{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T09:45:01Z","timestamp":1787737501780,"version":"build-2784847793"},"reference-count":0,"publisher":"American Diabetes Association","issue":"3","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,3,1]]},"abstract":"<jats:p>Previous work has shown that interleukin-1beta (IL-1beta) alters protein expression in beta-cells. This alteration is associated with cell death in isolated rat islets but not in isolated rat beta-cells. We examined whether IL-1beta pretreatment of isolated beta-cells influences their sensitivity to toxic agents. After a 24-h culture with IL-1beta (30 U\/ml), beta-cells exhibited a lower expression of the beta-cell-specific protein transcription factor pancreatic and duodenal homeobox gene (PDX)-1, glucose transporter GLUT2, and proinsulin convertase PC2, with a marked reduction (60-70%) in glucose-induced insulin production and selective sensitivity to the toxins alloxan (ALX) and streptozotocin (STZ). On the other hand, the cells presented an increased expression of Mn-superoxide dismutase, heat shock protein 70, inducible heme oxygenase, and inducible nitrite oxide synthase. This IL-1beta-induced alteration in beta-cell phenotype resulted in a reduced cellular sensitivity to the beta-cell-specific toxins ALX and STZ; the production of nontoxic conditions of nitric oxide (NO) also rendered the cells less susceptible to radical-induced damage. Exposure to IL-1beta can thus protect beta-cells against conditions that cause necrosis; however, it did not protect against apoptosis induced by the additional presence of interferon-gamma or tumor necrosis factor-alpha. Release of IL-1beta in the endocrine pancreas is thus not necessarily the cause of massive NO-dependent beta-cell destruction. On the contrary, IL-1beta may protect these cells against necrosis, though with a loss of their characteristic phenotype and homeostatic functions.<\/jats:p>","DOI":"10.2337\/diabetes.49.3.340","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"340-345","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":55,"title":["Interleukin-1beta-induced alteration in a beta-cell phenotype can reduce cellular sensitivity to conditions that cause necrosis but not to cytokine-induced apoptosis."],"prefix":"10.2337","volume":"49","author":[{"given":"Z","family":"Ling","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Diabetes Research Center, Vrije Universiteit Brussel, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Van de Casteele","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Diabetes Research Center, Vrije Universiteit Brussel, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D L","family":"Eizirik","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Diabetes Research Center, Vrije Universiteit Brussel, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D G","family":"Pipeleers","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Diabetes Research Center, Vrije Universiteit Brussel, Belgium."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/49\/3\/340\/365130\/10868954.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/49\/3\/340\/365130\/10868954.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:50:17Z","timestamp":1667407817000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/49\/3\/340\/10678\/Interleukin-1beta-induced-alteration-in-a-beta"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,3,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diabetes.49.3.340","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,3,1]]}}}