{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:32:34Z","timestamp":1791181954378,"version":"4.1.0"},"reference-count":47,"publisher":"American Diabetes Association","issue":"5","license":[{"start":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T00:00:00Z","timestamp":1631059200000},"content-version":"vor","delay-in-days":3067,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,5,1]]},"abstract":"<jats:p>The existence of adult \u03b2-cell progenitors remains the most controversial developmental biology topic in diabetes research. It has been reported that \u03b2-cell progenitors can be activated by ductal ligation\u2013induced injury of adult mouse pancreas and apparently act in a cell-autonomous manner to double the functional \u03b2-cell mass within a week by differentiation and proliferation. Here, we demonstrate that pancreatic duct ligation (PDL) does not activate progenitors to contribute to \u03b2-cell mass expansion. Rather, PDL stimulates massive pancreatic injury, which alters pancreatic composition and thus complicates accurate measurement of \u03b2-cell content via traditional morphometry methodologies that superficially sample the pancreas. To overcome this potential bias, we quantified \u03b2-cells from the entire pancreas and observed that \u03b2-cell mass and insulin content are totally unchanged by PDL-induced injury. Lineage-tracing studies using sequential administration of thymidine analogs, rat insulin 2 promoter\u2013driven cre-lox, and low-frequency ubiquitous cre-lox reveal that PDL does not convert progenitors to the \u03b2-cell lineage. Thus, we conclude that \u03b2-cells are not generated in injured adult mouse pancreas.<\/jats:p>","DOI":"10.2337\/db12-0848","type":"journal-article","created":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T22:52:20Z","timestamp":1359067940000},"page":"1634-1645","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":95,"title":["\u03b2-Cells Are Not Generated in Pancreatic Duct Ligation\u2013Induced Injury in Adult Mice"],"prefix":"10.2337","volume":"62","author":[{"given":"Matthew M.","family":"Rankin","sequence":"first","affiliation":[{"name":"Division of Endocrinology and Diabetes, Children\u2019s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Wilbur","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Diabetes, Children\u2019s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly","family":"Rak","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Diabetes, Children\u2019s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily J.","family":"Shields","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Diabetes, Children\u2019s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Granger","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Diabetes, Children\u2019s Hospital of Philadelphia, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jake A.","family":"Kushner","sequence":"additional","affiliation":[{"name":"McNair Medical Institute, Pediatric Diabetes and Endocrinology, Baylor College of Medicine, Houston, Texas"},{"name":"Texas Children\u2019s Diabetes and Endocrinology Center, Texas Children\u2019s Hospital, Houston, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","published-online":{"date-parts":[[2013,4,16]]},"reference":[{"key":"2022031303180190500_B1","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1111\/j.1365-2796.2009.02156.x","article-title":"Cellular origins of beta-cell regeneration: a legacy view of historical controversies","volume":"266","author":"Granger","year":"2009","journal-title":"J Intern Med"},{"key":"2022031303180190500_B2","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.cmet.2009.12.005","article-title":"Ductal origin hypothesis of pancreatic regeneration 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