{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T04:29:37Z","timestamp":1667449777201},"reference-count":0,"publisher":"American Diabetes Association","issue":"2","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1988,2,1]]},"abstract":"<jats:p>Glucose, forskolin, 3-isobutyl-1-methylxanthine (IBMX), and dexamethasone were tested as regulators of proinsulin biosynthesis in HIT T-15 cells, which are glucose-responsive simian virus 40-transformed hamster \u03b2-cells. Rate of [3H]leucine incorporation into proinsulin was increased as glucose concentrations were raised from 0 to 20 mM. Biosynthetic rate increases were significant after 48 but not at 4 or 24 h of glucose and were greater for proinsulin than for total extractable proteins. After 48 h, glucose-stimulated proinsulin biosynthesis was unaffected by 10\u22126 M forskolin and\/or 3 \u00d7 10\u22125 M IBMX but was specifically and significantly inhibited by 10\u22126 M dexamethasone. Four hours of exposure to dexamethasone had no effect. When cells were incubated for 24 h and then continuously labeled for an additional 24 h, cellular conversion of labeled proinsulin to insulin was increased by glucose, and this increase was reversed or inhibited by 10\u22126 M dexamethasone. Therefore, proinsulin biosynthesis in transformed HIT T-15 cells is regulated in several ways by metabolites and hormones in a manner that compares with biosynthetic regulation in normal \u03b2-cells.<\/jats:p>","DOI":"10.2337\/diab.37.2.160","type":"journal-article","created":{"date-parts":[[2013,9,19]],"date-time":"2013-09-19T17:16:02Z","timestamp":1379610962000},"page":"160-165","update-policy":"http:\/\/dx.doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":8,"title":["Insulin Biosynthesis in HIT Cells: Effects of Glucose, Forskolin, IBMX, and Dexamethasone"],"prefix":"10.2337","volume":"37","author":[{"given":"Gerald","family":"Gold","sequence":"first","affiliation":[{"name":"Metabolic Research Unit, University of California\u2008San Francisco, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong-Li","family":"Qian","sequence":"additional","affiliation":[{"name":"Metabolic Research Unit, University of California\u2008San Francisco, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerold M","family":"Grodsky","sequence":"additional","affiliation":[{"name":"Metabolic Research Unit, University of California\u2008San Francisco, California"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/37\/2\/160\/512903\/37-2-160.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/37\/2\/160\/512903\/37-2-160.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:08:37Z","timestamp":1667405317000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/37\/2\/160\/6710\/Insulin-Biosynthesis-in-HIT-Cells-Effects-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1988,2,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diab.37.2.160","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,2,1]]}}}