{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T09:06:51Z","timestamp":1790327211164,"version":"4.1.0"},"reference-count":27,"publisher":"American Diabetes Association","issue":"11","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,11,1]]},"abstract":"<jats:p>OBJECTIVE\u2014 Recent observations indicate that the delivery of nitric oxide by endothelial nitric oxide synthase (eNOS) is not only critical for metabolic homeostasis, but could also be important for mitochondrial biogenesis, a key organelle for free fatty acid (FFA) oxidation and energy production. Because mice deficient for the gene of eNOS (eNOS\u2212\/\u2212) have increased triglycerides and FFA levels, in addition to hypertension and insulin resistance, we hypothesized that these knockout mice may have decreased energy expenditure and defective \u03b2-oxidation.<\/jats:p>\n                  <jats:p>RESEARCH DESIGN AND METHODS\u2014 Several markers of mitochondrial activity were assessed in C57BL\/6J wild-type or eNOS\u2212\/\u2212 mice including the energy expenditure and oxygen consumption by indirect calorimetry, in vitro \u03b2-oxidation in isolated mitochondria from skeletal muscle, and expression of genes involved in fatty acid oxidation.<\/jats:p>\n                  <jats:p>RESULTS\u2014 eNOS\u2212\/\u2212 mice had markedly lower energy expenditure (\u221210%, P &amp;lt; 0.05) and oxygen consumption (\u221215%, P &amp;lt; 0.05) than control mice. This was associated with a roughly 30% decrease of the mitochondria content (P &amp;lt; 0.05) and, most importantly, with mitochondrial dysfunction, as evidenced by a markedly lower \u03b2-oxidation of subsarcolemmal mitochondria in skeletal muscle (\u221230%, P &amp;lt; 0.05). Finally, impaired mitochondrial \u03b2-oxidation was associated with a significant increase of the intramyocellular lipid content (30%, P &amp;lt; 0.05) in gastrocnemius muscle.<\/jats:p>\n                  <jats:p>CONCLUSIONS\u2014 These data indicate that elevated FFA and triglyceride in eNOS\u2212\/\u2212 mice result in defective mitochondrial \u03b2-oxidation in muscle cells.<\/jats:p>","DOI":"10.2337\/db06-1228","type":"journal-article","created":{"date-parts":[[2007,9,5]],"date-time":"2007-09-05T17:00:14Z","timestamp":1189011614000},"page":"2690-2696","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":114,"title":["Endothelial Nitric Oxide Synthase (eNOS) Knockout Mice Have Defective Mitochondrial \u03b2-Oxidation"],"prefix":"10.2337","volume":"56","author":[{"given":"Eric Le","family":"Gouill","sequence":"first","affiliation":[{"name":"Department of Cellular Biology and Morphology, University of Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Jimenez","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Physiology, CMU, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Binnert","sequence":"additional","affiliation":[{"name":"Institute of Physiology, University of Lausanne, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre-Yves","family":"Jayet","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and the Botnar Center for Clinical Research, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastien","family":"Thalmann","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and the Botnar Center for Clinical Research, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Nicod","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and the Botnar Center for Clinical Research, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Urs","family":"Scherrer","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and the Botnar Center for Clinical Research, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Vollenweider","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and the Botnar Center for Clinical Research, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","reference":[{"key":"2022031208341771300_R1","doi-asserted-by":"crossref","unstructured":"Duplain H, Burcelin R, Sartori C, Cook S, Egli M, Lepori M, Vollenweider P, Pedrazzini T, Nicod P, Thorens B, Scherrer U: Insulin resistance, hyperlipidemia, and hypertension in mice lacking endothelial nitric oxide synthase. 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