{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T16:09:37Z","timestamp":1761235777899},"reference-count":0,"publisher":"American Diabetes Association","issue":"5","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,5,1]]},"abstract":"<jats:p>Tumor necrosis factor (TNF) bioactivity was assessed in culture media conditioned with uterine cells collected from control or diabetic rats on days 5 and 8 of pregnancy. On both days, diabetic uterine cells released significantly more biologically active TNF than did control cells, and this activity was significantly decreased by the addition of anti-TNF-\u03b1 antibodies but not by the addition of normal IgG when WEHI 164 cells were used as a target. When uterine tissues from day 5 or day 8 pregnant diabetic rats were tested by Northern blot analysis, TNF-\u03b1 mRNAs were twofold more abundant than in control samples, but the difference was not statistically significant (P = 0.086 and 0.100, respectively). Immunohistochemical analysis of diabetic day 5 uterine sections revealed that most of the TNF-\u03b1 synthesis occurs in the epithelium lining the uterine lumen. Finally, the growth of day-5 embryos in culture medium conditioned with day-5 diabetic uterine cells was significantly reduced when compared with that of embryos in medium conditioned with control cells. Embryonic development was markedly improved when anti-TNF-\u03b1 antibodies were added to the diabetic-cell conditioned medium. Our data support the hypothesis that TNF-\u03b1 may be implicated in the developmental deficiencies observed in preimplantation embryos from pregnant diabetic rats.<\/jats:p>","DOI":"10.2337\/diab.44.5.531","type":"journal-article","created":{"date-parts":[[2013,9,19]],"date-time":"2013-09-19T17:22:06Z","timestamp":1379611326000},"page":"531-536","update-policy":"http:\/\/dx.doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":14,"title":["Possible Role for TNF-\u03b1 in Early Embryopathy Associated With Maternal Diabetes in the Rat"],"prefix":"10.2337","volume":"44","author":[{"given":"Serge","family":"Pampfer","sequence":"first","affiliation":[{"name":"Physiology of Human Reproduction Research Unit, University of Louvain Medical School\u2008Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivo","family":"Vanderheyden","sequence":"additional","affiliation":[{"name":"Physiology of Human Reproduction Research Unit, University of Louvain Medical School\u2008Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan-Der","family":"Wuu","sequence":"additional","affiliation":[{"name":"Physiology of Human Reproduction Research Unit, University of Louvain Medical School\u2008Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurence","family":"Baufays","sequence":"additional","affiliation":[{"name":"Physiology of Human Reproduction Research Unit, University of Louvain Medical School\u2008Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Maillet","sequence":"additional","affiliation":[{"name":"Physiology of Human Reproduction Research Unit, University of Louvain Medical School\u2008Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren\u00e9","family":"De Hertogh","sequence":"additional","affiliation":[{"name":"Physiology of Human Reproduction Research Unit, University of Louvain Medical School\u2008Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/44\/5\/531\/337502\/44-5-531.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/44\/5\/531\/337502\/44-5-531.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:27:50Z","timestamp":1667406470000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/44\/5\/531\/8780\/Possible-Role-for-TNF-in-Early-Embryopathy"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1995,5,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diab.44.5.531","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,5,1]]}}}