{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T18:55:49Z","timestamp":1790189749535,"version":"4.1.0"},"reference-count":35,"publisher":"American Diabetes Association","issue":"7","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,7,1]]},"abstract":"<jats:p>To test whether pancreatic duct cells are in vitro progenitors, they were purified from dispersed islet-depleted human pancreatic tissue using CA19-9 antibody. The purified fraction was almost entirely CK19+ with no insulin+ cells, whereas the unpurified cells (crude duct) were 56% CK19+ and 0.4% insulin+ of total cells (0.7% of CK19+ cells). These cells were expanded as monolayers, aggregated under serum-free conditions, and transplanted into normoglycemic NOD\/SCID mice. In crude duct grafts, insulin+ cells increased to 6.1% of CK19+ cells. Purified duct cells had slow expansion and poor aggregation, as well as engraftment. The addition of 0.1% cultured stromal cells improved these parameters. These stromal cells contained no CK19+ cells and no insulin by either quantitative RT-PCR or immunohistochemistry; stromal cell aggregates and grafts contained no insulin+ cells. Aggregation of purified duct plus stromal preparations induced insulin+ cells (0.1% of CK19+ cells), with further increase to 1.1% in grafts. Insulin mRNA mirrored these changes. In these grafts, all insulin+ cells were in duct-like structures, while in crude duct grafts, 85% were. Some insulin+ cells coexpressed duct markers (CK19 and CA19-9) and heat shock protein (HSP)27, a marker of nonislet cells, suggesting the transition from duct. Thus, purified duct cells from adult human pancreas can differentiate to insulin-producing cells.<\/jats:p>","DOI":"10.2337\/db06-1670","type":"journal-article","created":{"date-parts":[[2007,6,27]],"date-time":"2007-06-27T16:33:45Z","timestamp":1182962025000},"page":"1802-1809","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":124,"title":["Differentiation of Affinity-Purified Human Pancreatic Duct Cells to \u03b2-Cells"],"prefix":"10.2337","volume":"56","author":[{"given":"Shigeru","family":"Yatoh","sequence":"first","affiliation":[{"name":"From the Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rikke","family":"Dodge","sequence":"additional","affiliation":[{"name":"From the Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyuki","family":"Akashi","sequence":"additional","affiliation":[{"name":"From the Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulkadir","family":"Omer","sequence":"additional","affiliation":[{"name":"From the Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arun","family":"Sharma","sequence":"additional","affiliation":[{"name":"From the Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon C.","family":"Weir","sequence":"additional","affiliation":[{"name":"From the Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Bonner-Weir","sequence":"additional","affiliation":[{"name":"From the Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","reference":[{"key":"2022031208182603800_R1","doi-asserted-by":"crossref","unstructured":"Weaver CV, Sorenson RL, Kaung HC: Immunocytochemical localization of insulin-immunoreactive cells in the ducts of rats treated with trypsin inhibitor. 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