{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T03:49:02Z","timestamp":1790221742704,"version":"4.1.0"},"reference-count":34,"publisher":"The Angle Orthodontist (EH Angle Education & Research Foundation)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n        <jats:p>\n          <jats:bold>Objective:<\/jats:bold> To develop a new cementoblast culture method and to detect bone sialoprotein (BSP) expression in response to high and low mechanical tensile stress in cementoblast in vitro.<\/jats:p>\n        <jats:p>\n          <jats:bold>Materials and Methods:<\/jats:bold> Cementoblasts were collected from the roots of newborn bovine teeth and were identified with cementum-derived attachment protein (CAP) antibody 3G9. Cell proliferation was evaluated by MTT [3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay, and mineralization was confirmed by von Kossa staining. Mechanical tensile stress was applied in vitro to the cementoblast with the use of a uniaxial four-point bending system with 2000 or 4000 microstrains, at a frequency of 0.5 Hz for 3, 6, 12, 24, or 36 hours. BSP mRNA level was quantified by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR).<\/jats:p>\n        <jats:p>\n          <jats:bold>Results:<\/jats:bold> A large amount of cementoblast was observed to be expressing CAP. Cementoblasts had a proliferation tendency similar to that of osteoblasts but different from that of periodontal ligament (PDL) cells. Cementoblasts had the ability to become mineralized between osteoblasts and PDL cells. The mechanical tensile stress significantly up-regulated BSP mRNA expression, which reached a peak at 24 hours in both 2000 and 4000 microstrain groups (<jats:italic>P<\/jats:italic> &lt; .01) and was tenfold and sixfold higher than that of controls, respectively. BSP expression dropped toward baseline levels at 36 hours in both groups.<\/jats:p>\n        <jats:p>\n          <jats:bold>Conclusions:<\/jats:bold> Mechanical tensile stress up-regulated the expression of BSP. Low mechanical tensile stress induced earlier and more intensive up-regulation of BSP mRNA; this might represent the optimal stimuli for cementoblast activity.<\/jats:p>","DOI":"10.2319\/011508-20.1","type":"journal-article","created":{"date-parts":[[2008,9,24]],"date-time":"2008-09-24T19:34:28Z","timestamp":1222284868000},"page":"346-352","source":"Crossref","is-referenced-by-count":16,"title":["Mechanical Tensile Stress Effects on the Expression of Bone Sialoprotein in Bovine Cementoblasts"],"prefix":"10.2319","volume":"79","author":[{"given":"Hongyou","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijin","family":"Ren","sequence":"additional","affiliation":[{"name":"b\u2009Professor, Department of Orthodontics, University Medical Center Groningen (UMCG), University of Groningen, Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Sandham","sequence":"additional","affiliation":[{"name":"b\u2009Professor, Department of Orthodontics, University Medical Center Groningen (UMCG), University of Groningen, Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aishu","family":"Ren","sequence":"additional","affiliation":[{"name":"c\u2009Resident, Department of Orthodontics, Chongqing Stomatology Hospital, Chongqing Medical University, Chongqing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Huang","sequence":"additional","affiliation":[{"name":"d\u2009Graduate Student (PhD), Department of Orthodontics, West China Stomatology Hospital, Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ding","family":"Bai","sequence":"additional","affiliation":[{"name":"e\u2009Professor, State Key Laboratory of Oral Disease, Department of Orthodontics, West China Stomatology Hospital, Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1164","reference":[{"key":"i0003-3219-079-02-0346-b1","series-title":"Orthod Craniofac Res","first-page":"64","article-title":"Exploring the third dimension in root resorption.","volume":"7","author":"Chan, E.","year":"2004","unstructured":"Chan, E. and M. A.Darendeliler. Exploring the third dimension in root resorption.Orthod Craniofac Res2004. 