{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T15:08:42Z","timestamp":1778684922269,"version":"3.51.4"},"reference-count":34,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Journal of Investigative Medicine"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Nonsyndromic cleft lip and cleft palate (CL\/P) is associated with environmental, nutritional, and genetic factors. Maternal polymorphisms in the methylenetetrahydrofolate reductase ( MTHFR) gene have been associated with CL\/P.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To determine the relationship between the risk of having a child with CL\/P and maternal C677T and A1298C MTHFR polymorphisms, the intake of folate supplements, and exposure to environmental factors during the first trimester of pregnancy, a case-control study of Mexican mothers (88 case mothers and 116 control mothers) was conducted.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A questionnaire was used to assess exposure to environmental factors. The C677T and A1298C polymorphisms were identified by polymerase chain reaction with restriction fragment length polymorphism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Mothers with the 677CT or 677TT genotype had a higher risk of having a child with CL\/P than mothers with the 677CC genotype (odds ratio [OR], 2.4; 95% confidence interval [CI], 1.1\u20135.7). An increased risk of having a child with CL\/P was associated with the lack of folate supplementation during the first trimester of pregnancy (OR, 3.8; 95% CI, 1.9\u20137.6), and this risk was greater in the mothers with the 677TT or 677CT genotype than mothers who reported taking folate supplements and had the 677CC genotype (OR, 11.2; 95% CI, 3.3\u201337.5). Pesticide exposure was associated with CL\/P. There was no significant association between either the A1298C variant or tobacco exposure and the risk of CL\/P.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These results suggest that gene-environment interactions play an important role in the development of CL\/P.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2310\/jim.0b013e31829a7e7e","type":"journal-article","created":{"date-parts":[[2016,5,11]],"date-time":"2016-05-11T09:10:57Z","timestamp":1462957857000},"page":"1030-1035","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Maternal C677T MTHFR Polymorphism and Environmental Factors are Associated with Cleft Lip and Palate in a Mexican Population"],"prefix":"10.1177","volume":"61","author":[{"given":"Jose Jesus","family":"Ibarra-Lopez","sequence":"first","affiliation":[{"name":"Laboratory of Toxicology and Environmental Health, Academic Unit of Chemical-Biological Sciences, Autonomous University of Guerrero, Chilpancingo, Gro, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Duarte","sequence":"additional","affiliation":[{"name":"Department of Maxillofacial Surgery, General Hospital Dr. Raymundo Abarca Alarcon, Chilpancingo, Gro, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronica","family":"Antonio-Vejar","sequence":"additional","affiliation":[{"name":"Laboratory of Toxicology and Environmental Health, Academic Unit of Chemical-Biological Sciences, Autonomous University of Guerrero, Chilpancingo, Gro, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma S.","family":"Calderon-Aranda","sequence":"additional","affiliation":[{"name":"Department of Toxicology, CINVESTAV, Mexico, DF, Mexico."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerardo","family":"Huerta-Beristain","sequence":"additional","affiliation":[{"name":"Laboratory of Toxicology and Environmental Health, Academic Unit of Chemical-Biological Sciences, Autonomous University of Guerrero, Chilpancingo, Gro, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugenia","family":"Flores-Alfaro","sequence":"additional","affiliation":[{"name":"Laboratory of Toxicology and Environmental Health, Academic Unit of Chemical-Biological Sciences, Autonomous University of Guerrero, Chilpancingo, Gro, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma Elena","family":"Moreno-Godinez","sequence":"additional","affiliation":[{"name":"Laboratory of Toxicology and Environmental Health, Academic Unit of Chemical-Biological Sciences, Autonomous University of Guerrero, Chilpancingo, Gro, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,8,1]]},"reference":[{"key":"bibr1-JIM.0b013e31829a7e7e","doi-asserted-by":"publisher","DOI":"10.1053\/j.sodo.2008.02.002"},{"key":"bibr2-JIM.0b013e31829a7e7e","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/26.1.7"},{"key":"bibr3-JIM.0b013e31829a7e7e","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2933"},{"key":"bibr4-JIM.0b013e31829a7e7e","doi-asserted-by":"publisher","DOI":"10.1016\/j.anpedi.2011.01.011"},{"key":"bibr5-JIM.0b013e31829a7e7e","unstructured":"SSA. 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