{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:41:59Z","timestamp":1791319319994,"version":"4.3.1"},"reference-count":49,"publisher":"American Diabetes Association","issue":"2","license":[{"start":{"date-parts":[[2021,1,20]],"date-time":"2021-01-20T00:00:00Z","timestamp":1611100800000},"content-version":"vor","delay-in-days":76,"URL":"https:\/\/www.diabetesjournals.org\/content\/license"}],"funder":[{"DOI":"10.13039\/501100001659","name":"DFG","doi-asserted-by":"publisher","award":["DR225\/11-1"],"award-info":[{"award-number":["DR225\/11-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,2,1]]},"abstract":"<jats:p>The aim for this study was to elucidate how the hypothalamic hunger-inducing hormone acyl-ghrelin (AG), which is also produced in the pancreas, affects \u03b2-cell function, with particular attention to the role of ATP-sensitive K+ (KATP) channels and the exact site of action of the hormone. AG hyperpolarized the membrane potential and decreased cytoplasmic calcium concentration [Ca2+]c and glucose-stimulated insulin secretion (GSIS). These effects were abolished in \u03b2-cells from SUR1-knockout (KO) mice. AG increased KATP current but only in a configuration with intact metabolism. Unacylated ghrelin counteracted the effects of AG. The influence of AG on membrane potential and GSIS could only be averted in the combined presence of a ghrelin receptor (GHSR1a) antagonist and an inverse agonist. The inhibition of GSIS by AG could be prevented by dibutyryl cyclic\u2013cAMP or 3-isobutyl-1-methylxanthine and the somatostatin (SST) receptor 2\u20135 antagonist H6056. These data indicate that AG indirectly opens KATP channels probably by interference with the cAMP\/cAMP-dependent protein kinase pathway, resulting in a decrease of [Ca2+]c and GSIS. The experiments with SUR1-KO \u03b2-cells point to a direct effect of AG on \u03b2-cells and not, as earlier suggested, to an exclusive effect by AG-induced SST release from \u03b4-cells. Nevertheless, SST receptors may be involved in the effect of AG, possibly by heteromerization of AG and SST receptors.<\/jats:p>","DOI":"10.2337\/db20-0231","type":"journal-article","created":{"date-parts":[[2020,11,6]],"date-time":"2020-11-06T03:10:19Z","timestamp":1604632219000},"page":"423-435","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":17,"title":["Acyl-Ghrelin Influences Pancreatic \u03b2-Cell Function by Interference with KATP Channels"],"prefix":"10.2337","volume":"70","author":[{"given":"Julia","family":"Kaiser","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Institute of Pharmacy, University of T\u00fcbingen, T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8968-0716","authenticated-orcid":false,"given":"Peter","family":"Krippeit-Drews","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, 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