{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:40:31Z","timestamp":1758818431974},"reference-count":0,"publisher":"American Diabetes Association","issue":"11","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1983,11,1]]},"abstract":"<jats:p>Anti-islet immune reactions were studied in vitro in genetically diabetic homozygote C57BL\/KsJ db\/db mice, using murine islet cells as a target. Spleen lymphocytes inhibited insulin secretion by the islet cells. This inhibition was abolished when T-cells were eliminated by treatment with anti-Thy 1.2 monoclonal antibody in the presence of complement. Anti-islet complement-dependent antibody (CDA) and antibody-dependent cell cytotoxicity (ADCC) were also found in the sera of these mice. This anti-islet immunity was detectable as early as the tenth day of life and lasted throughout the entire life span of the animals. A significant lymphopenia was detected in thymus and spleen cell populations. None of these anomalies was found in control heterozygote mice.<\/jats:p>\n               <jats:p>Thymic function was explored in the same mice by evaluating their serum thymic factor (FTS) levels using a rosette assay. The age-dependent decline of FTS levels was significantly accelerated in diabetic mice as compared with heterozygous littermates. Futhermore, FTS inhibitory immunoglobulins were detected in db\/db mouse sera, which inactivated in vitro the biologic potency of synthetic FTS. Histologically, the thymus displayed an accelerated involution. It was shown by indirect immunofluorescence using anti-FTS monoclonal antibodies that the number of FTS+ cells was reduced in db\/db mouse thymuses.<\/jats:p>\n               <jats:p>Histologie study of the islets of Langerhans showed early signs of \u03b2-cell hyperactivity and hypertrophy, followed by \u03b2-cell rarefaction and profound dislocation of islet architecture. Insulitis was not detected.<\/jats:p>","DOI":"10.2337\/diab.32.11.1048","type":"journal-article","created":{"date-parts":[[2013,9,19]],"date-time":"2013-09-19T17:16:02Z","timestamp":1379610962000},"page":"1048-1054","update-policy":"http:\/\/dx.doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":13,"title":["Anti-Islet Immunity and Thymic Dysfunction in the Mutant Diabetic C57BL\/ KsJ <i>db<\/i>\/<i>db<\/i> Mouse"],"prefix":"10.2337","volume":"32","author":[{"given":"Monique","family":"Debray-Sachs","sequence":"first","affiliation":[{"name":"INSERM U 25, H\u00f4pital Necker\u2008Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mireille","family":"Dardenne","sequence":"additional","affiliation":[{"name":"INSERM U 25, H\u00f4pital Necker\u2008Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Sai","sequence":"additional","affiliation":[{"name":"Ecole V\u00e9t\u00e9rinaire\u2008Nantes, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilson","family":"Savino","sequence":"additional","affiliation":[{"name":"INSERM U 25, H\u00f4pital Necker\u2008Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Christine","family":"Quiniou","sequence":"additional","affiliation":[{"name":"Diabetes Department, H\u00f4pital Bichat\u2008Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Boillot","sequence":"additional","affiliation":[{"name":"Diabetes Department, H\u00f4pital Bichat\u2008Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Willy","family":"Gepts","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology VUB\u2008Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Assan","sequence":"additional","affiliation":[{"name":"Diabetes Department, H\u00f4pital Bichat\u2008Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/32\/11\/1048\/352476\/32-11-1048.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/32\/11\/1048\/352476\/32-11-1048.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:39:23Z","timestamp":1667407163000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/32\/11\/1048\/5696\/Anti-Islet-Immunity-and-Thymic-Dysfunction-in-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,11,1]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1983,11,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diab.32.11.1048","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,11,1]]}}}