{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:04:11Z","timestamp":1791187451702,"version":"4.1.0"},"reference-count":0,"publisher":"American Diabetes Association","issue":"3","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,3,1]]},"abstract":"<jats:p>Islet duodenal homeobox 1 (IDX-1\/PF-1\/STF-1\/PDX-1), a homeodomain protein that transactivates the insulin promoter, has been shown by targeted gene ablation to be required for pancreatic development. After 90% pancreatectomy (Px), the adult pancreas regenerates in a process recapitulating embryonic development, starting with a burst of proliferation in the epithelium of the common pancreatic duct. In this model, IDX-1 mRNA was detected by semiquantitative reverse transcription-polymerase chain reaction in total RNA from isolated common pancreatic ducts at levels 10% of those of isolated islets. The IDX-1 mRNA levels were not significantly different for common pancreatic ducts of Px, sham Px, and unoperated rats and did not change with time after surgery. By immunoblot analysis, IDX-1 protein was only faintly detected in these ducts 1 and 7 days after Px or sham Px but was easily detected at 2 and 3 days after Px. Similarly, IDX-1 immunostaining was barely detectable in sham or unoperated ducts but was strong in ducts at 2-3 days after Px. The increase of IDX-1 immunostaining followed that of BrdU incorporation (proliferation). These results indicate a posttranscriptional regulation of the IDX-1 expression in ducts. In addition, islets isolated 3-7 d after Px showed higher IDX-1 protein expression than control islets. Thus, in pancreatic regeneration IDX-1 is upregulated in newly divided ductal cells as well as in islets. The timing of enhanced expression of IDX-1 implies that IDX-1 is not important in the initiation of regeneration but may be involved in the differentiation of ductal cells to beta-cells.<\/jats:p>","DOI":"10.2337\/diabetes.48.3.507","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"507-513","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":261,"title":["The homeodomain protein IDX-1 increases after an early burst of proliferation during pancreatic regeneration."],"prefix":"10.2337","volume":"48","author":[{"given":"A","family":"Sharma","sequence":"first","affiliation":[{"name":"E.P. Joslin Research Laboratories, Joslin Diabetes Center, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D H","family":"Zangen","sequence":"additional","affiliation":[{"name":"E.P. Joslin Research Laboratories, Joslin Diabetes Center, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Reitz","sequence":"additional","affiliation":[{"name":"E.P. Joslin Research Laboratories, Joslin Diabetes Center, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Taneja","sequence":"additional","affiliation":[{"name":"E.P. Joslin Research Laboratories, Joslin Diabetes Center, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M E","family":"Lissauer","sequence":"additional","affiliation":[{"name":"E.P. Joslin Research Laboratories, Joslin Diabetes Center, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C P","family":"Miller","sequence":"additional","affiliation":[{"name":"E.P. Joslin Research Laboratories, Joslin Diabetes Center, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G C","family":"Weir","sequence":"additional","affiliation":[{"name":"E.P. Joslin Research Laboratories, Joslin Diabetes Center, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J F","family":"Habener","sequence":"additional","affiliation":[{"name":"E.P. Joslin Research Laboratories, Joslin Diabetes Center, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Bonner-Weir","sequence":"additional","affiliation":[{"name":"E.P. Joslin Research Laboratories, Joslin Diabetes Center, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/48\/3\/507\/364181\/10078550.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/48\/3\/507\/364181\/10078550.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:47:14Z","timestamp":1667407634000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/48\/3\/507\/12212\/The-homeodomain-protein-IDX-1-increases-after-an"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1999,3,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diabetes.48.3.507","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,3,1]]}}}