{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T15:32:43Z","timestamp":1703691163710},"reference-count":0,"publisher":"American Diabetes Association","issue":"3","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1982,3,1]]},"abstract":"<jats:p>Human fetal pancreatic glands were obtained from 12 consecutive prostaglandin-induced abortions. Explants cultured for 1 day were frozen at 0.3\u00b0C\/min in a 1 M DMSO-containing medium and stored at \u2212196\u00b0C. After storage for 3\u20134 mo the frozen material was rapidly thawed and cultured 1 day before being tested for functional performance. There was a positive correlation between the pancreatic insulin content and the fetal crown-heel length. Seven of the twelve fetuses showed a marked insulin response to an acute glucose-theophylline challenge. In five of these pancreases there was a well-preserved morphology after thawing, whereas only one of the nonresponding preparations showed a satisfactory morphology. Pancreatic explants from three of four fetuses tested displayed evidences of an (pro)insulin biosynthesis. The combined results indicated that low-temperature storage of human fetal endocrine pancreas is compatible with specific functional survival.<\/jats:p>","DOI":"10.2337\/diab.31.3.238","type":"journal-article","created":{"date-parts":[[2013,9,19]],"date-time":"2013-09-19T17:16:02Z","timestamp":1379610962000},"page":"238-241","update-policy":"http:\/\/dx.doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":2,"title":["Preservation of Morphology, Insulin Biosynthesis, and Insulin Release in Cryopreserved Human Fetal Pancreas"],"prefix":"10.2337","volume":"31","author":[{"given":"S","family":"Sandler","sequence":"first","affiliation":[{"name":"Department of Medical Cell Biology, University of Uppsala\u2008Uppsala\u2008Department of Surgery, Huddinge Hospital\u2008Huddinge, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Andersson","sequence":"additional","affiliation":[{"name":"Department of Medical Cell Biology, University of Uppsala\u2008Uppsala\u2008Department of Surgery, Huddinge Hospital\u2008Huddinge, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Hellerstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Medical Cell Biology, University of Uppsala\u2008Uppsala\u2008Department of Surgery, Huddinge Hospital\u2008Huddinge, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Petersson","sequence":"additional","affiliation":[{"name":"Department of Medical Cell Biology, University of Uppsala\u2008Uppsala\u2008Department of Surgery, Huddinge Hospital\u2008Huddinge, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Swenne","sequence":"additional","affiliation":[{"name":"Department of Medical Cell Biology, University of Uppsala\u2008Uppsala\u2008Department of Surgery, Huddinge Hospital\u2008Huddinge, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Bj\u00f6rk\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Medical Cell Biology, University of Uppsala\u2008Uppsala\u2008Department of Surgery, Huddinge Hospital\u2008Huddinge, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C -G","family":"Groth","sequence":"additional","affiliation":[{"name":"Department of Medical Cell Biology, University of Uppsala\u2008Uppsala\u2008Department of Surgery, Huddinge Hospital\u2008Huddinge, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/31\/3\/238\/351912\/31-3-238.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/31\/3\/238\/351912\/31-3-238.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:37:58Z","timestamp":1667407078000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/31\/3\/238\/5458\/Preservation-of-Morphology-Insulin-Biosynthesis"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1982,3,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diab.31.3.238","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,3,1]]}}}