{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T10:14:04Z","timestamp":1790936044166,"version":"4.1.0"},"reference-count":0,"publisher":"American Diabetes Association","issue":"4","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,4,1]]},"abstract":"<jats:p>The homeodomain transcription factor encoded by the pancreatic and duodenal homeobox gene-1 (Ipf-1) is essential for pancreatic ontogenesis. Whether Ipf-1 is also involved in the neogenesis of beta-cells in the adult pancreas is unknown. We examined whether Ipf-1 is expressed in adult human pancreatic ducts, which are thought to generate new beta-cells. In tissue sections, virtually all duct cells were immunopositive for Ipf-1, as were the islet beta-cells but not the acinar cells. After isolation and culture, both duct and islet cell preparations contained the Ipf-1 immunoreactive proteins p42 and p45 (42 and 45 kDa, respectively) in similar proportions, but the expression levels were twofold lower in duct cells. After 4 h of labeling, the endocrine cells exhibited a sevenfold higher phosphorylation of p42 than the duct cells, whereas p45 was phosphorylated only in endocrine cells. Homeobox binding transcription factor complexes with Ipf-1 in duct cells differed from those in endocrine cells in terms of gel mobility, sequence specificity, and affinity. The observed similarities in Ipf-1 expression by adult human pancreatic duct cells and endocrine cells may reflect their common ontogenic origin, whereas the differences in Ipf-1 phosphorylation and complex formation may correlate with their divergent differentiation.<\/jats:p>","DOI":"10.2337\/diabetes.49.4.571","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"571-579","update-policy":"https:\/\/doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":54,"title":["Adult human pancreatic duct and islet cells exhibit similarities in expression and differences in phosphorylation and complex formation of the homeodomain protein Ipf-1."],"prefix":"10.2337","volume":"49","author":[{"given":"H","family":"Heimberg","sequence":"first","affiliation":[{"name":"Diabetes Research Center, Vrije Universiteit Brussel, Belgium. hheimber@vub.vub.ac.be"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Bouwens","sequence":"additional","affiliation":[{"name":"Diabetes Research Center, Vrije Universiteit Brussel, Belgium. hheimber@vub.vub.ac.be"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Heremans","sequence":"additional","affiliation":[{"name":"Diabetes Research Center, Vrije Universiteit Brussel, Belgium. hheimber@vub.vub.ac.be"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Van De Casteele","sequence":"additional","affiliation":[{"name":"Diabetes Research Center, Vrije Universiteit Brussel, Belgium. hheimber@vub.vub.ac.be"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V","family":"Lefebvre","sequence":"additional","affiliation":[{"name":"Diabetes Research Center, Vrije Universiteit Brussel, Belgium. hheimber@vub.vub.ac.be"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Pipeleers","sequence":"additional","affiliation":[{"name":"Diabetes Research Center, Vrije Universiteit Brussel, Belgium. hheimber@vub.vub.ac.be"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/49\/4\/571\/365235\/10871194.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/49\/4\/571\/365235\/10871194.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:50:34Z","timestamp":1667407834000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/49\/4\/571\/10395\/Adult-human-pancreatic-duct-and-islet-cells"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2000,4,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diabetes.49.4.571","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,4,1]]}}}