{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:28:54Z","timestamp":1760189334894},"reference-count":0,"publisher":"American Diabetes Association","issue":"8","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1986,8,1]]},"abstract":"<jats:p>The direct effect of insulin on the high-affinity Ca2+-Mg2+-ATPase was studied in kidney proximal tubular basolateral membranes (BLM) obtained from control and streptozocin-induced non-insulin-dependent diabetes mellitus (NIDDM) rats. Plasma glucose of the diabetic animals was only mildly elevated (217 \u00b1 9 vs. 138 \u00b1 3 mg\/dl). Both high- and low-affinity calcium-dependent Ca2+-Mg2+-ATPase activities were identified in the BLM. Enzyme activity in BLM from diabetic rats was higher at all Ca2+ concentrations tested due to a higher maximum velocity of the enzyme from NIDDM rats. The high-affinity Ca2+-Mg2+-ATPase activity was inhibited by trifluoroperzine (TFP) in both membranes. No difference in calmodulin content was found in the membranes from the diabetic and control rats.<\/jats:p>\n               <jats:p>Insulin (16\u2013200 \u03bcU\/ml) significantly increased the high-affinity Ca2+-Mg2+-ATPase activity (17\u201340%) in membranes from control animals but had no effect on the enzyme activity in the membranes from the NIDDM rats. The basal activity of the enzyme at 0.1 \u03bcM free Ca2+ was higher in the BLM from the NIDDM animals compared to controls (17.8 \u00b1 0.5 vs. 14.7 \u00b1 0.8 nM Pi mg1 \u00b7 min\u22121; P &amp;lt; .02). There was no effect of insulin on the Ca2+-independent ATPase activity of BLM preparations.<\/jats:p>\n               <jats:p>These findings demonstrate a defect in the ability of insulin to regulate the high-affinity Ca2+-Mg2+-ATPase activity in BLM from diabetic rats. Such a defect in enzyme activity may play a role in the mechanism of impaired insulin action observed in these NIDDM rats.<\/jats:p>","DOI":"10.2337\/diab.35.8.899","type":"journal-article","created":{"date-parts":[[2013,9,19]],"date-time":"2013-09-19T17:16:02Z","timestamp":1379610962000},"page":"899-905","update-policy":"http:\/\/dx.doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":20,"title":["Ca2+-Mg2+-ATPase Activity in Kidney Basolateral Membrane in Non-Insulin-Dependent Diabetic Rats: Effect of Insulin"],"prefix":"10.2337","volume":"35","author":[{"given":"J","family":"Levy","sequence":"first","affiliation":[{"name":"Division of Endocrinology and Bone Metabolism, The Jewish Hospital of St. Louis, and Metabolism Division, Washington University School of Medicine\u2008St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"J R","family":"Gavin","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Bone Metabolism, The Jewish Hospital of St. Louis, and Metabolism Division, Washington University School of Medicine\u2008St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M R","family":"Hammerman","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Bone Metabolism, The Jewish Hospital of St. Louis, and Metabolism Division, Washington University School of Medicine\u2008St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L V","family":"Avioli","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Bone Metabolism, The Jewish Hospital of St. Louis, and Metabolism Division, Washington University School of Medicine\u2008St. Louis, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/35\/8\/899\/354788\/35-8-899.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/35\/8\/899\/354788\/35-8-899.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:46:22Z","timestamp":1667407582000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/35\/8\/899\/6355\/Ca2-Mg2-ATPase-Activity-in-Kidney-Basolateral"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,8,1]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1986,8,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diab.35.8.899","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,8,1]]}}}