{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T23:10:33Z","timestamp":1791846633950,"version":"4.3.4"},"reference-count":50,"publisher":"American Diabetes Association","issue":"2","license":[{"start":{"date-parts":[[2019,1,21]],"date-time":"2019-01-21T00:00:00Z","timestamp":1548028800000},"content-version":"vor","delay-in-days":69,"URL":"http:\/\/www.diabetesjournals.org\/content\/license"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["K08-DK-100534"],"award-info":[{"award-number":["K08-DK-100534"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["R01-DK-103534"],"award-info":[{"award-number":["R01-DK-103534"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006280","name":"Urology Care Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006280","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,2,1]]},"abstract":"<jats:p>The NLRP3 inflammasome senses diabetic metabolites and initiates inflammation implicated in diabetic complications and neurodegeneration. No studies have investigated NLRP3 in diabetic bladder dysfunction (DBD), despite a high clinical prevalence. In vitro, we found that numerous diabetic metabolites activate NLRP3 in primary urothelial cells. In vivo, we demonstrate NLRP3 is activated in urothelia from a genetic type 1 diabetic mouse (Akita) by week 15. We then bred an NLRP3\u2212\/\u2212 genotype into these mice and found this blocked bladder inflammation and cystometric markers of DBD. Analysis of bladder innervation established an NLRP3-dependent decrease in overall nerve density and A\u03b4-fibers in the bladder wall along with an increase in C-fiber populations in the urothelia, which potentially explains the decreased sense of bladder fullness reported by patients and overactivity detected early in DBD. Together, the results demonstrate the role of NLRP3 in the genesis of DBD and suggest specific NLRP3-mediated neuronal changes can produce specific DBD symptoms.<\/jats:p>","DOI":"10.2337\/db18-0845","type":"journal-article","created":{"date-parts":[[2018,11,13]],"date-time":"2018-11-13T11:06:04Z","timestamp":1542107164000},"page":"430-440","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":29,"title":["NLRP3 Promotes Diabetic Bladder Dysfunction and Changes in Symptom-Specific Bladder Innervation"],"prefix":"10.2337","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3776-3653","authenticated-orcid":false,"suffix":"Jr.","given":"Francis M.","family":"Hughes","sequence":"first","affiliation":[{"name":"Department of Surgery, Division of Urology, Duke University Medical Center, Durham, NC"},{"name":"Department of Bioengineering, Clemson University, Clemson, SC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan A.","family":"Hirshman","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Urology, Duke University Medical Center, Durham, NC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian M.","family":"Inouye","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Urology, Duke University Medical Center, Durham, NC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huixia","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Urology, Duke University Medical Center, Durham, NC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eloise W.","family":"Stanton","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Urology, Duke University Medical Center, Durham, NC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chloe E.","family":"Yun","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Urology, Duke University Medical Center, Durham, NC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leah G.","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Urology, Duke University Medical Center, Durham, NC"},{"name":"Duke Cancer Center Biostatistics, Duke University Medical Center, Durham, NC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan C.","family":"Routh","sequence":"additional","affiliation":[{"name":"Department of Surgery, Division of Urology, Duke University Medical Center, Durham, NC"},{"name":"Department of Pediatrics, Duke University Medical Center, Durham, NC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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