{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T20:34:22Z","timestamp":1759178062677},"reference-count":0,"publisher":"American Diabetes Association","issue":"2","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,2,1]]},"abstract":"<jats:p>The insulin receptors from erythrocytes of 50 patients with non-insulin-dependent diabetes mellitus were tested for their ability to autophosphorylate. The assay was performed by a new enzyme-linked immunosorbent assay system that used monoclonal anti-insulin receptor antibodies absorbed to microtiter plates as a first antibody and polyclonal antiphosphotyrosine antibody as a labeled second antibody. By this assay, 3 patients were identified with defects in their insulin receptor kinase, although their defects appeared heterogeneous. Patient 1 had 85% less maximal autophosphorylation with a normal ED50 (1.6 \u00d7 10\u22129 M insulin). Patient 2, who had polycystic ovary disease, had a 49.2% decrease in maximal autophosphorylation of insulin receptors, and the ED50 was shifted to the right (5.6 \u00d7 10\u22128 M). Patient 3 with acanthosis nigricans had a normal maximal autophosphorylation, but the ED50 shifted to the right (2.9 \u00d7 10\u22128 M). The mechanisms for the diversity detected in this assay is not known, but this technique has sufficient specificity and sensitivity to be used to screen for insulin-resistant patients who have a lack of kinase activity.<\/jats:p>","DOI":"10.2337\/diab.43.2.274","type":"journal-article","created":{"date-parts":[[2013,9,19]],"date-time":"2013-09-19T17:22:06Z","timestamp":1379611326000},"page":"274-280","update-policy":"http:\/\/dx.doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":11,"title":["Enzyme-Linked Immunosorbent Assay Method for Human Autophosphorylated Insulin Receptor: Applicability to Insulin-Resistant States"],"prefix":"10.2337","volume":"43","author":[{"given":"Haruhiko","family":"Hagino","sequence":"first","affiliation":[{"name":"Hyogo Institute for Clinical Research\u2008Akashi"},{"name":"Department of Internal Medicine, Kobe University School of Medicine\u2008Kobe"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kozui","family":"Shii","sequence":"additional","affiliation":[{"name":"Hyogo Institute for Clinical Research\u2008Akashi"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Yokono","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Kobe University School of Medicine\u2008Kobe"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Matsuba","sequence":"additional","affiliation":[{"name":"Hyogo Institute for Clinical Research\u2008Akashi"},{"name":"Department of Internal Medicine, Kobe University School of Medicine\u2008Kobe"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaki","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Hyogo Institute for Clinical Research\u2008Akashi"},{"name":"Department of Internal Medicine, Kobe University School of Medicine\u2008Kobe"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoichi","family":"Hosomi","sequence":"additional","affiliation":[{"name":"Hyogo Institute for Clinical Research\u2008Akashi"},{"name":"Department of Internal Medicine, Kobe University School of Medicine\u2008Kobe"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumi","family":"Okada","sequence":"additional","affiliation":[{"name":"Hyogo Institute for Clinical Research\u2008Akashi"},{"name":"Department of Internal Medicine, Kobe University School of Medicine\u2008Kobe"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miyako","family":"Kishimoto","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Kobe University School of Medicine\u2008Kobe"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiki","family":"Hozumi","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism, Department of Medicine, Hyogo Medical Center\u2008Akashi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiko","family":"Ishida","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism, Department of Medicine, Hyogo Medical Center\u2008Akashi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsutomu","family":"Kazumi","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Metabolism, Department of Medicine, Hyogo Medical Center\u2008Akashi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuichiro","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Hyogo Institute for Clinical Research\u2008Akashi"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Kasuga","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Kobe University School of Medicine\u2008Kobe"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeaki","family":"Baba","sequence":"additional","affiliation":[{"name":"Hyogo Institute for Clinical Research\u2008Akashi"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/43\/2\/274\/360809\/43-2-274.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/43\/2\/274\/360809\/43-2-274.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:25:17Z","timestamp":1667406317000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/43\/2\/274\/10450\/Enzyme-Linked-Immunosorbent-Assay-Method-for-Human"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,2,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diab.43.2.274","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2,1]]}}}