{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:15:32Z","timestamp":1792667732479,"version":"4.5.0"},"reference-count":54,"publisher":"American Diabetes Association","issue":"9","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,9,1]]},"abstract":"<jats:p>Homeostasis of blood glucose is mainly regulated by the coordinated secretion of glucagon and insulin from \u03b1- and \u03b2-cells within the islets of Langerhans. The release of both hormones is Ca2+ dependent. In the current study, we used confocal microscopy and immunocytochemistry to unequivocally characterize the glucose-induced Ca2+ signals in \u03b1- and \u03b2-cells within intact human islets. Extracellular glucose stimulation induced an opposite response in these two cell types. Although the intracellular Ca2+ concentration ([Ca2+]i) in \u03b2-cells remained stable at low glucose concentrations, \u03b1-cells exhibited an oscillatory [Ca2+]i response. Conversely, the elevation of extracellular glucose elicited an oscillatory [Ca2+]i pattern in \u03b2-cells but inhibited low-glucose\u2013induced [Ca2+]i signals in \u03b1-cells. These Ca2+ signals were synchronic among \u03b2-cells grouped in clusters within the islet, although they were not coordinated among the whole \u03b2-cell population. The response of \u03b1-cells was totally asynchronic. Therefore, both the \u03b1- and \u03b2-cell populations within human islets did not work as a syncitium in response to glucose. A deeper knowledge of \u03b1- and \u03b2-cell behavior within intact human islets is important to better understand the physiology of the human endocrine pancreas and may be useful to select high-quality islets for transplantation.<\/jats:p>","DOI":"10.2337\/db06-0272","type":"journal-article","created":{"date-parts":[[2006,8,25]],"date-time":"2006-08-25T21:48:55Z","timestamp":1156542535000},"page":"2463-2469","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":94,"title":["Glucose Induces Opposite Intracellular Ca2+ Concentration Oscillatory Patterns in Identified \u03b1- and \u03b2-Cells Within Intact Human Islets of Langerhans"],"prefix":"10.2337","volume":"55","author":[{"given":"Ivan","family":"Quesada","sequence":"first","affiliation":[{"name":"Institute of Bioengineering, Miguel Hernandez University, Sant Joan d\u2019Alacant, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana G.","family":"Todorova","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Miguel Hernandez University, Sant Joan d\u2019Alacant, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paloma","family":"Alonso-Magdalena","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Miguel Hernandez University, Sant Joan d\u2019Alacant, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Beltra\u0301","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Miguel Hernandez University, Sant Joan d\u2019Alacant, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Everardo M.","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biology, State University of Campinas, Campinas, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franz","family":"Martin","sequence":"additional","affiliation":[{"name":"Andalusian Center of Developmental Biology, University Pablo Olavide, Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"Nadal","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering, Miguel Hernandez University, Sant Joan d\u2019Alacant, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernat","family":"Soria","sequence":"additional","affiliation":[{"name":"CABIMER (Andalusian Center for Molecular Biology and Regenerative Medicine), Seville, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","reference":[{"key":"2022031208045111900_R1","doi-asserted-by":"crossref","unstructured":"Shapiro AMJ, Lakey JRT, Ryan EA, Korbutt GS, Toth EL, Warnock GL, Kneteman NN, Rajotte RV: Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. 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