{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T06:53:06Z","timestamp":1789714386284,"version":"4.0.1"},"reference-count":45,"publisher":"American Diabetes Association","issue":"8","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2008,8,1]]},"abstract":"<jats:p>OBJECTIVE\u2014Engraftment and function of human islet cell implants is considered to be dependent on their rapid and adequate revascularization. Studies with rodent islet grafts have shown that vascular endothelial growth factor (VEGF) expression by \u03b2-cells can promote this process. The present work examines whether human islet preparations produce VEGF as well as interleukin (IL)-8, another angiogenic protein, and assesses the role of contaminating duct cells in VEGF and IL-8\u2013mediated angiogenesis.<\/jats:p><jats:p>RESEARCH DESIGN AND METHODS\u2014Human islet and pancreatic duct cell preparations are compared for their respective expression and production of VEGF and IL-8 during culture as well as following transplantation in nonobese diabetic (NOD)\/scid mice. The associated angiogenic effects are measured in an in vitro aortic ring assay and in an in vivo chick embryo chorioallantoic membrane assay.<\/jats:p><jats:p>RESULTS\u2014Cultured pancreatic duct cells expressed 3- and 10-fold more VEGF and IL-8, respectively, than cultured human islet endocrine cells and released both proteins at angiogenic levels. The angiogenic effect of purified duct cells was higher than that of purified endocrine islet cells and was completely blocked by a combination of IL-8 and VEGF antibodies. Human duct cell implants under the kidney capsule of NOD\/scid mice expressed higher levels of IL-8 and VEGF than human islet cell implants and induced circulating IL-8 and VEGF levels during the first day posttransplantation.<\/jats:p><jats:p>CONCLUSIONS\u2014Human duct cell\u2013released IL-8 and VEGF may help revascularization of currently used human islet cell grafts. Further work should examine whether and when this effect can prevail over other inflammatory and immune influences of this cell type.<\/jats:p>","DOI":"10.2337\/db07-1705","type":"journal-article","created":{"date-parts":[[2008,5,21]],"date-time":"2008-05-21T03:25:11Z","timestamp":1211340311000},"page":"2128-2136","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":44,"title":["Pancreatic Duct Cells in Human Islet Cell Preparations Are a Source of Angiogenic Cytokines Interleukin-8 and Vascular Endothelial Growth Factor"],"prefix":"10.2337","volume":"57","author":[{"given":"Babak","family":"Movahedi","sequence":"first","affiliation":[{"name":"Diabetes Research Center, Brussels Free University, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Conny","family":"Gysemans","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Medicine and Endocrinology, Katholieke Universiteit Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Jacobs-Tulleneers-Thevissen","sequence":"additional","affiliation":[{"name":"Diabetes Research Center, Brussels Free University, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Mathieu","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Medicine and Endocrinology, Katholieke Universiteit Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Pipeleers","sequence":"additional","affiliation":[{"name":"Diabetes Research Center, Brussels Free University, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","reference":[{"key":"2022031207585399400_R1","doi-asserted-by":"crossref","unstructured":"Ryan EA, Paty BW, Senior PA, Bigam D, Alfadhli E, Kneteman NM, Lakey JR, Shapiro AM: Five-year follow-up after clinical islet transplantation. 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