7:64\u201370."},{"key":"i0003-3219-079-02-0346-b2","series-title":"Am J Orthod Dentofacial Orthop","first-page":"504","article-title":"Physical properties of root cementum: part 7. Extent of root resorption under areas of compression and tension.","volume":"129","author":"Chan, E.","year":"2006","unstructured":"Chan, E. and M. A.Darendeliler. Physical properties of root cementum: part 7. Extent of root resorption under areas of compression and tension.Am J Orthod Dentofacial Orthop2006. 129:504\u2013510."},{"key":"i0003-3219-079-02-0346-b3","series-title":"Eur J Orthod","first-page":"189","article-title":"The repair of orthodontic root resorption: an ultrastructural study.","volume":"17","author":"Brudevik, P.","year":"1995","unstructured":"Brudevik, P. and P.Rygh. The repair of orthodontic root resorption: an ultrastructural study.Eur J Orthod1995. 17:189\u2013198."},{"key":"i0003-3219-079-02-0346-b4","series-title":"Periodontol 2000","first-page":"73","article-title":"Molecular and cell biology of cementum.","volume":"24","author":"Saygin, N. E.","year":"2000","unstructured":"Saygin, N. E., W. V.Giannobile, and M. J.Somerman. Molecular and cell biology of cementum.Periodontol 20002000. 24:73\u201398."},{"key":"i0003-3219-079-02-0346-b5","series-title":"Periodontol 2000","first-page":"41","article-title":"Dental cementum: the dynamic tissue covering of the root.","volume":"13","author":"Bosshardt, D. D.","year":"1997","unstructured":"Bosshardt, D. D. and K. A.Selvig. Dental cementum: the dynamic tissue covering of the root.Periodontol 20001997. 13:41\u201375."},{"key":"i0003-3219-079-02-0346-b6","series-title":"J Dent Res","first-page":"390","article-title":"Are cementoblasts a subpopulation of osteoblasts or a unique phenotype?","volume":"84","author":"Bosshardt, D. D.","year":"2005","unstructured":"Bosshardt, D. D.\n          \n          Are cementoblasts a subpopulation of osteoblasts or a unique phenotype?\n          J Dent Res\n          2005. 84:390\u2013406."},{"key":"i0003-3219-079-02-0346-b7","series-title":"J Struct Biol","first-page":"256","article-title":"Content and distribution of noncollagenous matrix proteins in bone and cementum: relationship to speed of formation and collagen packing density.","volume":"126","author":"Nanci, A.","year":"1999","unstructured":"Nanci, A.\n          \n          Content and distribution of noncollagenous matrix proteins in bone and cementum: relationship to speed of formation and collagen packing density.\n          J Struct Biol\n          1999. 126:256\u2013269."},{"key":"i0003-3219-079-02-0346-b8","series-title":"J Periodontol","first-page":"1665","article-title":"Immunohistochemical study of bone sialoprotein and osteopontin in healthy and diseased root surfaces.","volume":"77","author":"Lao, M.","year":"2006","unstructured":"Lao, M., V.Marino, and P. M.Bartold. Immunohistochemical study of bone sialoprotein and osteopontin in healthy and diseased root surfaces.J Periodontol2006. 77:1665\u20131673."},{"key":"i0003-3219-079-02-0346-b9","series-title":"Crit Rev Oral Biol Med","first-page":"414","article-title":"Effect of mechanical loading on periodontal cells.","volume":"12","author":"Pavlin, D.","year":"2001","unstructured":"Pavlin, D. and J.Gluhak-Heinrich. Effect of mechanical loading on periodontal cells.Crit Rev Oral Biol Med2001. 12:414\u2013424."},{"key":"i0003-3219-079-02-0346-b10","series-title":"J Dent Res","first-page":"1272","article-title":"Cell attachment activity of cementum proteins and mechanism of endotoxin inhibition.","volume":"70","author":"Olson, S.","year":"1991","unstructured":"Olson, S., H.Arzate, A. S.Narayanan, and R. C.Page. Cell attachment activity of cementum proteins and mechanism of endotoxin inhibition.J Dent Res1991. 70:1272\u20131277."},{"key":"i0003-3219-079-02-0346-b11","series-title":"J Bone Miner Res","first-page":"686","article-title":"Characterization of a collagenous cementum-derived attachment protein.","volume":"11","author":"Wu, D.","year":"1996","unstructured":"Wu, D., K.Ikezawa, T.Parker, M.Saito, and A. S.Narayanan. Characterization of a collagenous cementum-derived attachment protein.J Bone Miner Res1996. 