{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T12:21:34Z","timestamp":1791807694304,"version":"4.3.4"},"reference-count":36,"publisher":"American Diabetes Association","issue":"9","license":[{"start":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T00:00:00Z","timestamp":1685491200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.diabetesjournals.org\/journals\/pages\/license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DK127236"],"award-info":[{"award-number":["R01DK127236"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004329","name":"Javna Agencija za Raziskovalno Dejavnost RS","doi-asserted-by":"publisher","award":["P3-0396"],"award-info":[{"award-number":["P3-0396"]}],"id":[{"id":"10.13039\/501100004329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["I3562-B27and I4319-B30"],"award-info":[{"award-number":["I3562-B27and I4319-B30"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,9,1]]},"abstract":"<jats:sec>\n                    <jats:title\/>\n                    <jats:p>The mechanisms accounting for the functional changes of \u03b1- and \u03b2-cells over the course of type 1 diabetes (T1D) development are largely unknown. Permitted by our established technology of high spatiotemporal resolution imaging of cytosolic Ca2+ ([Ca2+]c) dynamics on fresh pancreas tissue slices, we tracked the [Ca2+]c dynamic changes, as the assessment of function, in islet \u03b1- and \u03b2-cells of female nonobese diabetic (NOD) mice during the development of spontaneous diabetes. We showed that, during the phases of islet inflammation, 8 mmol\/L glucose-induced synchronized short [Ca2+]c events in \u03b2-cells were diminished, whereas long [Ca2+]c events were gradually more triggerable at substimulatory 4 and 6 mmol\/L glucose. In the islet destruction phase, the synchronized short [Ca2+]c events in a subset of \u03b2-cells resumed at high glucose condition, while the long [Ca2+]c events were significantly elevated already at substimulatory glucose concentrations. In the \u03b1-cells, the glucose sensitivity of the [Ca2+]c events persisted throughout the course of T1D development. At the late islet destruction phase, the \u03b1-cell [Ca2+]c events exhibited patterns of synchronicity. Our work has uncovered windows of functional recovery in \u03b2-cells and potential \u03b1-cells functional synchronization in NOD mice over the course of T1D development.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Article Highlights<\/jats:title>\n                    <jats:p>In NOD mice \u03b2-cells, 8 mmol\/L glucose\u2013induced synchronized short [Ca2+]c events diminish in the early phases of islet inflammation, and long Ca2+ events became more sensitive to substimulatory 4 and 6 mmol\/L glucose. In the late islet destruction phase, the synchronized short [Ca2+]c events in a subset of \u03b2-cells resumed at 8 mmol\/L glucose, while the long Ca2+ events were significantly elevated at substimulatory glucose concentrations. In the \u03b1-cells, the glucose sensitivity of the [Ca2+]c events persisted throughout the course of type 1 diabetes development. \u03b1-Cell [Ca2+]c events occasionally synchronize in the islets with severe \u03b2-cell destruction.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2337\/db22-0952","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T15:01:12Z","timestamp":1685545272000},"page":"1251-1261","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":15,"title":["Tracking Ca2+ Dynamics in NOD Mouse Islets During Spontaneous Diabetes Development"],"prefix":"10.2337","volume":"72","author":[{"given":"Sandra","family":"Posti\u0107","sequence":"first","affiliation":[{"name":"1Center for Physiology and Pharmacology, Medical University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Pfabe","sequence":"additional","affiliation":[{"name":"1Center for Physiology and Pharmacology, Medical University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srdjan","family":"Sarikas","sequence":"additional","affiliation":[{"name":"1Center for Physiology and Pharmacology, Medical University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Ehall","sequence":"additional","affiliation":[{"name":"2Division of Endocrinology and Diabetology, Department of Internal Medicine, Medical University of Graz, Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Pieber","sequence":"additional","affiliation":[{"name":"2Division of Endocrinology and Diabetology, Department of Internal Medicine, Medical University of Graz, Graz, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dean","family":"Koro\u0161ak","sequence":"additional","affiliation":[{"name":"3Faculty of Civil Engineering, Transportation Engineering and Architecture, University of Maribor, Maribor, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjan","family":"Slak Rupnik","sequence":"additional","affiliation":[{"name":"1Center for Physiology and Pharmacology, Medical University of Vienna, Vienna, Austria"},{"name":"3Faculty of Civil Engineering, Transportation Engineering and Architecture, University of Maribor, Maribor, Slovenia"},{"name":"4Alma Mater Europaea \u2013 European Center Maribor, Maribor, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5168-1174","authenticated-orcid":false,"given":"Ya-Chi","family":"Huang","sequence":"additional","affiliation":[{"name":"1Center for Physiology and Pharmacology, Medical University of Vienna, 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