{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T15:45:58Z","timestamp":1791042358912,"version":"4.1.0"},"reference-count":48,"publisher":"American Diabetes Association","issue":"10","license":[{"start":{"date-parts":[[2021,8,28]],"date-time":"2021-08-28T00:00:00Z","timestamp":1630108800000},"content-version":"vor","delay-in-days":2902,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,10,1]]},"abstract":"<jats:p>While sporadic human genetic studies have permitted some comparisons between rodent and human pancreatic development, the lack of a robust experimental system has not permitted detailed examination of human pancreatic development. We previously developed a xenograft model of immature human fetal pancreas grafted under the kidney capsule of immune-incompetent mice, which allowed the development of human pancreatic \u03b2-cells. Here, we compared the development of human and murine fetal pancreatic grafts either under skeletal muscle epimysium or under the renal capsule. We demonstrated that human pancreatic \u03b2-cell development occurs more slowly (weeks) than murine pancreas (days) both by differentiation of pancreatic progenitors and by proliferation of developing \u03b2-cells. The superficial location of the skeletal muscle graft and its easier access permitted in vivo lentivirus-mediated gene transfer with a green fluorescent protein-labeled construct under control of the insulin or elastase gene promoter, which targeted \u03b2-cells and nonendocrine cells, respectively. This model of engraftment under the skeletal muscle epimysium is a new approach for longitudinal studies, which allows localized manipulation to determine the regulation of human pancreatic development.<\/jats:p>","DOI":"10.2337\/db13-0554","type":"journal-article","created":{"date-parts":[[2013,7,9]],"date-time":"2013-07-09T03:40:07Z","timestamp":1373341207000},"page":"3479-3487","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":22,"title":["Mouse Muscle As an Ectopic Permissive Site for Human Pancreatic Development"],"prefix":"10.2337","volume":"62","author":[{"given":"Carmen","family":"Capito","sequence":"first","affiliation":[{"name":"INSERM U845, Research Center Growth and Signalling, Facult\u00e9 de M\u00e9decine Cochin, Universit\u00e9 Paris Descartes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Th\u00e9r\u00e8se","family":"Simon","sequence":"additional","affiliation":[{"name":"INSERM U845, Research Center Growth and Signalling, Facult\u00e9 de M\u00e9decine Cochin, Universit\u00e9 Paris Descartes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Aiello","sequence":"additional","affiliation":[{"name":"INSERM U845, Research Center Growth and Signalling, Facult\u00e9 de M\u00e9decine Cochin, Universit\u00e9 Paris Descartes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Clark","sequence":"additional","affiliation":[{"name":"Diabetes Research Laboratories, Oxford Centre for Diabetes, Endocrinology and Metabolism, Churchill Hospital, Oxford, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Aigrain","sequence":"additional","affiliation":[{"name":"Necker Enfants Malades 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