11:686\u2013692."},{"key":"i0003-3219-079-02-0346-b12","series-title":"J Periodontal Res","first-page":"249","article-title":"Human cementum tumor cells have different features from human osteoblastic cells in vitro.","volume":"33","author":"Arzate, H.","year":"1998","unstructured":"Arzate, H., M. A.Alvarez-Perez, M. E.Aguilar-Mendoza, and O.Alvarez-Fregoso. Human cementum tumor cells have different features from human osteoblastic cells in vitro.J Periodontal Res1998. 33:249\u2013258."},{"key":"i0003-3219-079-02-0346-b13","series-title":"Connect Tissue Res","first-page":"309","article-title":"Characterization of cementum derived growth factor as an insulin-like growth factor-I like molecule.","volume":"36","author":"Ikezawa, K.","year":"1997","unstructured":"Ikezawa, K., C. E.Hart, D. C.Williams, and A. S.Narayanan. Characterization of cementum derived growth factor as an insulin-like growth factor-I like molecule.Connect Tissue Res1997. 36:309\u2013319."},{"key":"i0003-3219-079-02-0346-b14","series-title":"Bone Miner","first-page":"187","article-title":"Isolation and partial characterization of a growth factor from human cementum.","volume":"18","author":"Yonemura, K.","year":"1992","unstructured":"Yonemura, K., A. S.Narayanan, Y.Miki, R. C.Page, and H.Okada. Isolation and partial characterization of a growth factor from human cementum.Bone Miner1992. 18:187\u2013198."},{"key":"i0003-3219-079-02-0346-b15","series-title":"Bone","first-page":"606","article-title":"Cementum matrix formation in vivo by cultured dental follicle cells.","volume":"31","author":"Handa, K.","year":"2002","unstructured":"Handa, K., M.Saito, and M.Yamauchi. et al. Cementum matrix formation in vivo by cultured dental follicle cells.Bone2002. 31:606\u2013611."},{"key":"i0003-3219-079-02-0346-b16","series-title":"Bone","first-page":"242","article-title":"Expression of cementum-derived attachment protein in bovine tooth germ during cementogenesis.","volume":"29","author":"Saito, M.","year":"2001","unstructured":"Saito, M., M.Iwase, and S.Maslan. et al. Expression of cementum-derived attachment protein in bovine tooth germ during cementogenesis.Bone2001. 29:242\u2013248."},{"key":"i0003-3219-079-02-0346-b17","series-title":"Connect Tissue Res","first-page":"19","article-title":"Cementum specific components which influence periodontal connective tissue cells.","volume":"33","author":"Narayanan, A. S.","year":"1995","unstructured":"Narayanan, A. S., K.Ikezawa, D.Wu, and S.Pitaru. Cementum specific components which influence periodontal connective tissue cells.Connect Tissue Res1995. 33:19\u201321."},{"key":"i0003-3219-079-02-0346-b18","series-title":"Eur J Oral Sci","first-page":"239","article-title":"Amelogenins regulate expression of genes associated with cementoblasts in vitro.","volume":"114","author":"Swanson, E. C.","year":"2006","unstructured":"Swanson, E. C., H. K.Fong, and B. L.Foster. et al. Amelogenins regulate expression of genes associated with cementoblasts in vitro.Eur J Oral Sci2006. 114:(suppl 1). 239\u2013243. discussion 254\u2013256, 381\u2013382."},{"key":"i0003-3219-079-02-0346-b19","series-title":"J Bone Miner Res","first-page":"246","article-title":"JunB as a downstream mediator of PTHrP actions in cementoblasts.","volume":"21","author":"Berry, J. E.","year":"2006","unstructured":"Berry, J. E., E. L.Ealba, and G. J.Pettway. et al. JunB as a downstream mediator of PTHrP actions in cementoblasts.J Bone Miner Res2006. 21:246\u2013257."},{"key":"i0003-3219-079-02-0346-b20","series-title":"J Cell Biochem","first-page":"497","article-title":"Predominant integrin ligands expressed by osteoblasts show preferential regulation in response to both cell adhesion and mechanical perturbation.","volume":"84","author":"Carvalho, R. S.","year":"2002","unstructured":"Carvalho, R. S., A.Bumann, J. L.Schaffer, and L. C.Gerstenfeld. Predominant integrin ligands expressed by osteoblasts show preferential regulation in response to both cell adhesion and mechanical perturbation.J Cell Biochem2002. 