{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:35:07Z","timestamp":1791178507330,"version":"4.1.0"},"reference-count":22,"publisher":"American Diabetes Association","issue":"9","license":[{"start":{"date-parts":[[2021,8,27]],"date-time":"2021-08-27T00:00:00Z","timestamp":1630022400000},"content-version":"vor","delay-in-days":3660,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2011,9,1]]},"abstract":"<jats:sec>\n                  <jats:title>OBJECTIVE<\/jats:title>\n                  <jats:p>To investigate autoantigens in \u03b2-cells, we have used a panel of pathogenic T-cell clones that were derived from the NOD mouse. Our particular focus in this study was on the identification of the target antigen for the highly diabetogenic T-cell clone BDC-5.2.9.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESEARCH DESIGN AND METHODS<\/jats:title>\n                  <jats:p>To purify \u03b2-cell antigens, we applied sequential size exclusion chromatography and reverse-phase high-performance liquid chromatography to membrane preparations of \u03b2-cell tumors. The presence of antigen was monitored by measuring the interferon-\u03b3 production of BDC-5.2.9 in response to chromatographic fractions in the presence of NOD antigen-presenting cells. Peak antigenic fractions were analyzed by ion-trap mass spectrometry, and candidate proteins were further investigated through peptide analysis and, where possible, testing of islet tissue from gene knockout mice.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>Mass-spectrometric analysis revealed the presence of islet amyloid polypeptide (IAPP) in antigen-containing fractions. Confirmation of IAPP as the antigen target was demonstrated by the inability of islets from IAPP-deficient mice to stimulate BDC-5.2.9 in vitro and in vivo and by the existence of an IAPP-derived peptide that strongly stimulates BCD-5.2.9.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>IAPP is the target antigen for the diabetogenic CD4 T-cell clone BDC-5.2.9.<\/jats:p>\n               <\/jats:sec>","DOI":"10.2337\/db11-0288","type":"journal-article","created":{"date-parts":[[2011,7,7]],"date-time":"2011-07-07T04:19:21Z","timestamp":1310012361000},"page":"2325-2330","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":55,"title":["Islet Amyloid Polypeptide Is a Target Antigen for Diabetogenic CD4+ T Cells"],"prefix":"10.2337","volume":"60","author":[{"given":"Thomas","family":"Delong","sequence":"first","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado Denver School of Medicine and National Jewish Health, Denver, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rocky L.","family":"Baker","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado Denver School of Medicine and National Jewish Health, Denver, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nichole","family":"Reisdorph","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado Denver School of Medicine and National Jewish Health, Denver, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Reisdorph","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado Denver School of Medicine and National Jewish Health, Denver, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger L.","family":"Powell","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado Denver School of Medicine and National Jewish Health, Denver, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Armstrong","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado Denver School of Medicine and National Jewish Health, Denver, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gene","family":"Barbour","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado Denver School of Medicine and National Jewish Health, Denver, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda","family":"Bradley","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado Denver School of Medicine and National Jewish Health, Denver, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn","family":"Haskins","sequence":"additional","affiliation":[{"name":"Integrated Department of Immunology, University of Colorado Denver School of Medicine and National Jewish Health, Denver, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","published-online":{"date-parts":[[2011,8,20]]},"reference":[{"key":"2022031210223843900_B1","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1111\/j.1365-2567.2010.03362.x","article-title":"T-cell autoantigens in the non-obese diabetic mouse model of autoimmune 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