{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T11:37:36Z","timestamp":1792409856257,"version":"4.4.0"},"reference-count":0,"publisher":"American Diabetes Association","issue":"5","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1999,5,1]]},"abstract":"<jats:p>The discovery of uncoupling protein (UCP)-2, a ubiquitously expressed protein homologous to UCP-1, has raised the possibility that energy balance of cells might be regulated in tissues other than brown adipocytes. In normal pancreatic islets, UCP-2 is upregulated by leptin and is low in leptin-resistant islets of ZDF rats. To determine whether UCP-2 does, in fact, have uncoupling activity and, if so, whether such activity would favorably influence the abnormalities in leptin-unresponsive UCP-2-underexpressing islets of diabetic ZDF rats, we transferred the UCP-2 gene to the islets of diabetic ZDF rats and lean (+\/+) ZDF control rats. Although ATP was reduced by 23% in both groups of islets, the ATP:ADP ratio increased by 42 and 141%, respectively. [3H]palmitate oxidation was increased by 50%, and [3H]glucose oxidation was 42-63% higher. Preproinsulin mRNA was 2.9-fold above control levels, and glucose-stimulated insulin secretion, which was negligible in control ZDF rat islets, was improved in UCP-2-overexpressing islets. The high fat content of the islets was not reduced, however. We conclude that UCP-2 has uncoupling function when overexpressed in leptin-insensitive islets and that its overexpression corrects the underexpression of the insulin gene and ameliorates glucose-stimulated insulin secretion, possibly by increasing the ATP:ADP ratio.<\/jats:p>","DOI":"10.2337\/diabetes.48.5.1020","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"1020-1025","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":56,"title":["Adenovirus-mediated overexpression of uncoupling protein-2 in pancreatic islets of Zucker diabetic rats increases oxidative activity and improves beta-cell function."],"prefix":"10.2337","volume":"48","author":[{"given":"M Y","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, and the Veterans Administration Medical Center, Dallas 75235-8854, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Shimabukuro","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, and the Veterans Administration Medical Center, Dallas 75235-8854, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, and the Veterans Administration Medical Center, Dallas 75235-8854, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K Y","family":"Trinh","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, and the Veterans Administration Medical Center, Dallas 75235-8854, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J L","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, and the Veterans Administration Medical Center, Dallas 75235-8854, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C B","family":"Newgard","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, and the Veterans Administration Medical Center, Dallas 75235-8854, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R H","family":"Unger","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, and the Veterans Administration Medical Center, Dallas 75235-8854, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/48\/5\/1020\/364291\/10331406.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/48\/5\/1020\/364291\/10331406.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:47:51Z","timestamp":1667407671000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/48\/5\/1020\/12262\/Adenovirus-mediated-overexpression-of-uncoupling"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1999,5,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diabetes.48.5.1020","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,5,1]]}}}