{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T02:22:28Z","timestamp":1773454948015,"version":"3.50.1"},"reference-count":38,"publisher":"American Diabetes Association","issue":"9","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,9,1]]},"abstract":"<jats:p>Islets are composed mostly of \u03b2-cells, and therefore stem cell research has concentrated on generating purified \u03b2-cells, neglecting the other endocrine cell types in the islet. We investigated the presence of endocrine non\u2013\u03b2-cells after islet transplantation. In addition, we studied whether the transplantation of pure \u03b2-cells, in volumes similar to that used in islet transplantation, would suffice to reverse hyperglycemia in diabetic mice. Rat islets were dispersed and \u03b2-cells were purified by fluorescence-activated cell sorting according to their endogenous fluorescence. After reaggregation, 600 islet equivalents of the purified \u03b2-cell aggregates were implanted into diabetic SCID mice. In mice implanted with \u03b2-cell\u2013enriched aggregates, the hyperglycemia was reversed and good graft function over a 12-week period was observed with regard to glucose and insulin levels, glucose tolerance tests, and graft insulin content. The endocrine cell composition of the \u03b2-cell\u2013enriched aggregates remained constant; before and 12 weeks after transplantation, the \u03b2-cell\u2013enriched aggregates comprised 95% \u03b2-cells and 5% endocrine non\u2013\u03b2-cells. However, islet grafts, despite originally having comprised 75% \u03b2-cells and 25% endocrine non\u2013\u03b2-cells, comprised just 5% endocrine non\u2013\u03b2-cells after transplantation, indicating a loss of these cells. \u03b2-Cell\u2013enriched aggregates can effectively reverse hyperglycemia in mice, and transplanted intact islets are depleted in non\u2013\u03b2-cells. It is therefore likely that islet non\u2013\u03b2-cells are not essential for successful islet transplantation.<\/jats:p>","DOI":"10.2337\/db07-0191","type":"journal-article","created":{"date-parts":[[2007,8,28]],"date-time":"2007-08-28T17:28:04Z","timestamp":1188322084000},"page":"2312-2318","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":51,"title":["Normal Relationship of \u03b2- and Non\u2013\u03b2-Cells Not Needed for Successful Islet Transplantation"],"prefix":"10.2337","volume":"56","author":[{"given":"Aileen J.F.","family":"King","sequence":"first","affiliation":[{"name":"Section on Islet Transplantation and Cell Biology, Research Division, Joslin Diabetes Center, Boston, Massachusetts"},{"name":"Department of Medicine, Harvard Medical School, Boston, Massachusetts"},{"name":"Division of Reproduction and Endocrinology, Guy's Campus, King's College London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin R.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Section on Islet Transplantation and Cell Biology, Research Division, Joslin Diabetes Center, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Hollister-Lock","sequence":"additional","affiliation":[{"name":"Section on Islet Transplantation and Cell Biology, Research Division, Joslin Diabetes Center, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cameron E.","family":"Nienaber","sequence":"additional","affiliation":[{"name":"Section on Islet Transplantation and Cell Biology, Research Division, Joslin Diabetes Center, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan","family":"Bonner-Weir","sequence":"additional","affiliation":[{"name":"Section on Islet Transplantation and Cell Biology, Research Division, Joslin Diabetes Center, Boston, Massachusetts"},{"name":"Department of Medicine, Harvard Medical School, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gordon C.","family":"Weir","sequence":"additional","affiliation":[{"name":"Section on Islet Transplantation and Cell Biology, Research Division, Joslin Diabetes Center, Boston, Massachusetts"},{"name":"Department of Medicine, Harvard Medical School, Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","reference":[{"key":"2022031208203066400_R1","doi-asserted-by":"crossref","unstructured":"Baetens D, Malaisse-Lagae F, Perrelet A, Orci L: Endocrine pancreas: three-dimensional reconstruction shows two types of islets of Langerhans. 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