{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T08:37:59Z","timestamp":1792571879035,"version":"4.5.0"},"reference-count":0,"publisher":"American Diabetes Association","issue":"5","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,5,1]]},"abstract":"<jats:p>Islet amyloid polypeptide (IAPP) has been identified as the major constituent of the pancreatic amyloid of non-insulin-dependent diabetes mellitus (NIDDM) and is also present in normal \u03b2-cell secretory granules. To determine whether IAPP is a pancreatic secretory product, we measured the quantity of lAPP-like immunoreactivity (IAPP-LI), insulin, and glucagon released into 5 ml of incubation medium during a 2-h incubation of monolayer cultures (n = 5) of neonatal (3- to 5-day-old) Sprague-Dawley rat pancreases under three conditions: 1.67 mM glucose, 16.7 mM glucose, and 16.7 mM glucose plus 10 mM arginine and 0.1 mM isobutylmethylxanthine (IBMX). The quantity of IAPP-LI, insulin, and glucagon in the cell extract was also determined. Mean \u00b1 SE IAPP-LI in the incubation medium increased from 0.041 \u00b1 0.003 pmol in 1.67 mM glucose to 0.168 \u00b1 0.029 pmol in 16.7 mM glucose (P &amp;lt; 0.05) and 1.02 \u00b1 0.06 pmol in 16.7 mM glucose plus arginine and IBMX (P &amp;lt; 0.05 vs. 1.67 or 16.7 mM glucose). Insulin secretion increased similarly from 4.34 \u00b1 0.27 to 20.2 \u00b1 0.6 pmol (P &amp;lt; 0.05) and then to 135 \u00b1 5 pmol (P &amp;lt; 0.05 vs. 1.67 or 16.7 mM glucose). Glucagon release tended to decrease with the increase in glucose concentration (0.39 \u00b1 0.01 vs. 0.33 \u00b1 0.02 pmol, P &amp;lt; 0.1), whereas with the addition of arginine and IBMX to high glucose, glucagon release increased to 1.32 \u00b1 0.03 pmol (P &amp;lt; 0.05 vs. 1.67 or 16.7 mM glucose). Thus, the molar proportion of IAPP-LI to insulin secreted in low glucose was \u223c1% and did not differ significantly with stimulation (0.95 \u00b1 0.08 vs. 0.84 \u00b1 0.15 vs. 0.76 \u00b1 0.05%). In contrast, there was no constant proportional relationship between the release of IAPP-LI and glucagon (10.6 \u00b1 0.8 vs. 51.3 \u00b1 8.7 vs. 77.5 \u00b1 5.2%). After incubation in 1.67 mM glucose, the extracted cells contained 3.7 \u00b1 0.2 pmol IAPP-LI, 944 \u00b1 25 pmol insulin, and 28.2 \u00b11.5 pmol glucagon. After maximal stimulation, the fractional release of IAPP-LI was 26.7 \u00b1 0.7% vs. 14.7 \u00b1 0.6% of insulin and 4.4 \u00b1 0.2% of glucagon. These data indicate that nondiabetic neonatal rat islet cultures contain IAPP-LI and release it after stimulation by glucose and nonglucose secretagogues. Furthermore, the data suggest that IAPP-LI is a product of the \u03b2-cell, which coreleases it with insulin in a molar ratio of \u223c1.100.<\/jats:p>","DOI":"10.2337\/diab.39.5.634","type":"journal-article","created":{"date-parts":[[2013,9,19]],"date-time":"2013-09-19T17:22:06Z","timestamp":1379611326000},"page":"634-638","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":349,"title":["Evidence of Cosecretion of Islet Amyloid Polypeptide and Insulin by \u03b2-Cells"],"prefix":"10.2337","volume":"39","author":[{"given":"Steven E","family":"Kahn","sequence":"first","affiliation":[{"name":"Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, University of Washington and Veterans Affairs Medical Center\u2008Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A","family":"D'Alessio","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, University of Washington and Veterans Affairs Medical Center\u2008Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael W","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, University of Washington and Veterans Affairs Medical Center\u2008Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilfred Y","family":"Fujimoto","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, University of Washington and Veterans Affairs Medical Center\u2008Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W","family":"Ensinck","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, University of Washington and Veterans Affairs Medical Center\u2008Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Gerald J","family":"Taborsky","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, University of Washington and Veterans Affairs Medical Center\u2008Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Daniel","family":"Porte","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, University of Washington and Veterans Affairs Medical Center\u2008Seattle, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/39\/5\/634\/357543\/39-5-634.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/39\/5\/634\/357543\/39-5-634.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:14:34Z","timestamp":1667405674000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/39\/5\/634\/7401\/Evidence-of-Cosecretion-of-Islet-Amyloid"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1990,5,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diab.39.5.634","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,5,1]]}}}