84:497\u2013508."},{"key":"i0003-3219-079-02-0346-b21","series-title":"Calcif Tissue Int","first-page":"103","article-title":"Regulation of cementoblast gene expression by inorganic phosphate in vitro.","volume":"78","author":"Foster, B. L.","year":"2006","unstructured":"Foster, B. L., F. H.NocitiJr, and E. C.Swanson. et al. Regulation of cementoblast gene expression by inorganic phosphate in vitro.Calcif Tissue Int2006. 78:103\u2013112."},{"key":"i0003-3219-079-02-0346-b22","series-title":"Life Sci","first-page":"3168","article-title":"Optimal compressive force induces bone formation via increasing bone sialoprotein and prostaglandin E(2) production appropriately.","volume":"77","author":"Mitsui, N.","year":"2005","unstructured":"Mitsui, N., N.Suzuki, and M.Maeno. et al. Optimal compressive force induces bone formation via increasing bone sialoprotein and prostaglandin E(2) production appropriately.Life Sci2005. 77:3168\u20133182."},{"key":"i0003-3219-079-02-0346-b23","series-title":"Bone","first-page":"117","article-title":"Expression of bone associated markers by tooth root lining cells, in situ and in vitro.","volume":"20","author":"D'Errico, J. A.","year":"1997","unstructured":"D'Errico, J. A., R. L.MacNeil, T.Takata, J.Berry, C.Strayhorn, and M. J.Somerman. Expression of bone associated markers by tooth root lining cells, in situ and in vitro.Bone1997. 20:117\u2013126."},{"key":"i0003-3219-079-02-0346-b24","series-title":"J Periodontol","first-page":"63","article-title":"Employing a transgenic animal model to obtain cementoblasts in vitro.","volume":"71","author":"D'Errico, J. A.","year":"2000","unstructured":"D'Errico, J. A., J. E.Berry, H.Ouyang, C. L.Strayhorn, J. J.Windle, and M. J.Somerman. Employing a transgenic animal model to obtain cementoblasts in vitro.J Periodontol2000. 71:63\u201372."},{"key":"i0003-3219-079-02-0346-b25","series-title":"Bone","first-page":"220","article-title":"Establishment of cementoblast cell lines from rat cementum lining cells by transfection with temperature-sensitive simian virus-40 T-antigen gene.","volume":"37","author":"Kitagawa, M.","year":"2005","unstructured":"Kitagawa, M., S.Kitagawa, and Y.Kudo. et al. Establishment of cementoblast cell lines from rat cementum lining cells by transfection with temperature-sensitive simian virus-40 T-antigen gene.Bone2005. 37:220\u2013226."},{"key":"i0003-3219-079-02-0346-b26","series-title":"J Bone Miner Res","first-page":"1547","article-title":"Normal human cementum-derived cells: isolation, clonal expansion, and in vitro and in vivo characterization.","volume":"13","author":"Grzesik, W. J.","year":"1998","unstructured":"Grzesik, W. J., S. A.Kuzentsov, K.Uzawa, M.Mankani, P. G.Robey, and M.Yamauchi. Normal human cementum-derived cells: isolation, clonal expansion, and in vitro and in vivo characterization.J Bone Miner Res1998. 13:1547\u20131554."},{"key":"i0003-3219-079-02-0346-b27","series-title":"Arthritis Rheum","first-page":"1500","article-title":"Actions of recombinant human gamma-interferon and tumor necrosis factor alpha on the proliferation and osteoblastic characteristics of human trabecular bone cells in vitro.","volume":"31","author":"Gowen, M.","year":"1988","unstructured":"Gowen, M., B. R.MacDonald, and R. G.Russell. Actions of recombinant human gamma-interferon and tumor necrosis factor alpha on the proliferation and osteoblastic characteristics of human trabecular bone cells in vitro.Arthritis Rheum1988. 31:1500\u20131507."},{"key":"i0003-3219-079-02-0346-b28","series-title":"Eur J Orthod","first-page":"339","article-title":"Temporal and spatial mRNA expression of bone sialoprotein and type I collagen during rodent tooth movement.","volume":"23","author":"Domon, S.","year":"2001","unstructured":"Domon, S., H.Shimokawa, S.Yamaguchi, and K.Soma. Temporal and spatial mRNA expression of bone sialoprotein and type I collagen during rodent tooth movement.Eur J Orthod2001. 