{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T14:33:15Z","timestamp":1720794795976},"reference-count":0,"publisher":"American Diabetes Association","issue":"9","content-domain":{"domain":["diabetesjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,9,1]]},"abstract":"<jats:p>The D variant of encephalomyocarditis (EMC-D) virus causes diabetes in susceptible mice by direct cytolysis of pancreatic beta-cells. cDNA covering the major outer capsid protein (VP1) of the EMC-D virus was cloned into Mycobacterium bovis bacillus Calmette-Guerin (BCG). None of the SJL\/J mice immunized with live recombinant BCG-VP1 (rBCG-VP1) became diabetic when challenged with the highly diabetogenic EMC-D virus, but the control mice inoculated with normal BCG developed diabetes during the same challenge. VP1-specific antibodies (including neutralizing antibodies) were markedly increased over time and reached the maximum titer at week 10 after a single immunization. The plateau of the titer lasted longer than 4 weeks. Mice and guinea pigs immunized with live rBCG-VP1 showed strong delayed-type hypersensitivity to the VP1 of the EMC-D virus. The preventive immunity still worked effectively 10 months after the primary immunization. At that time, the VP1-specific antibody was almost undetectable in the bloodstream, but a large number of VP1-specific lymphocytes was found in the spleen of the immunized mice. Our results show that live rBCG-VP1 elicits effective humoral and long-lasting cellular immune responses against EMC-D virus infection that results in the prevention of virus-induced diabetes in susceptible mice.<\/jats:p>","DOI":"10.2337\/diabetes.49.9.1459","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T19:04:22Z","timestamp":1173207862000},"page":"1459-1467","update-policy":"http:\/\/dx.doi.org\/10.2337\/ada-journal-policies","source":"Crossref","is-referenced-by-count":10,"title":["Prevention of encephalomyocarditis virus-induced diabetes by live recombinant Mycobacterium bovis bacillus Calmette-Gu\u00e9rin in susceptible mice."],"prefix":"10.2337","volume":"49","author":[{"given":"B K","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Microbiology, Hannam University, Taejon City, South Korea."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S H","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hannam University, Taejon City, South Korea."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G H","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hannam University, Taejon City, South Korea."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S J","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hannam University, Taejon City, South Korea."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B H","family":"Hyun","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hannam University, Taejon City, South Korea."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y K","family":"Choe","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hannam University, Taejon City, South Korea."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y S","family":"Bae","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Hannam University, Taejon City, South Korea."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1167","container-title":["Diabetes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.org\/diabetes\/diabetes\/article-pdf\/49\/9\/1459\/365092\/10969829.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article-pdf\/49\/9\/1459\/365092\/10969829.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T16:07:59Z","timestamp":1667405279000},"score":1,"resource":{"primary":{"URL":"https:\/\/diabetesjournals.org\/diabetes\/article\/49\/9\/1459\/10784\/Prevention-of-encephalomyocarditis-virus-induced"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,9,1]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2000,9,1]]}},"URL":"https:\/\/doi.org\/10.2337\/diabetes.49.9.1459","relation":{},"ISSN":["0012-1797","1939-327X"],"issn-type":[{"value":"0012-1797","type":"print"},{"value":"1939-327X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,9,1]]}}}