{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T02:40:59Z","timestamp":1698201659535},"reference-count":0,"publisher":"American Diabetes Association","issue":"11","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1975,11,1]]},"abstract":"<jats:p>The exchange of 125I-insulin, 125I-glucagon, 125I-proinsulin, 125I-growth hormone, 131I-albumin, 14C-inulin, and 14C-dextran across isolated rat mesentery was studied in a diffusion cell. The passage of immunoprecipitable porcine 125I-insulin (0.88 ng.\/ml.) was not affected by porcine proinsulin (145 ng.\/ml.), crystalline porcine insulin (17.4 ng.\/ml.), human growth hormone (87 ng.\/ml.), bovine serum albumin (4.5 mg.\/ml.), or normal guinea pig serum (840 \u03bcg. protein\/ml.). However, the rate of insulin exchange was reduced by guinea pig anti-insulin antiserum and partially purified human serum-bound insulin (175 \u03bcg. protein\/ml.). Bound insulin at the same concentration did not affect the exchange of 125I-glucagon, 125I-growth hormone, 14C-inulin, or 14C-dextran. Further purification of bound insulin by Sephadex G-100 chromatography yielded an approximately 45,000 molecular-weight fraction that at 5 \u03bcg. protein permilliliter allowed essentially no insulin transport. This same fraction of bound insulin significantly inhibited the disappearance of immunoprecipitable porcine 125I-insulin from the incubation medium of isolated rat hemidiaphragms. Theses studies suggest that the transport of insulin across biologic membranes, mesothelium, and possible endothelium is specifically inhibited by bound insulin, a circulating macromolecule that possesses insulin-like activity.<\/jats:p>","DOI":"10.2337\/diab.24.11.997","type":"journal-article","created":{"date-parts":[[2013,9,19]],"date-time":"2013-09-19T17:13:09Z","timestamp":1379610789000},"page":"997-1004","update-policy":"http:\/\/dx.doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":2,"title":["Transport of Peptide Hormones Across Isolated Rat Mesentery: Effect of Human Serum-Bound Insulin"],"prefix":"10.2337","volume":"24","author":[{"given":"James D","family":"Simon","sequence":"first","affiliation":[{"name":"Center for Blood Research and Department of Nutrition, Harvard School of Public Health\u2008Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harry N","family":"Antoniades","sequence":"additional","affiliation":[{"name":"Center for Blood Research and Department of Nutrition, Harvard School of Public Health\u2008Boston, Massachusetts 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/24\/11\/997\/347902\/24-11-997.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/24\/11\/997\/347902\/24-11-997.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:24:54Z","timestamp":1667406294000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/24\/11\/997\/4609\/Transport-of-Peptide-Hormones-Across-Isolated-Rat"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,11,1]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1975,11,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diab.24.11.997","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1975,11,1]]}}}