23:339\u2013348."},{"key":"i0003-3219-079-02-0346-b29","series-title":"Curr Opin Clin Nutr Metab Care","first-page":"289","article-title":"Signal transduction by mechanical strain in chondrocytes.","volume":"6","author":"Deschner, J.","year":"2003","unstructured":"Deschner, J., C. R.Hofman, N. P.Piesco, and S.Agarwal. Signal transduction by mechanical strain in chondrocytes.Curr Opin Clin Nutr Metab Care2003. 6:289\u2013293."},{"key":"i0003-3219-079-02-0346-b30","series-title":"Exp Cell Res","first-page":"66","article-title":"Effects of intermittent or continuous gravitational stresses on cell-matrix adhesion: quantitative analysis of focal contacts in osteoblastic ROS 17\/2.8 cells.","volume":"236","author":"Guignandon, A.","year":"1997","unstructured":"Guignandon, A., Y.Usson, and N.Laroche. et al. Effects of intermittent or continuous gravitational stresses on cell-matrix adhesion: quantitative analysis of focal contacts in osteoblastic ROS 17\/2.8 cells.Exp Cell Res1997. 236:66\u201375."},{"key":"i0003-3219-079-02-0346-b31","series-title":"Bone","first-page":"609","article-title":"Involvement of different ion channels in osteoblasts' and osteocytes' early responses to mechanical strain.","volume":"19","author":"Rawlinson, S. C.","year":"1996","unstructured":"Rawlinson, S. C., A. A.Pitsillides, and L. E.Lanyon. Involvement of different ion channels in osteoblasts' and osteocytes' early responses to mechanical strain.Bone1996. 19:609\u2013614."},{"key":"i0003-3219-079-02-0346-b32","series-title":"J Biomech","first-page":"3","article-title":"Techniques for mechanical stimulation of cells in vitro: a review.","volume":"33","author":"Brown, T. D.","year":"2000","unstructured":"Brown, T. D.\n          \n          Techniques for mechanical stimulation of cells in vitro: a review.\n          J Biomech\n          2000. 33:3\u201314."},{"key":"i0003-3219-079-02-0346-b33","series-title":"In Vitro Cell Dev Biol","first-page":"500","article-title":"Mechanical effects on endothelial cell morphology: in vitro assessment.","volume":"22","author":"Ives, C. L.","year":"1986","unstructured":"Ives, C. L., S. G.Eskin, and L. V.McIntire. Mechanical effects on endothelial cell morphology: in vitro assessment.In Vitro Cell Dev Biol1986. 22:500\u2013507."},{"key":"i0003-3219-079-02-0346-b34","series-title":"Exp Cell Res","first-page":"285","article-title":"A new in vitro system for studying cell response to mechanical stimulation: different effects of cyclic stretching and agitation on smooth muscle cell biosynthesis.","volume":"109","author":"Leung, D. Y.","year":"1977","unstructured":"Leung, D. Y., S.Glagov, and M. B.Mathews. A new in vitro system for studying cell response to mechanical stimulation: different effects of cyclic stretching and agitation on smooth muscle cell biosynthesis.Exp Cell Res1977. 109:285\u2013298."}],"container-title":["The Angle Orthodontist"],"original-title":[],"link":[{"URL":"https:\/\/angle-orthodontist.kglmeridian.com\/view\/journals\/angl\/79\/2\/article-p346.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/meridian.allenpress.com\/angle-orthodontist\/article-pdf\/79\/2\/346\/1388180\/011508-20_1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/angle-orthodontist.kglmeridian.com\/downloadpdf\/journals\/angl\/79\/2\/article-p346.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/angle-orthodontist.kglmeridian.com\/downloadpdf\/journals\/angl\/79\/2\/article-p346.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T00:46:19Z","timestamp":1747356379000},"score":1,"resource":{"primary":{"URL":"https:\/\/angle-orthodontist.kglmeridian.com\/view\/journals\/angl\/79\/2\/article-p346.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3]]},"references-count":34,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.2319\/011508-20.1","relation":{},"ISSN":["0003-3219","1945-7103"],"issn-type":[{"value":"0003-3219","type":"print"},{"value":"1945-7103","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,3]]}}}