{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:37:16Z","timestamp":1791160636361,"version":"4.1.0"},"reference-count":34,"publisher":"American Diabetes Association","issue":"5","license":[{"start":{"date-parts":[[2021,8,30]],"date-time":"2021-08-30T00:00:00Z","timestamp":1630281600000},"content-version":"vor","delay-in-days":3426,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,5,1]]},"abstract":"<jats:sec>\n                  <jats:title\/>\n                  <jats:p>The activity of 6-phosphofructo-1-kinase is strictly controlled by fructose-2,6-bisphosphate, the level of which is regulated by another enzyme, 6-phosphofructo-2-kinase\/fructose-2,6-bisphosphatase (PFK2\/FBP2). PFK2\/FBP2 is a bifunctional enzyme, having kinase and phosphatase activities, and regulates both glycolysis and gluconeogenesis. Here, we examined the hormonal regulation of the PFK2\/FBP2 gene in vitro using the reporter assay, the electromobility shift assay (EMSA), and the chromatin immunoprecipitation (ChIP) assay in HuH7 cells and also using the mouse liver in vivo. We found that the transcriptional activity of the PFK2\/FBP2 gene was stimulated by insulin and inhibited by cAMP and glucocorticoid. Liver X receptor (LXR) \u03b1 showed a potent and specific stimulatory effect on PFK2\/FBP2 gene transcription. Deletion and mutagenesis analyses identified the LXR response element (LXRE) in the 5\u2032-promoter region of the PFK2\/FBP2 gene. Binding of LXR\u03b1 was confirmed by the EMSA and ChIP assay. Endogenous PFK2\/FBP2 mRNA in the mouse liver was increased in the fasting\/refeeding state compared with the fasting state. Altogether, PFK2\/FBP2 gene transcription is found to be regulated in a way that is more similar to other glycolytic enzyme genes than to gluconeogenic genes. Furthermore, our data strongly suggest that LXR\u03b1 is one of the key regulators of PFK2\/FBP2 gene transcription.<\/jats:p>\n               <\/jats:sec>","DOI":"10.2337\/db11-1255","type":"journal-article","created":{"date-parts":[[2012,3,15]],"date-time":"2012-03-15T00:18:11Z","timestamp":1331770691000},"page":"1062-1071","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":20,"title":["Liver X Receptor \u03b1 Is Involved in the Transcriptional Regulation of the 6-Phosphofructo-2-Kinase\/Fructose-2,6-Bisphosphatase Gene"],"prefix":"10.2337","volume":"61","author":[{"given":"Li-Feng","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University, Kochi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasumasa","family":"Iwasaki","sequence":"additional","affiliation":[{"name":"Health Care Center, Kochi University, Kochi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuru","family":"Nishiyama","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University, Kochi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takafumi","family":"Taguchi","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University, Kochi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Tsugita","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University, Kochi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mizuho","family":"Okazaki","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University, Kochi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuichi","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University, Kochi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Machiko","family":"Kambayashi","sequence":"additional","affiliation":[{"name":"Health Care Center, Kochi University, Kochi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimpei","family":"Fujimoto","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University, Kochi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koshi","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, Gunma, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Murao","sequence":"additional","affiliation":[{"name":"Department of Advanced Medicine, Faculty of Medicine, Kagawa University, Kagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshio","family":"Terada","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University, Kochi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","published-online":{"date-parts":[[2012,4,13]]},"reference":[{"key":"2022031223051147700_B1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/jcb.240550002","article-title":"Glucose fatty acid interactions and the regulation of glucose disposal","volume":"55","author":"Randle","year":"1994","journal-title":"J Cell 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Res"}],"container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/61\/5\/1062\/563188\/1062.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/61\/5\/1062\/563188\/1062.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:28:29Z","timestamp":1667406509000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/61\/5\/1062\/33827\/Liver-X-Receptor-Is-Involved-in-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4,13]]},"references-count":34,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2012,4,13]]},"published-print":{"date-parts":[[2012,5,1]]}},"URL":"https:\/\/doi.org\/10.2337\/db11-1255","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4,13]]}}}