"},{"key":"bibr29-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2006.11.036"},{"key":"bibr30-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0712265105"},{"key":"bibr31-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81573-1"},{"key":"bibr32-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.19.8374-8385.2004"},{"key":"bibr33-JIM.0b013e3182819ac6","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1111\/j.1440-1681.2010.05382.x","volume":"37","author":"Zhang W","year":"2010","journal-title":"Clin Exp Pharmacol Physiol"},{"key":"bibr34-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2005000900002"},{"key":"bibr35-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045727"},{"key":"bibr36-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.292722"},{"key":"bibr37-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-7-r64"},{"key":"bibr38-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000795"},{"key":"bibr39-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.169"},{"key":"bibr40-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.12.121"},{"key":"bibr41-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1017346108"},{"key":"bibr42-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.167"},{"key":"bibr43-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.182535"},{"key":"bibr44-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.027896"},{"key":"bibr45-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1038\/nm1582"},{"key":"bibr46-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1126\/science.1139089"},{"key":"bibr47-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1038\/ng1803"},{"key":"bibr48-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1038\/ng1953"},{"key":"bibr49-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1126\/science.1109020"},{"key":"bibr50-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1500707"},{"key":"bibr51-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1038\/nature07242"},{"key":"bibr52-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1038\/nature07228"},{"key":"bibr53-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1210\/rp.59.1.141"},{"key":"bibr54-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01222-08"},{"key":"bibr55-JIM.0b013e3182819ac6","doi-asserted-by":"publisher","DOI":"10.1038\/nature08195"}],"container-title":["Journal of Investigative Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.2310\/JIM.0b013e3182819ac6","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.2310\/JIM.0b013e3182819ac6","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.2310\/JIM.0b013e3182819ac6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T10:39:43Z","timestamp":1777459183000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.2310\/JIM.0b013e3182819ac6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3]]},"references-count":55,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["10.2310\/JIM.0b013e3182819ac6"],"URL":"https:\/\/doi.org\/10.2310\/jim.0b013e3182819ac6","relation":{},"ISSN":["1081-5589","1708-8267"],"issn-type":[{"value":"1081-5589","type":"print"},{"value":"1708-8267","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3]]}}}