{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T19:15:47Z","timestamp":1777749347066,"version":"3.51.4"},"reference-count":0,"publisher":"American Diabetes Association","issue":"5","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,5,1]]},"abstract":"<jats:p>The metabolism of glucose in insulin-secreting cells leads to closure of ATP-sensitive K+ channels (KATP), an event that initiates the insulin secretory process. Defects in insulin secretion are a common feature of non-insulin-dependent diabetes mellitus (NIDDM), and the \u03b2-cell KATP that couples metabolism and membrane potential is a candidate for contributing to the development of this clinically and genetically heterogeneous disorder. We screened a hamster insulinoma cDNA library by low-stringency hybridization with a probe coding for the G-protein-coupled inwardly rectifying K+ channel GIRK1\/KGA and isolated clones encoding a protein, KATP-2, whose sequence is 90% similar to that of the recently described KATP-1, an ATP-sensitive K+ channel expressed in heart and other tissues. RNA blotting showed that KATP mRNA was present in insulin-secreting cells and brain but not in heart. To assess the contribution of KATP-2 to the development of NIDDM, the human KATP-2 gene (symbol KCNJ7) was isolated and mapped to chromosome band 21q22.1 by fluorescence in situ hybridization. A simple tandem repeat DNA polymorphism, D21S1255, was identified in the region of the KATP-2 gene, and linkage studies between this marker and NIDDM were carried out in a group of Mexican-American sib pairs with NIDDM. There was no evidence for linkage between D21S1255 and NIDDM, indicating that KATP-2 is not a major susceptibility gene in this population.<\/jats:p>","DOI":"10.2337\/diab.44.5.592","type":"journal-article","created":{"date-parts":[[2013,9,19]],"date-time":"2013-09-19T17:22:06Z","timestamp":1379611326000},"page":"592-596","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":30,"title":["Isolation of a cDNA Clone Encoding a KATP Channel\u2013Like Protein Expressed in Insulin-Secreting Cells, Localization of the Human Gene to Chromosome Band 21q22.1, and Linkage Studies With NIDDM"],"prefix":"10.2337","volume":"44","author":[{"given":"Meei-Ling","family":"Tsaur","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute and Departments of Physiology and Biochemistry, University of California\u2008San Francisco, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephan","family":"Menzel","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Molecular Biology and Medicine, The University of Chicago\u2008Chicago, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang P","family":"Lai","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience, National Yang-Ming University\u2008Taipei, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"Rafael","family":"Espinosa","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Molecular Biology and Medicine, The University of Chicago\u2008Chicago, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Concannon","sequence":"additional","affiliation":[{"name":"Virginia Mason Research Center\u2008Seattle, Washington"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard S","family":"Spielman","sequence":"additional","affiliation":[{"name":"Department of Genetics, University of Pennsylvania School of Medicine\u2008Philadelphia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Craig L","family":"Hanis","sequence":"additional","affiliation":[{"name":"Human Genetics Center, The University of Texas Health Science Center\u2008Houston, Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy J","family":"Cox","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Molecular Biology and Medicine, The University of Chicago\u2008Chicago, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle M","family":"Le Beau","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Molecular Biology and Medicine, The University of Chicago\u2008Chicago, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael S","family":"German","sequence":"additional","affiliation":[{"name":"Hormone Research Institute and Department of Medicine, University of California\u2008San Francisco, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lily Y","family":"Jan","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Departments of Physiology and Biochemistry, University of California\u2008San Francisco, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graeme I","family":"Bell","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, The University of Chicago\u2008Chicago, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Stoffel","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, The University of Chicago\u2008Chicago, Illinois"},{"name":"Departments of Biochemistry and Molecular Biology and Medicine, The University of Chicago\u2008Chicago, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/44\/5\/592\/337324\/44-5-592.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/44\/5\/592\/337324\/44-5-592.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:27:55Z","timestamp":1667406475000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/44\/5\/592\/8783\/Isolation-of-a-cDNA-Clone-Encoding-a-KATP-Channel"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1995,5,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diab.44.5.592","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,5,1]]}}}