{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T22:48:43Z","timestamp":1771627723079,"version":"3.50.1"},"reference-count":0,"publisher":"American Diabetes Association","issue":"2","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1993,2,1]]},"abstract":"<jats:p>IAPP, or amylin, is a 37-amino acid peptide that is co-secreted with insulin from the pancreatic \u03b2-cells. We have determined the effects of IAPP and the antagonist 8\u201337 fragment of IAPP on the secretion of insulin from isolated rat islets studied in a perifusion system. Insulin secretion was stimulated by 8 mM glucose and 0.2 \u03bcM carbachol. IAPP at 10\u22127 M reduced insulin release by 32% from 7.1 (95% Cl 5.8\u20138.6) to 4.8 (3.0\u20137.5) fmol \u00b7 min\u22121 \u00b7 islet\u22121 (P = 0.046, n = 7). IAPP at 1.5 \u00b7 10\u22126 M reduced insulin release by 62% from 6.5 (3.4\u201312.3) to 2.5 (1.4\u20134.4) fmol \u00b7 min\u22121 \u00b7 islet\u22121 (P = 0.001, n = 6). IAPP at 10\u22125 M decreased insulin release by 70% (P &amp;lt; 0.001, n = 6). When IAPP(8\u201337) at 10\u22125 M was added to IAPP at 1.5 \u00b7 10\u22126 M, there was only a 22% reduction of insulin release (P = 0.06, n = 6) compared with control chambers with ho peptide added. This reduction was less (P = 0.002) than observed with IAPP (1.5 \u00b7 10\u22126 M) alone. IAPP(8\u201337) at 4 \u00b7 10\u22125 M in the absence of exogenously added IAPP increased insulin secretion by 48% (P = 0.01, n = 6), but IAPP(8\u201337) at 10\u22125 M did not alter insulin secretion. These findings demonstrate that IAPP decreases insulin secretion from islet \u03b2-cells, an effect that can be antagonized by the 8\u201337 fragment of IAPP. The ability of the 8\u201337 fragment of IAPP alone to increase insulin secretion suggests the possibility that endogenously released IAPP has a tonic inhibitory effect on insulin secretion under the conditions tested.<\/jats:p>","DOI":"10.2337\/diab.42.2.330","type":"journal-article","created":{"date-parts":[[2013,9,19]],"date-time":"2013-09-19T17:22:06Z","timestamp":1379611326000},"page":"330-335","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":52,"title":["Influence of Islet Amyloid Polypeptide and the 8\u201337 Fragment of Islet Amyloid Polypeptide on Insulin Release From Perifused Rat Islets"],"prefix":"10.2337","volume":"42","author":[{"given":"Zhi-Li","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Medicine, Royal Postgraduate Medical School\u2008London\u2008Hemostasis Research Group, Clinical Research Center\u2008Harrow, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William M","family":"Bennet","sequence":"additional","affiliation":[{"name":"Department of Medicine, Royal Postgraduate Medical School\u2008London\u2008Hemostasis Research Group, Clinical Research Center\u2008Harrow, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad A","family":"Ghatei","sequence":"additional","affiliation":[{"name":"Department of Medicine, Royal Postgraduate Medical School\u2008London\u2008Hemostasis Research Group, Clinical Research Center\u2008Harrow, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter G H","family":"Byfield","sequence":"additional","affiliation":[{"name":"Department of Medicine, Royal Postgraduate Medical School\u2008London\u2008Hemostasis Research Group, Clinical Research Center\u2008Harrow, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Medicine, Royal Postgraduate Medical School\u2008London\u2008Hemostasis Research Group, Clinical Research Center\u2008Harrow, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen R","family":"Bloom","sequence":"additional","affiliation":[{"name":"Department of Medicine, Royal Postgraduate Medical School\u2008London\u2008Hemostasis Research Group, Clinical Research Center\u2008Harrow, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/42\/2\/330\/359877\/42-2-330.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/42\/2\/330\/359877\/42-2-330.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:23:07Z","timestamp":1667406187000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/42\/2\/330\/9982\/Influence-of-Islet-Amyloid-Polypeptide-and-the-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1993,2,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diab.42.2.330","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,2,1]]}}}