{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T21:16:59Z","timestamp":1792185419302,"version":"4.4.0"},"reference-count":43,"publisher":"American Diabetes Association","issue":"1","license":[{"start":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T00:00:00Z","timestamp":1630022400000},"content-version":"vor","delay-in-days":4349,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,1,1]]},"abstract":"<jats:sec>\n                    <jats:title>OBJECTIVE<\/jats:title>\n                    <jats:p>An emerging model of metabolic syndrome and type 2 diabetes is of adipose dysfunction with leukocyte recruitment into adipose leading to chronic inflammation and insulin resistance (IR). This study sought to explore potential mechanisms of inflammatory-induced IR in humans with a focus on adipose tissue.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESEARCH DESIGN AND METHODS<\/jats:title>\n                    <jats:p>We performed a 60-h endotoxemia protocol (3 ng\/kg intravenous bolus) in healthy adults (n = 20, 50% male, 80% Caucasian, aged 27.3 \u00b1 4.8 years). Before and after endotoxin, whole-blood sampling, subcutaneous adipose biopsies, and frequently sampled intravenous glucose tolerance (FSIGT) testing were performed. The primary outcome was the FSIGT insulin sensitivity index (Si). Secondary measures included inflammatory and metabolic markers and whole-blood and adipose mRNA and protein expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>Endotoxemia induced systemic IR as demonstrated by a 35% decrease in Si (3.17 \u00b1 1.66 to 2.06 \u00b1 0.73 \u00d7 10\u22124 [\u03bcU \u00b7 ml\u22121 \u00b7 min\u22121], P &amp;lt; 0.005), while there was no effect on pancreatic \u03b2-cell function. In adipose, endotoxemia suppressed insulin receptor substrate-1 and markedly induced suppressor of cytokine signaling proteins (1 and 3) coincident with local activation of innate (interleukin-6, tumor necrosis factor) and adaptive (monocyte chemoattractant protein-1 and CXCL10 chemokines) inflammation. These changes are known to attenuate insulin receptor signaling in model systems.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>We demonstrate, for the first time in humans, that acute inflammation induces systemic IR following modulation of specific adipose inflammatory and insulin signaling pathways. It also provides a rationale for focused mechanistic studies and a model for human proof-of-concept trials of novel therapeutics targeting adipose inflammation in IR and related consequences in humans.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2337\/db09-0367","type":"journal-article","created":{"date-parts":[[2009,9,30]],"date-time":"2009-09-30T22:34:55Z","timestamp":1254350095000},"page":"172-181","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":310,"title":["Experimental Endotoxemia Induces Adipose Inflammation and Insulin Resistance in Humans"],"prefix":"10.2337","volume":"59","author":[{"given":"Nehal N.","family":"Mehta","sequence":"first","affiliation":[{"name":"the Cardiovascular Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania;"},{"name":"the Institute for Translational Medicine and Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania;"},{"name":"Institute for Diabetes Obesity and Metabolism, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona C.","family":"McGillicuddy","sequence":"additional","affiliation":[{"name":"the Cardiovascular Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania;"},{"name":"the Institute for Translational Medicine and Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Medicine, Darthmouth Hitchcock Medical Center, Lebanon, New Hampshire;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine C.","family":"Hinkle","sequence":"additional","affiliation":[{"name":"the Cardiovascular Institute, University of Pennsylvania School of Medicine, 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